KOHOUTOVA, Darina, David ŠMAJS, Paula MORAVKOVA, Jiri CYRANY, Monika MORAVKOVA, Miroslava FORSTLOVA, Michal CIHAK, Stanislav REJCHRT a Jan BURES. Escherichia coli strains of phylogenetic group B2 and D and bacteriocin production are associated with advanced colorectal neoplasia. BMC Infectious Diseases, London: BioMed Central, 2014, roč. 14, č. 733, s. 1-8. ISSN 1471-2334. doi:10.1186/s12879-014-0733-7.
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Základní údaje
Originální název Escherichia coli strains of phylogenetic group B2 and D and bacteriocin production are associated with advanced colorectal neoplasia
Autoři KOHOUTOVA, Darina (203 Česká republika), David ŠMAJS (203 Česká republika, garant, domácí), Paula MORAVKOVA (203 Česká republika), Jiri CYRANY (203 Česká republika), Monika MORAVKOVA (203 Česká republika), Miroslava FORSTLOVA (203 Česká republika), Michal CIHAK (203 Česká republika), Stanislav REJCHRT (203 Česká republika) a Jan BURES (203 Česká republika).
Vydání BMC Infectious Diseases, London, BioMed Central, 2014, 1471-2334.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10600 1.6 Biological sciences
Stát vydavatele Spojené království
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 2.613
Kód RIV RIV/00216224:14110/14:00078631
Organizační jednotka Lékařská fakulta
Doi http://dx.doi.org/10.1186/s12879-014-0733-7
Klíčová slova anglicky Escherichia coli; Colicins; Bacteriocins; Colorectal adenoma; Colorectal carcinoma
Štítky EL OK
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Soňa Böhmová, učo 232884. Změněno: 23. 1. 2015 14:19.
Anotace
Background: Colorectal cancer (CRC) is the 3rd most common cancer worldwide and the Czech Republic has the 6th highest incidence of CRC worldwide. Large intestinal microbiota play in its etiopathogenesis important role. Bacteriocins are proteins, produced by bacteria from the Enterobacteriaceae family. The aim of our prospective study was to assess the colonization of large intestinal mucosa by Escherichia coli strains and to investigate their bacteriocin production. Methods: A total of 30 consecutive patients with colorectal adenoma, CRA (17 men, 13 women, aged 39–79, mean age 63 +/- 9), 30 patients with CRC (23 men, 7 women, aged 38–86, mean age 67 +/- 11) and 20 healthy controls (9 men, 11 women, age 23–84, mean age 55 +/- 15) were enrolled into prospective study. Mucosal biopsies were taken in the caecum, transverse colon and rectum during pancolonoscopy. Microbiological culture, isolation and identification of bacteria followed. Bacteriocin production was assessed by growth inhibition of indicator strains E. coli K12-Row, E. coli C6 (phi), and Shigella sonnei 17. Identification of bacteriocin-encoding determinants and E. coli phylogroups was performed using PCR methods. Results: A total of 622 strains were isolated and further investigated. A significantly higher frequency of simultaneous production of colicins and microcins was revealed in the group of patients with CRC, when compared to patients with CRA, p = 0.031. A significantly higher frequency of E. coli phylogroup D was found in patients with CRC, when compared to controls, p = 0.044. A significantly higher prevalence of bacteriocinogeny was confirmed in patients with advanced adenoma when compared to patients with non-advanced adenoma, p = 0.010. Increasing bacteriocinogeny was associated with an increasing stage of CRC (assessed according to TNM classification). Either E. coli phylogroup B2 or E. coli phylogroup D were isolated in biopsies of patients with right-sided CRC. A statistically higher incidence of E. coli phylogroup B2 was found in patients with right-sided CRC when compared to patients with left-sided CRC, p=0.028. Conclusions: Large intestinal mucosa of patients with more advanced colorectal neoplasia is colonized with more virulent strains of E. coli and higher production of bacteriocins is observed in these patients when compared to those with less advanced colorectal neoplasia.
VytisknoutZobrazeno: 13. 12. 2019 06:26