Další formáty:
BibTeX
LaTeX
RIS
@article{1216380,
author = {Kuglík, Petr and Smetana, Jan and Vallová, Vladimíra and Mouková, Lucie and Kašíková, Kateřina and Cvanová, Michaela and Brožová, Lucie},
article_location = {USA},
article_number = {8},
keywords = {Cervical carcinoma; whole-genome profiling; CGH+SNP microarrays; HPV-FISH; copy number alterations},
language = {eng},
issn = {1936-2625},
journal = {International Journal of Clinical and Experimental Pathology},
title = {Genome-wide screening of DNA copy number alterations in cervical carcinoma patients with CGH+SNP microarrays and HPV-FISH},
url = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4152070},
volume = {7},
year = {2014}
}
TY - JOUR
ID - 1216380
AU - Kuglík, Petr - Smetana, Jan - Vallová, Vladimíra - Mouková, Lucie - Kašíková, Kateřina - Cvanová, Michaela - Brožová, Lucie
PY - 2014
TI - Genome-wide screening of DNA copy number alterations in cervical carcinoma patients with CGH+SNP microarrays and HPV-FISH
JF - International Journal of Clinical and Experimental Pathology
VL - 7
IS - 8
SP - 5071-5082
EP - 5071-5082
PB - e-Century Publishing Corporation, Madiso
SN - 19362625
KW - Cervical carcinoma
KW - whole-genome profiling
KW - CGH+SNP microarrays
KW - HPV-FISH
KW - copy number alterations
UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4152070
L2 - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4152070
N2 - Alterations in the genome that lead to changes in DNA sequence copy number are characteristic features of solid tumors. We used CGH+SNP microarray and HPV-FISH techniques for detailed screening of copy number alterations (CNAs) in a cohort of 26 patients with cervical carcinoma (CC). This approach identified CNAs in 96.2% (25/26) of tumors. Array-CGH discovered CNAs in 73.1% (19/26) of samples, HPV-FISH experiments revealed CNAs in additional 23.1% (6/26) of samples. Common gains of genetic sequences were observed in 3q (50.0%), 1q (42.4%), 19q (23.1%), while losses were frequently found in 11q (30.8%), 4q (23.1%) and 13q (19.2%). Chromosomal regions involved in loss of heterozygosity were observed in 15.4% of samples in 8q21, 11q23, 14q21 and 18q12.2. Incidence of gain 3q was associated with HPV 16 and HPV 18 positive samples and simultaneous presence of gain 1q (P = 0.033). We did not found a correlation between incidence of CNAs identified by array-CGH and HPV strain infection and incidence of lymph node metastases. Subsequently, HPV-FISH was used for validation of array-CGH results in 23 patients for incidence of hTERC (3q26) and MYC (8q24) amplification. Using HPV-FISH, we found chromosomal lesions of hTERC in 87.0% and MYC in 65.2% of specimens. Our findings confirmed the important role of HPV infection and specific genomic alterations in the development of invasive cervical cancer. This study also indicates that CGH+SNP microarrays allow detecting genome-wide CNAs and copy-neutral loss of heterozygosity more precisely, however, it may be less sensitive than FISH in samples with low level clonal CNAs.
ER -
KUGLÍK, Petr, Jan SMETANA, Vladimíra VALLOVÁ, Lucie MOUKOVÁ, Kateřina KAŠÍKOVÁ, Michaela CVANOVÁ a Lucie BROŽOVÁ. Genome-wide screening of DNA copy number alterations in cervical carcinoma patients with CGH+SNP microarrays and HPV-FISH. \textit{International Journal of Clinical and Experimental Pathology}. USA: e-Century Publishing Corporation, Madiso, 2014, roč.~7, č.~8, s.~5071-5082. ISSN~1936-2625.
|
