Separation of Methicillin-Resistant from
Methicillin-Susceptible Staphylococcus aureus by
Electrophoretic Methods in Fused Silica Capillaries Etched with
Supercritical Water

J 2014

Separation of Methicillin-Resistant from Methicillin-Susceptible Staphylococcus aureus by Electrophoretic Methods in Fused Silica Capillaries Etched with Supercritical Water

HORKÁ, Marie; Pavel KARÁSEK; Filip RŮŽIČKA; Milada DVOŘÁČKOVÁ; Martina SITTOVÁ et al.

Základní údaje

Originální název

Separation of Methicillin-Resistant from Methicillin-Susceptible Staphylococcus aureus by Electrophoretic Methods in Fused Silica Capillaries Etched with Supercritical Water

Autoři

HORKÁ, Marie; Pavel KARÁSEK; Filip RŮŽIČKA ; Milada DVOŘÁČKOVÁ; Martina SITTOVÁ a Michal ROTH

Vydání

Analytical Chemistry, Washington, American Chemical Society, 2014, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.636

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/14:00073247

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

FLIGHT MASS-SPECTROMETRY; BACTERIAL ADHESION; ZONE-ELECTROPHORESIS; ISOELECTRIC POINT; CHROMOGENIC AGAR; SPECTRA MRSA; UV DETECTION; MICROORGANISMS; STRAINS; IDENTIFICATION

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 5. 2015 16:06, Soňa Böhmová

Anotace

V originále

Identification and prevention of Staphylococcus aureus-caused infections may benefit from a fast and dependable method to distinguish between the methicillin-resistant (MRSA) and methicillin susceptible (MSSA) S. aureus strains. The current methods involving polymerase chain reaction and/or other molecular tests are usually laborious and time-consuming. We describe here a fasgt and low-cost method employing capillary zone electrophoresis (CZE) to distinguish between MRSA and MSSA. The method makes use of a supecritical water-treated fused silica capillary, the inner surface of which has subsequently been modified with (3 glycidyloxypropyl)trimethoxysilane. With optimized proportions fo suitable additives to the background electrolyte, a CZE separation of MRSA from MSSA may be completed within 12 min. The cells were baseline-resolved, and resolution was determined to be 3.61. the isoelectric points of MSSA and MRSA were found to be the same for both groups of these strains, pI= 3.4.

Návaznosti

NT13242, projekt VaV

Název: Polymikrobiální biofilm jako příčina chronických močových infekcí a selhávání terapie: kultivační a PCR-DGGE diagnostika, význam a možnosti řešení

Investor: Ministerstvo zdravotnictví ČR, Polymikrobiální biofilm jako příčina chronických močových infekcí a selhávání terapie: kultivační a PCR-DGGE diagnostika, význam a možnosti řešení

VG20112015021, projekt VaV

Název: Vývoj instrumentálních metodických postupů rychlé detekce a identifikace biologických agens v reálných vzorcích (Akronym: Detekce a identifikace biol. agens)

Investor: Ministerstvo vnitra ČR, Vývoj instrumentálních metodických postupů rychlé detekce a identifikace biologických agens v reálných vzorcích

Citovat

HORKÁ, Marie; Pavel KARÁSEK; Filip RŮŽIČKA; Milada DVOŘÁČKOVÁ; Martina SITTOVÁ a Michal ROTH. Separation of Methicillin-Resistant from Methicillin-Susceptible Staphylococcus aureus by Electrophoretic Methods in Fused Silica Capillaries Etched with Supercritical Water. Analytical Chemistry. Washington: American Chemical Society, 2014, roč. 86, č. 19, s. 9701-9708. ISSN 0003-2700. Dostupné z: https://doi.org/10.1021/ac502254f.

@article{1217609,
   author = {Horká, Marie and Karásek, Pavel and Růžička, Filip and Dvořáčková, Milada and Sittová, Martina and Roth, Michal},
   article_location = {Washington},
   article_number = {19},
   doi = {https://doi.org/10.1021/ac502254f},
   keywords = {FLIGHT MASS-SPECTROMETRY; BACTERIAL ADHESION; ZONE-ELECTROPHORESIS; ISOELECTRIC POINT; CHROMOGENIC AGAR; SPECTRA MRSA; UV DETECTION; MICROORGANISMS; STRAINS; IDENTIFICATION},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Separation of Methicillin-Resistant from Methicillin-Susceptible Staphylococcus aureus by Electrophoretic Methods in Fused Silica Capillaries Etched with Supercritical Water},
   url = {http://pubs.acs.org/doi/abs/10.1021/ac502254f},
   volume = {86},
   year = {2014}
}

TY  - JOUR
ID  - 1217609
AU  - Horká, Marie - Karásek, Pavel - Růžička, Filip - Dvořáčková, Milada - Sittová, Martina - Roth, Michal
PY  - 2014
TI  - Separation of Methicillin-Resistant from Methicillin-Susceptible Staphylococcus aureus by Electrophoretic Methods in Fused Silica Capillaries Etched with Supercritical Water
JF  - Analytical Chemistry
VL  - 86
IS  - 19
SP  - 9701-9708
EP  - 9701-9708
PB  - American Chemical Society
SN  - 00032700
KW  - FLIGHT MASS-SPECTROMETRY
KW  - BACTERIAL ADHESION
KW  - ZONE-ELECTROPHORESIS
KW  - ISOELECTRIC POINT
KW  - CHROMOGENIC AGAR
KW  - SPECTRA MRSA
KW  - UV DETECTION
KW  - MICROORGANISMS
KW  - STRAINS
KW  - IDENTIFICATION
UR  - http://pubs.acs.org/doi/abs/10.1021/ac502254f
L2  - http://pubs.acs.org/doi/abs/10.1021/ac502254f
N2  - Identification and prevention of Staphylococcus aureus-caused infections may benefit from a fast and dependable method to distinguish between the methicillin-resistant (MRSA) and methicillin susceptible (MSSA) S. aureus strains. The current methods involving polymerase chain reaction and/or other molecular tests are usually laborious and time-consuming. We describe here a fasgt and low-cost method employing capillary zone electrophoresis (CZE) to distinguish between MRSA and MSSA. The method makes use of a supecritical water-treated fused silica capillary, the inner surface of which has subsequently been modified with (3 glycidyloxypropyl)trimethoxysilane. With optimized proportions fo suitable additives to the background electrolyte, a CZE separation of MRSA from MSSA may be completed within 12 min. The cells were baseline-resolved, and resolution was determined to be 3.61. the isoelectric points of MSSA and MRSA were found to be the same for both groups of these strains, pI= 3.4.
ER  -

HORKÁ, Marie; Pavel KARÁSEK; Filip RŮŽIČKA; Milada DVOŘÁČKOVÁ; Martina SITTOVÁ a Michal ROTH. Separation of Methicillin-Resistant from Methicillin-Susceptible Staphylococcus aureus by Electrophoretic Methods in Fused Silica Capillaries Etched with Supercritical Water. \textit{Analytical Chemistry}. Washington: American Chemical Society, 2014, roč.~86, č.~19, s.~9701-9708. ISSN~0003-2700. Dostupné z: https://doi.org/10.1021/ac502254f.

Přehled o publikaci