Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for
Electrochemical Detection of DNA

J 2014

Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA

SIMONOVA, Anna; Jarmila BÁLINTOVÁ; Radek POHL; Luděk HAVRAN; Miroslav FOJTA et al.

Základní údaje

Originální název

Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA

Autoři

SIMONOVA, Anna; Jarmila BÁLINTOVÁ; Radek POHL; Luděk HAVRAN; Miroslav FOJTA a Michal HOCEK

Vydání

CHEMPLUSCHEM, Weinheim, Wiley - Verlag Chemie, 2014, 2192-6506

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.026

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/14:00074406

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

DNA; electrochemistry; nucleotides; polymerase chain reactions; redox labeling

Štítky

kontrola MP, MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 2. 2015 14:36, Martina Prášilová

Anotace

V originále

4-Hydroxy-3-methoxyphenyl (MOP) and 2,3-dihydrobenzofuran-5-yl (DHB) groups were tested as potential new oxidizable labels for electrochemical detection of DNA. The corresponding 5-aryl-cytosine and 7-aryl-7-deazaadenine 2-deoxyribonucleoside triphosphates (dNTPs) were prepared by the Suzuki coupling of halogenated dNTPs or by triphosphorylation of modified nucleosides, and were incorporated enzymatically into DNA by polymerase. In parallel, the electrochemical behavior of the labeled nucleosides, nucleotides, and DNA was studied to show that most of them give analytically useful signals of oxidation on the carbon electrode. Particularly, MOP appears to be a useful oxidizable DNA label since it offers independent detection of the modified DNA.

Návaznosti

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

SIMONOVA, Anna; Jarmila BÁLINTOVÁ; Radek POHL; Luděk HAVRAN; Miroslav FOJTA a Michal HOCEK. Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA. CHEMPLUSCHEM. Weinheim: Wiley - Verlag Chemie, 2014, roč. 79, č. 12, s. 1703-1712. ISSN 2192-6506. Dostupné z: https://doi.org/10.1002/cplu.201402194.

@article{1227612,
   author = {Simonova, Anna and Bálintová, Jarmila and Pohl, Radek and Havran, Luděk and Fojta, Miroslav and Hocek, Michal},
   article_location = {Weinheim},
   article_number = {12},
   doi = {https://doi.org/10.1002/cplu.201402194},
   keywords = {DNA; electrochemistry; nucleotides; polymerase chain reactions; redox labeling},
   language = {eng},
   issn = {2192-6506},
   journal = {CHEMPLUSCHEM},
   title = {Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/cplu.201402194/full},
   volume = {79},
   year = {2014}
}

TY  - JOUR
ID  - 1227612
AU  - Simonova, Anna - Bálintová, Jarmila - Pohl, Radek - Havran, Luděk - Fojta, Miroslav - Hocek, Michal
PY  - 2014
TI  - Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA
JF  - CHEMPLUSCHEM
VL  - 79
IS  - 12
SP  - 1703-1712
EP  - 1703-1712
PB  - Wiley - Verlag Chemie
SN  - 21926506
KW  - DNA
KW  - electrochemistry
KW  - nucleotides
KW  - polymerase chain reactions
KW  - redox labeling
UR  - http://onlinelibrary.wiley.com/doi/10.1002/cplu.201402194/full
L2  - http://onlinelibrary.wiley.com/doi/10.1002/cplu.201402194/full
N2  - 4-Hydroxy-3-methoxyphenyl (MOP) and 2,3-dihydrobenzofuran-5-yl (DHB) groups were tested as potential new oxidizable labels for electrochemical detection of DNA. The corresponding 5-aryl-cytosine and 7-aryl-7-deazaadenine 2-deoxyribonucleoside triphosphates (dNTPs) were prepared by the Suzuki coupling of halogenated dNTPs or by triphosphorylation of modified nucleosides, and were incorporated enzymatically into DNA by polymerase. In parallel, the electrochemical behavior of the labeled nucleosides, nucleotides, and DNA was studied to show that most of them give analytically useful signals of oxidation on the carbon electrode. Particularly, MOP appears to be a useful oxidizable DNA label since it offers independent detection of the modified DNA.
ER  -

SIMONOVA, Anna; Jarmila BÁLINTOVÁ; Radek POHL; Luděk HAVRAN; Miroslav FOJTA a Michal HOCEK. Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA. \textit{CHEMPLUSCHEM}. Weinheim: Wiley - Verlag Chemie, 2014, roč.~79, č.~12, s.~1703-1712. ISSN~2192-6506. Dostupné z: https://doi.org/10.1002/cplu.201402194.

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