Dynamic infrared imaging analysis of apical hook development in
Arabidopsis: the case of brassinosteroids

J 2014

Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids

SMET, D., P. ŽÁDNÍKOVÁ, F. VANDENBUSSCHE, Eva FRIMLOVÁ, D. VAN DER STRAETEN et. al.

Základní údaje

Originální název

Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids

Autoři

SMET, D. (56 Belgie), P. ŽÁDNÍKOVÁ (56 Belgie), F. VANDENBUSSCHE (56 Belgie), Eva FRIMLOVÁ (703 Slovensko, garant, domácí) a D. VAN DER STRAETEN (56 Belgie)

Vydání

New Phytologist, HOBOKEN, WILEY-BLACKWELL, 2014, 0028-646X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.672

Kód RIV

RIV/00216224:14740/14:00079200

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1111/nph.12751

UT WoS

000335470200029

Klíčová slova anglicky

apical hook; Arabidopsis thaliana; brassinosteroids (BRs); cross-talk; ethylene; infrared imaging; kinematics; light

Štítky

kontrola MP, MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 2. 2015 11:29, Martina Prášilová

Anotace

V originále

Germination of Arabidopsis seeds in darkness induces apical hook development, based on a tightly regulated differential growth coordinated by a multiple hormone cross-talk. Here, we endeavoured to clarify the function of brassinosteroids (BRs) and cross-talk with ethylene in hook development. An automated infrared imaging system was developed to study the kinetics of hook development in etiolated Arabidopsis seedlings. To ascertain the photomorphogenic control of hook opening, the system was equipped with an automatic light dimmer. We demonstrate that ethylene and BRs are indispensable for hook formation and maintenance. Ethylene regulation of hook formation functions partly through BRs, with BR feedback inhibition of ethylene action. Conversely, BR-mediated extension of hook maintenance functions partly through ethylene. Furthermore, we revealed that a short light pulse is sufficient to induce rapid hook opening. Our dynamic infrared imaging system allows high-resolution, kinetic imaging of up to 112 seedlings in a single experimental run. At this high throughput, it is ideally suited to rapidly gain insight in pathway networks. We demonstrate that BRs and ethylene cooperatively regulate apical hook development in a phase-dependent manner. Furthermore, we show that light is a predominant regulator of hook opening, inhibiting ethylene- and BR-mediated postponement of hook opening.

Návaznosti

EE2.3.20.0043, projekt VaV

Název: Rozvoj lidských zdrojů pro výzkum, vývoj a inovace v oblasti genomiky a proteomiky rostlinných systémů

Citovat

SMET, D., P. ŽÁDNÍKOVÁ, F. VANDENBUSSCHE, Eva FRIMLOVÁ a D. VAN DER STRAETEN. Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids. New Phytologist. HOBOKEN: WILEY-BLACKWELL, 2014, roč. 202, č. 4, s. 1398-1411. ISSN 0028-646X. Dostupné z: https://dx.doi.org/10.1111/nph.12751.

@article{1227690,
   author = {Smet, D. and Žádníková, P. and Vandenbussche, F. and Frimlová, Eva and Van Der Straeten, D.},
   article_location = {HOBOKEN},
   article_number = {4},
   doi = {http://dx.doi.org/10.1111/nph.12751},
   keywords = {apical hook; Arabidopsis thaliana; brassinosteroids (BRs); cross-talk; ethylene; infrared imaging; kinematics; light},
   language = {eng},
   issn = {0028-646X},
   journal = {New Phytologist},
   title = {Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/nph.12751/epdf},
   volume = {202},
   year = {2014}
}

TY  - JOUR
ID  - 1227690
AU  - Smet, D. - Žádníková, P. - Vandenbussche, F. - Frimlová, Eva - Van Der Straeten, D.
PY  - 2014
TI  - Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids
JF  - New Phytologist
VL  - 202
IS  - 4
SP  - 1398-1411
EP  - 1398-1411
PB  - WILEY-BLACKWELL
SN  - 0028646X
KW  - apical hook
KW  - Arabidopsis thaliana
KW  - brassinosteroids (BRs)
KW  - cross-talk
KW  - ethylene
KW  - infrared imaging
KW  - kinematics
KW  - light
UR  - http://onlinelibrary.wiley.com/doi/10.1111/nph.12751/epdf
L2  - http://onlinelibrary.wiley.com/doi/10.1111/nph.12751/epdf
N2  - Germination of Arabidopsis seeds in darkness induces apical hook development, based on a tightly regulated differential growth coordinated by a multiple hormone cross-talk. Here, we endeavoured to clarify the function of brassinosteroids (BRs) and cross-talk with ethylene in hook development. An automated infrared imaging system was developed to study the kinetics of hook development in etiolated Arabidopsis seedlings. To ascertain the photomorphogenic control of hook opening, the system was equipped with an automatic light dimmer. We demonstrate that ethylene and BRs are indispensable for hook formation and maintenance. Ethylene regulation of hook formation functions partly through BRs, with BR feedback inhibition of ethylene action. Conversely, BR-mediated extension of hook maintenance functions partly through ethylene. Furthermore, we revealed that a short light pulse is sufficient to induce rapid hook opening. Our dynamic infrared imaging system allows high-resolution, kinetic imaging of up to 112 seedlings in a single experimental run. At this high throughput, it is ideally suited to rapidly gain insight in pathway networks. We demonstrate that BRs and ethylene cooperatively regulate apical hook development in a phase-dependent manner. Furthermore, we show that light is a predominant regulator of hook opening, inhibiting ethylene- and BR-mediated postponement of hook opening.
ER  -

SMET, D., P. ŽÁDNÍKOVÁ, F. VANDENBUSSCHE, Eva FRIMLOVÁ a D. VAN DER STRAETEN. Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids. \textit{New Phytologist}. HOBOKEN: WILEY-BLACKWELL, 2014, roč.~202, č.~4, s.~1398-1411. ISSN~0028-646X. Dostupné z: https://dx.doi.org/10.1111/nph.12751.

Podrobný výpis o publikaci