A simple microviscometric approach based on Brownian motion
tracking

J 2015

A simple microviscometric approach based on Brownian motion tracking

HNYLUCHOVÁ, Zuzana; Petra BJALONČÍKOVÁ; Pavel KARAS; Filip MRAVEC; Tereza HALÁSOVÁ et. al.

Základní údaje

Originální název

A simple microviscometric approach based on Brownian motion tracking

Autoři

HNYLUCHOVÁ, Zuzana (203 Česká republika, garant); Petra BJALONČÍKOVÁ (203 Česká republika); Pavel KARAS (203 Česká republika, domácí); Filip MRAVEC (203 Česká republika); Tereza HALÁSOVÁ (203 Česká republika); Miloslav PEKAŘ (203 Česká republika); Lukáš KUBALA (203 Česká republika) a Jan VÍTEČEK (203 Česká republika)

Vydání

Review of Scientific Instruments, Melville, USA, American Institute of Physics, 2015, 0034-6748

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.336

Kód RIV

RIV/00216224:14330/15:00082430

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.1063/1.4913386

UT WoS

000350552700039

EID Scopus

2-s2.0-84923869849

Klíčová slova anglicky

Viscosity; Brownian motion; Computer software; Diffusion; Particle trajectory

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 5. 2017 06:14, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

Viscosity—an integral property of a liquid—is traditionally determined by mechanical instruments. The most pronounced disadvantage of such an approach is the requirement of a large sample volume, which poses a serious obstacle, particularly in biology and biophysics when working with limited samples. Scaling down the required volume by means of microviscometry based on tracking the Brownian motion of particles can provide a reasonable alternative. In this paper, we report a simple microviscometric approach which can be conducted with common laboratory equipment. The core of this approach consists in a freely available standalone script to process particle trajectory data based on a Newtonian model. In our study, this setup allowed the sample to be scaled down to 10 ul. The utility of the approach was demonstrated using model solutions of glycerine, hyaluronate, and mouse blood plasma. Therefore, this microviscometric approach based on a newly developed freely available script can be suggested for determination of the viscosity of small biological samples (e.g., body fluids).

Citovat

HNYLUCHOVÁ, Zuzana; Petra BJALONČÍKOVÁ; Pavel KARAS; Filip MRAVEC; Tereza HALÁSOVÁ; Miloslav PEKAŘ; Lukáš KUBALA a Jan VÍTEČEK. A simple microviscometric approach based on Brownian motion tracking. Review of Scientific Instruments. Melville, USA: American Institute of Physics, 2015, roč. 86, č. 2, s. 1-5. ISSN 0034-6748. Dostupné z: https://dx.doi.org/10.1063/1.4913386.

@article{1227935,
   author = {Hnyluchová, Zuzana and Bjalončíková, Petra and Karas, Pavel and Mravec, Filip and Halásová, Tereza and Pekař, Miloslav and Kubala, Lukáš and Víteček, Jan},
   article_location = {Melville, USA},
   article_number = {2},
   doi = {http://dx.doi.org/10.1063/1.4913386},
   keywords = {Viscosity; Brownian motion; Computer software; Diffusion; Particle trajectory},
   language = {eng},
   issn = {0034-6748},
   journal = {Review of Scientific Instruments},
   title = {A simple microviscometric approach based on Brownian motion tracking},
   url = {http://scitation.aip.org/content/aip/journal/rsi/86/2/10.1063/1.4913386},
   volume = {86},
   year = {2015}
}

TY  - JOUR
ID  - 1227935
AU  - Hnyluchová, Zuzana - Bjalončíková, Petra - Karas, Pavel - Mravec, Filip - Halásová, Tereza - Pekař, Miloslav - Kubala, Lukáš - Víteček, Jan
PY  - 2015
TI  - A simple microviscometric approach based on Brownian motion tracking
JF  - Review of Scientific Instruments
VL  - 86
IS  - 2
SP  - 1-5
EP  - 1-5
PB  - American Institute of Physics
SN  - 00346748
KW  - Viscosity
KW  - Brownian motion
KW  - Computer software
KW  - Diffusion
KW  - Particle trajectory
UR  - http://scitation.aip.org/content/aip/journal/rsi/86/2/10.1063/1.4913386
N2  - Viscosity—an integral property of a liquid—is traditionally determined by mechanical instruments. The most pronounced disadvantage of such an approach is the requirement of a large sample volume, which poses a serious obstacle, particularly in biology and biophysics when working with limited samples. Scaling down the required volume by means of microviscometry based on tracking the Brownian motion of particles can provide a reasonable alternative. In this paper, we report a simple microviscometric approach which can be conducted with common laboratory equipment. The core of this approach consists in a freely available standalone script to process particle trajectory data based on a Newtonian model. In our study, this setup allowed the sample to be scaled down to 10 ul. The utility of the approach was demonstrated using model solutions of glycerine, hyaluronate, and mouse blood plasma. Therefore, this microviscometric approach based on a newly developed freely available script can be suggested for determination of the viscosity of small biological samples (e.g., body fluids).
ER  -

HNYLUCHOVÁ, Zuzana; Petra BJALONČÍKOVÁ; Pavel KARAS; Filip MRAVEC; Tereza HALÁSOVÁ; Miloslav PEKAŘ; Lukáš KUBALA a Jan VÍTEČEK. A simple microviscometric approach based on Brownian motion tracking. \textit{Review of Scientific Instruments}. Melville, USA: American Institute of Physics, 2015, roč.~86, č.~2, s.~1-5. ISSN~0034-6748. Dostupné z: https://dx.doi.org/10.1063/1.4913386.

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