Selective In Vitro Anticancer Effect of Superparamagnetic Iron
Oxide Nanoparticles Loaded in Hyaluronan Polymeric Micelles

J 2014

Selective In Vitro Anticancer Effect of Superparamagnetic Iron Oxide Nanoparticles Loaded in Hyaluronan Polymeric Micelles

ŠMEJKALOVÁ, Daniela; Kristina NEŠPOROVÁ; Gloria HUERTA-ANGELES; Jakub SYROVÁTKA; Daniel JIRÁK et al.

Základní údaje

Originální název

Selective In Vitro Anticancer Effect of Superparamagnetic Iron Oxide Nanoparticles Loaded in Hyaluronan Polymeric Micelles

Název anglicky

Selective In Vitro Anticancer Effect of Superparamagnetic Iron Oxide Nanoparticles Loaded in Hyaluronan Polymeric Micelles

Autoři

ŠMEJKALOVÁ, Daniela; Kristina NEŠPOROVÁ; Gloria HUERTA-ANGELES; Jakub SYROVÁTKA; Daniel JIRÁK; Andrea GÁLISOVÁ a Vladimír VELEBNÝ

Vydání

Biomacromolecules, 2014

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

DOI

https://doi.org/10.1021/bm501065q

UT WoS

000344721400015

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 2. 2015 17:05, Mgr. Kristina Nešporová, Ph.D.

Anotace

V originále

Due to its native origin, excellent biocompatibility and biodegradability, hyaluronan (HA) represents an attractive polymer for superparamagnetic iron oxide nanoparticles (SPION) coating. Herein, we report HA polymeric micelles encapsulating oleic acid coated SPIONs, having a hydrodynamic size of about 100 nm and SPION loading capacity of 1–2 wt %. The HA-SPION polymeric micelles were found to be selectively cytotoxic toward a number of human cancer cell lines, mainly those of colon adenocarcinoma (HT-29). The selective inhibition of cell growth was even observed when the SPION loaded HA polymeric micelles were incubated with a mixture of control and cancer cells. The selective in vitro inhibition could not be connected with an enhanced CD44 uptake or radical oxygen species formation and was rather connected with a different way of SPION intracellular release. While aggregated iron particles were visualized in control cells, nonaggregated solubilized iron oxide particles were detected in cancer cells. In vivo SPION accumulation in intramuscular tumor following an intravenous micelle administration was confirmed by magnetic resonance (MR) imaging and histological analysis. Having a suitable hydrodynamic size, high magnetic relaxivity, and being cancer specific and able to accumulate in vivo in tumors, SPION-loaded HA micelles represent a promising platform for theranostic applications.

Citovat

ŠMEJKALOVÁ, Daniela; Kristina NEŠPOROVÁ; Gloria HUERTA-ANGELES; Jakub SYROVÁTKA; Daniel JIRÁK; Andrea GÁLISOVÁ a Vladimír VELEBNÝ. Selective In Vitro Anticancer Effect of Superparamagnetic Iron Oxide Nanoparticles Loaded in Hyaluronan Polymeric Micelles. Biomacromolecules. 2014, roč. 15, č. 11, s. 4012-4020. Dostupné z: https://doi.org/10.1021/bm501065q.

@article{1220465,
   author = {Šmejkalová, Daniela and Nešporová, Kristina and HuertaandAngeles, Gloria and Syrovátka, Jakub and Jirák, Daniel and Gálisová, Andrea and Velebný, Vladimír},
   article_number = {11},
   doi = {https://doi.org/10.1021/bm501065q},
   journal = {Biomacromolecules},
   title = {Selective In Vitro Anticancer Effect of Superparamagnetic Iron Oxide Nanoparticles Loaded in Hyaluronan Polymeric Micelles},
   volume = {15},
   year = {2014}
}

TY  - JOUR
ID  - 1220465
AU  - Šmejkalová, Daniela - Nešporová, Kristina - Huerta-Angeles, Gloria - Syrovátka, Jakub - Jirák, Daniel - Gálisová, Andrea - Velebný, Vladimír
PY  - 2014
TI  - Selective In Vitro Anticancer Effect of Superparamagnetic Iron Oxide Nanoparticles Loaded in Hyaluronan Polymeric Micelles
JF  - Biomacromolecules
VL  - 15
IS  - 11
SP  - 4012-4020
EP  - 4012-4020
N2  - Due to its native origin, excellent biocompatibility and biodegradability, hyaluronan (HA) represents an attractive polymer for superparamagnetic iron oxide nanoparticles (SPION) coating. Herein, we report HA polymeric micelles encapsulating oleic acid coated SPIONs, having a hydrodynamic size of about 100 nm and SPION loading capacity of 1–2 wt %. The HA-SPION polymeric micelles were found to be selectively cytotoxic toward a number of human cancer cell lines, mainly those of colon adenocarcinoma (HT-29). The selective inhibition of cell growth was even observed when the SPION loaded HA polymeric micelles were incubated with a mixture of control and cancer cells. The selective in vitro inhibition could not be connected with an enhanced CD44 uptake or radical oxygen species formation and was rather connected with a different way of SPION intracellular release. While aggregated iron particles were visualized in control cells, nonaggregated solubilized iron oxide particles were detected in cancer cells. In vivo SPION accumulation in intramuscular tumor following an intravenous micelle administration was confirmed by magnetic resonance (MR) imaging and histological analysis. Having a suitable hydrodynamic size, high magnetic relaxivity, and being cancer specific and able to accumulate in vivo in tumors, SPION-loaded HA micelles represent a promising platform for theranostic applications.
ER  -

ŠMEJKALOVÁ, Daniela; Kristina NEŠPOROVÁ; Gloria HUERTA-ANGELES; Jakub SYROVÁTKA; Daniel JIRÁK; Andrea GÁLISOVÁ a Vladimír VELEBNÝ. Selective In Vitro Anticancer Effect of Superparamagnetic Iron Oxide Nanoparticles Loaded in Hyaluronan Polymeric Micelles. \textit{Biomacromolecules}. 2014, roč.~15, č.~11, s.~4012-4020. Dostupné z: https://doi.org/10.1021/bm501065q.

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