Utilization of an Automated Pipetting System in the Cell
Line-Based Screening of the Activity of a DNA-Damaging
Anti-Tumour Drug

J 2014

Utilization of an Automated Pipetting System in the Cell Line-Based Screening of the Activity of a DNA-Damaging Anti-Tumour Drug

SUCHÁNKOVÁ, T.; Petra OVESNÁ; Pounami SAMADDER a K. SOUČEK

Základní údaje

Originální název

Utilization of an Automated Pipetting System in the Cell Line-Based Screening of the Activity of a DNA-Damaging Anti-Tumour Drug

Autoři

SUCHÁNKOVÁ, T.; Petra OVESNÁ ; Pounami SAMADDER a K. SOUČEK

Vydání

Folia biologica, Prague, Charles University, 2014, 0015-5500

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.000

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/14:00078212

Organizační jednotka

Lékařská fakulta

UT WoS

000343275800008

Klíčová slova anglicky

cell line screen; drug treatment; liquid handling station; epMotion (R)

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 1. 2015 14:43, Soňa Böhmová

Anotace

V originále

The principles of large screening strategies, which are developed by industrial companies, have been recently adopted by researchers in the fields of molecular biology and oncology as invaluable tools for translational medicine. The declining costs of laboratory robotic machines have allowed high-throughput screening to become more available to academic centres with limited resources. Here, we describe how a robotic conventional liquid handling system could be used on a daily basis in laboratories to obtain consistent and reproducible results. Our approach allowed us to quickly screen a panel of more than 20 tumorigenic and non-tumorigenic cell lines for their responses to hydroxyurea, which is a DNA-damaging anticancer therapeutic drug. The format of 384-well microplates was used for manual cell seeding, and the effect of hydroxyurea was screened at multiple concentrations. The fluorescence- based CyQuant assay was employed as the readout method to analyse the cellular DNA content. The effectiveness of our approach was demonstrated in the experimental results.

Návaznosti

EE2.3.20.0185, projekt VaV

Název: Centrum analýz a modelování tkání a orgánů

Citovat

SUCHÁNKOVÁ, T.; Petra OVESNÁ; Pounami SAMADDER a K. SOUČEK. Utilization of an Automated Pipetting System in the Cell Line-Based Screening of the Activity of a DNA-Damaging Anti-Tumour Drug. Folia biologica. Prague: Charles University, 2014, roč. 60, Suppl. 1, s. 50-55. ISSN 0015-5500.

@article{1216134,
   author = {Suchánková, T. and Ovesná, Petra and Samadder, Pounami and Souček, K.},
   article_location = {Prague},
   article_number = {Suppl. 1},
   keywords = {cell line screen; drug treatment; liquid handling station; epMotion (R)},
   language = {eng},
   issn = {0015-5500},
   journal = {Folia biologica},
   title = {Utilization of an Automated Pipetting System in the Cell Line-Based Screening of the Activity of a DNA-Damaging Anti-Tumour Drug},
   volume = {60},
   year = {2014}
}

TY  - JOUR
ID  - 1216134
AU  - Suchánková, T. - Ovesná, Petra - Samadder, Pounami - Souček, K.
PY  - 2014
TI  - Utilization of an Automated Pipetting System in the Cell Line-Based Screening of the Activity of a DNA-Damaging Anti-Tumour Drug
JF  - Folia biologica
VL  - 60
IS  - Suppl. 1
SP  - 50-55
EP  - 50-55
PB  - Charles University
SN  - 00155500
KW  - cell line screen
KW  - drug treatment
KW  - liquid handling station
KW  - epMotion (R)
N2  - The principles of large screening strategies, which are developed by industrial companies, have been recently adopted by researchers in the fields of molecular biology and oncology as invaluable tools for translational medicine. The declining costs of laboratory robotic machines have allowed high-throughput screening to become more available to academic centres with limited resources. Here, we describe how a robotic conventional liquid handling system could be used on a daily basis in laboratories to obtain consistent and reproducible results. Our approach allowed us to quickly screen a panel of more than 20 tumorigenic and non-tumorigenic cell lines for their responses to hydroxyurea, which is a DNA-damaging anticancer therapeutic drug. The format of 384-well microplates was used for manual cell seeding, and the effect of hydroxyurea was screened at multiple concentrations. The fluorescence- based CyQuant assay was employed as the readout method to analyse the cellular DNA content. The effectiveness of our approach was demonstrated in the experimental results.
ER  -

SUCHÁNKOVÁ, T.; Petra OVESNÁ; Pounami SAMADDER a K. SOUČEK. Utilization of an Automated Pipetting System in the Cell Line-Based Screening of the Activity of a DNA-Damaging Anti-Tumour Drug. \textit{Folia biologica}. Prague: Charles University, 2014, roč.~60, Suppl. 1, s.~50-55. ISSN~0015-5500.

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