ZÁLEŠÁK, Jan, Morgane LOURDIN, Lumír KREJČÍ, Jean-Francois CONSTANT a Muriel JOURDAN. Structure and Dynamics of DNA Duplexes Containing a Cluster of Mutagenic 8-Oxoguanine and Abasic Site Lesions. Journal of Molecular Biology, London: Academic Press, 2014, roč. 426, č. 7, s. 1524-1538. ISSN 0022-2836. doi:10.1016/j.jmb.2013.12.022.
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Základní údaje
Originální název Structure and Dynamics of DNA Duplexes Containing a Cluster of Mutagenic 8-Oxoguanine and Abasic Site Lesions
Autoři ZÁLEŠÁK, Jan (203 Česká republika), Morgane LOURDIN (250 Francie), Lumír KREJČÍ (203 Česká republika, garant, domácí), Jean-Francois CONSTANT (250 Francie) a Muriel JOURDAN (250 Francie).
Vydání Journal of Molecular Biology, London, Academic Press, 2014, 0022-2836.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 1.6 Biological sciences
Stát vydavatele Spojené království
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 4.333
Kód RIV RIV/00216224:14110/14:00078633
Organizační jednotka Lékařská fakulta
Doi http://dx.doi.org/10.1016/j.jmb.2013.12.022
UT WoS 000334478000015
Klíčová slova anglicky DNA lesion; NMR; abasic site; 8-oxoguanine; Fpg
Štítky EL OK
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Ing. Mgr. Věra Pospíšilíková, učo 9005. Změněno: 13. 2. 2015 10:57.
Anotace
Clustered DNA damage sites are caused by ionizing radiation. They are much more difficult to repair than are isolated single lesions, and their biological outcomes in terms of mutagenesis and repair inhibition are strongly dependent on the type, relative position and orientation of the lesions present in the cluster. To determine whether these effects on repair mechanism could be due to local structural properties within DNA, we used H-1 NMR spectroscopy and restrained molecular dynamics simulation to elucidate the structures of three DNA duplexes containing bistranded clusters of lesions. Each DNA sequence contained an abasic site in the middle of one strand and differed by the relative position of the 8-oxoguanine, staggered on either the 3' or the 5' side of the complementary strand. Their repair by base excision repair protein Fpg was either complete or inhibited. All the studied damaged DNA duplexes adopt an overall B-form conformation and the damaged residues remain intrahelical. No striking deformations of the DNA chain have been observed as a result of close proximity of the lesions. These results rule out the possibility that differential recognition of clustered DNA lesions by the Fpg protein could be due to changes in the DNA's structural features induced by those lesions and provide new insight into the Fpg recognition process.
VytisknoutZobrazeno: 17. 10. 2019 09:04