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@article{1217790,
author = {Gumulec, Jaromír and Fojtů, Michaela and Raudenská, Martina and Sztalmachová, Markéta and Skotáková, Anna and Vlachova, Jana and Skalickova, Sylvie and Nejdl, Lukáš and Kopel, Pavel and Knopfová, Lucia and Adam, Vojtech and Kizek, Rene and Stiborova, Marie and Babula, Petr and Masařík, Michal},
article_location = {Basel},
article_number = {12},
doi = {http://dx.doi.org/10.3390/ijms151222960},
keywords = {Apoferritin; Cancer; Cardiotoxicity; Doxorubicin; Encapsulation; Liposome; Modification},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {Modulation of induced cytotoxicity of doxorubicin by using apoferritin and liposomal cages},
volume = {15},
year = {2014}
}
TY - JOUR
ID - 1217790
AU - Gumulec, Jaromír - Fojtů, Michaela - Raudenská, Martina - Sztalmachová, Markéta - Skotáková, Anna - Vlachova, Jana - Skalickova, Sylvie - Nejdl, Lukáš - Kopel, Pavel - Knopfová, Lucia - Adam, Vojtech - Kizek, Rene - Stiborova, Marie - Babula, Petr - Masařík, Michal
PY - 2014
TI - Modulation of induced cytotoxicity of doxorubicin by using apoferritin and liposomal cages
JF - International Journal of Molecular Sciences
VL - 15
IS - 12
SP - 22960-22977
EP - 22960-22977
PB - Multidisciplinary Digital Publishing Institute
SN - 14220067
KW - Apoferritin
KW - Cancer
KW - Cardiotoxicity
KW - Doxorubicin
KW - Encapsulation
KW - Liposome
KW - Modification
N2 - Doxorubicin is an effective chemotherapeutic drug, however, its toxicity is a significant limitation in therapy. Encapsulation of doxorubicin inside liposomes or ferritin cages decreases cardiotoxicity while maintaining anticancer potency. We synthesized novel apoferritin- and liposome-encapsulated forms of doxorubicin (“Apodox” and “lip-8-dox”) and compared its toxicity with doxorubicin and Myocet on prostate cell lines. Three different prostatic cell lines PNT1A, 22Rv1, and LNCaP were chosen. The toxicity of the modified doxorubicin forms was compared to conventional doxorubicin using the MTT assay, real-time cell impedance-based cell growth method (RTCA), and flow cytometry. The efficiency of doxorubicin entrapment was 56% in apoferritin cages and 42% in the liposome carrier. The accuracy of the RTCA system was verified by flow-cytometric analysis of cell viability. The doxorubicin half maximal inhibition concentrations (IC50) were determined as 170.5, 234.0, and 169.0 nM for PNT1A, 22Rv1, and LNCaP, respectively by RTCA. Lip8-dox is less toxic on the non-tumor cell line PNT1A compared to doxorubicin, while still maintaining the toxicity to tumorous cell lines similar to doxorubicin or epirubicin (IC50 = 2076.7 nM for PNT1A vs. 935.3 and 729.0 nM for 22Rv1 and LNCaP). Apodox IC50 was determined as follows: 603.1, 1344.2, and 931.2 nM for PNT1A, 22Rv1, and LNCaP.
ER -
GUMULEC, Jaromír, Michaela FOJTŮ, Martina RAUDENSKÁ, Markéta SZTALMACHOVÁ, Anna SKOTÁKOVÁ, Jana VLACHOVA, Sylvie SKALICKOVA, Lukáš NEJDL, Pavel KOPEL, Lucia KNOPFOVÁ, Vojtech ADAM, Rene KIZEK, Marie STIBOROVA, Petr BABULA a Michal MASAŘÍK. Modulation of induced cytotoxicity of doxorubicin by using apoferritin and liposomal cages. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, roč.~15, č.~12, s.~22960-22977. ISSN~1422-0067. doi:10.3390/ijms151222960. 2014.
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