Age-Related Decrease in TCR Repertoire Diversity Measured with
Deep and Normalized Sequence Profiling

J 2014

Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling

BRITANOVA, O.V., E.V. PUTINTSEVA, M. SHUGAY, E.M. MERZLYAK, M.A. TURCHANINOVA et. al.

Základní údaje

Originální název

Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling

Autoři

BRITANOVA, O.V. (643 Rusko), E.V. PUTINTSEVA (643 Rusko), M. SHUGAY (643 Rusko), E.M. MERZLYAK (643 Rusko), M.A. TURCHANINOVA (643 Rusko), D.B. STAROVEROV (643 Rusko), D.A. BOLOTIN (643 Rusko), S. LUKYANOV (643 Rusko), E.A. BOGDANOVA (643 Rusko), I.Z. MAMEDOV (643 Rusko), Y.B. LEBEDEV (643 Rusko) a Dmitriy CHUDAKOV (643 Rusko, garant, domácí)

Vydání

Journal of immunology, BETHESDA, Williams & Wilkins, 2014, 0022-1767

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30202 Endocrinology and metabolism

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.922

Kód RIV

RIV/00216224:14740/14:00079244

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.4049/jimmunol.1302064

UT WoS

000332702700018

Klíčová slova anglicky

T-CELL REPERTOIRE; RECEPTOR DIVERSITY; THYMIC INVOLUTION; IMMUNE DEFENSE; OLD PRIMATES; IFN-GAMMA; TNF-ALPHA; RESPONSES; HOMEOSTASIS; GENERATION

Štítky

kontrola MP, MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 2. 2015 10:27, Martina Prášilová

Anotace

V originále

The decrease of TCR diversity with aging has never been studied by direct methods. In this study, we combined high-throughput Illumina sequencing with unique cDNA molecular identifier technology to achieve deep and precisely normalized profiling of TCR beta repertoires in 39 healthy donors aged 6-90 y. We demonstrate that TCR beta diversity per 10(6) T cells decreases roughly linearly with age, with significant reduction already apparent by age 40. The percentage of naive T cells showed a strong correlation with measured TCR diversity and decreased linearly up to age 70. Remarkably, the oldest group (average age 82 y) was characterized by a higher percentage of naive CD4(+) T cells, lower abundance of expanded clones, and increased TCR diversity compared with the previous age group (average age 62 y), suggesting the influence of age selection and association of these three related parameters with longevity. Interestingly, cross-analysis of individual TCR beta repertoires revealed a set >10,000 of the most representative public TCR beta clonotypes, whose abundance among the top 100,000 clones correlated with TCR diversity and decreased with aging.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

BRITANOVA, O.V., E.V. PUTINTSEVA, M. SHUGAY, E.M. MERZLYAK, M.A. TURCHANINOVA, D.B. STAROVEROV, D.A. BOLOTIN, S. LUKYANOV, E.A. BOGDANOVA, I.Z. MAMEDOV, Y.B. LEBEDEV a Dmitriy CHUDAKOV. Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling. Journal of immunology. BETHESDA: Williams & Wilkins, 2014, roč. 192, č. 6, s. 2689-2698. ISSN 0022-1767. Dostupné z: https://dx.doi.org/10.4049/jimmunol.1302064.

@article{1228049,
   author = {Britanova, O.V. and Putintseva, E.V. and Shugay, M. and Merzlyak, E.M. and Turchaninova, M.A. and Staroverov, D.B. and Bolotin, D.A. and Lukyanov, S. and Bogdanova, E.A. and Mamedov, I.Z. and Lebedev, Y.B. and Chudakov, Dmitriy},
   article_location = {BETHESDA},
   article_number = {6},
   doi = {http://dx.doi.org/10.4049/jimmunol.1302064},
   keywords = {T-CELL REPERTOIRE; RECEPTOR DIVERSITY; THYMIC INVOLUTION; IMMUNE DEFENSE; OLD PRIMATES; IFN-GAMMA; TNF-ALPHA; RESPONSES; HOMEOSTASIS; GENERATION},
   language = {eng},
   issn = {0022-1767},
   journal = {Journal of immunology},
   title = {Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling},
   volume = {192},
   year = {2014}
}

TY  - JOUR
ID  - 1228049
AU  - Britanova, O.V. - Putintseva, E.V. - Shugay, M. - Merzlyak, E.M. - Turchaninova, M.A. - Staroverov, D.B. - Bolotin, D.A. - Lukyanov, S. - Bogdanova, E.A. - Mamedov, I.Z. - Lebedev, Y.B. - Chudakov, Dmitriy
PY  - 2014
TI  - Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling
JF  - Journal of immunology
VL  - 192
IS  - 6
SP  - 2689-2698
EP  - 2689-2698
PB  - Williams & Wilkins
SN  - 00221767
KW  - T-CELL REPERTOIRE
KW  - RECEPTOR DIVERSITY
KW  - THYMIC INVOLUTION
KW  - IMMUNE DEFENSE
KW  - OLD PRIMATES
KW  - IFN-GAMMA
KW  - TNF-ALPHA
KW  - RESPONSES
KW  - HOMEOSTASIS
KW  - GENERATION
N2  - The decrease of TCR diversity with aging has never been studied by direct methods. In this study, we combined high-throughput Illumina sequencing with unique cDNA molecular identifier technology to achieve deep and precisely normalized profiling of TCR beta repertoires in 39 healthy donors aged 6-90 y. We demonstrate that TCR beta diversity per 10(6) T cells decreases roughly linearly with age, with significant reduction already apparent by age 40. The percentage of naive T cells showed a strong correlation with measured TCR diversity and decreased linearly up to age 70. Remarkably, the oldest group (average age 82 y) was characterized by a higher percentage of naive CD4(+) T cells, lower abundance of expanded clones, and increased TCR diversity compared with the previous age group (average age 62 y), suggesting the influence of age selection and association of these three related parameters with longevity. Interestingly, cross-analysis of individual TCR beta repertoires revealed a set >10,000 of the most representative public TCR beta clonotypes, whose abundance among the top 100,000 clones correlated with TCR diversity and decreased with aging.
ER  -

BRITANOVA, O.V., E.V. PUTINTSEVA, M. SHUGAY, E.M. MERZLYAK, M.A. TURCHANINOVA, D.B. STAROVEROV, D.A. BOLOTIN, S. LUKYANOV, E.A. BOGDANOVA, I.Z. MAMEDOV, Y.B. LEBEDEV a Dmitriy CHUDAKOV. Age-Related Decrease in TCR Repertoire Diversity Measured with Deep and Normalized Sequence Profiling. \textit{Journal of immunology}. BETHESDA: Williams \&{} Wilkins, 2014, roč.~192, č.~6, s.~2689-2698. ISSN~0022-1767. Dostupné z: https://dx.doi.org/10.4049/jimmunol.1302064.

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