2015
Genotyping and virulence factors of Listeria monocytogenes in terms of food safety
GELBÍČOVÁ, Tereza, Ivana KOLÁČKOVÁ a Renata KARPÍŠKOVÁZákladní údaje
Originální název
Genotyping and virulence factors of Listeria monocytogenes in terms of food safety
Autoři
GELBÍČOVÁ, Tereza (203 Česká republika, garant, domácí), Ivana KOLÁČKOVÁ (203 Česká republika) a Renata KARPÍŠKOVÁ (203 Česká republika)
Vydání
Journal of Food and Nutrition Research, 2015, 1336-8672
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Slovensko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 1.676
Kód RIV
RIV/00216224:14310/15:00082400
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000351255700009
Klíčová slova anglicky
food; virulence; internalin A; premature stop codon
Změněno: 27. 10. 2022 13:21, Mgr. Tereza Miškechová
Anotace
V originále
The present study was designed to assess heterogeneity of virulence factors among strains of Listeria monocytogenes isolated from the food chain and humans in the Czech Republic. The virulence characteristics, presence of Listeria pathogenicity island 1 (LIPI-1) as well as inlA, inlB, inlC and inlJ genes of tested strains of L. monocytogenes from foods and food processing plants were comparable with human strains independently of particular serotypes. Restriction polymorphism of inlA gene confirmed a correlation between the restriction fragment length polymorphism (RFLP) profiles and the serotypes. Strains of serotype 1/2a and 1/2c with RFLP profiles, which may be characterized by production of a truncated internalin A, were detected not only in food strains, but also in 44% of strains isolated from clinical cases of listeriosis. Premature stop codon (PMSC mutation type 3) in the inlA gene associated with the production of truncated internalin A was detected in one L. monocytogenes strain from a ready-to-eat fish product. Considering food safety, all tested food strains should still be considered as pathogenic to humans even though some of these showed reduced virulence on the basis of genotyping results.
Návaznosti
EE2.3.20.0183, projekt VaV |
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