The Kjeldahl Method as a Primary Reference Procedure for Total
Protein in Certified Reference Materials Used in Clinical
Chemistry. II. Selection of Direct Kjeldahl Analysis and Its
Preliminary Performance Parameters

J 2015

The Kjeldahl Method as a Primary Reference Procedure for Total Protein in Certified Reference Materials Used in Clinical Chemistry. II. Selection of Direct Kjeldahl Analysis and Its Preliminary Performance Parameters

VINKLÁRKOVÁ, Bára; Vratislav CHROMÝ; Luděk ŠPRONGL; Miroslava BITTOVÁ; Milena RIKANOVÁ et al.

Základní údaje

Originální název

The Kjeldahl Method as a Primary Reference Procedure for Total Protein in Certified Reference Materials Used in Clinical Chemistry. II. Selection of Direct Kjeldahl Analysis and Its Preliminary Performance Parameters

Autoři

VINKLÁRKOVÁ, Bára; Vratislav CHROMÝ; Luděk ŠPRONGL; Miroslava BITTOVÁ; Milena RIKANOVÁ; Ivana OHNÚTKOVÁ a Lenka ŽALUDOVÁ

Vydání

Critical Reviews in Analytical Chemistry, 2015, 1040-8347

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.667

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/15:00082456

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

serum-based reference materials; Kjeldahl method; total protein

Štítky

AKR, rivok

Změněno: 11. 1. 2016 15:56, Mgr. Miroslava Bittová, Ph.D.

Anotace

V originále

To select a Kjeldahl procedure suitable for the determination of total protein in reference materials used in laboratory medicine, we reviewed in our previous article Kjeldahl methods adopted by clinical chemistry and found an indirect two-step analysis by total Kjeldahl nitrogen corrected for its nonprotein nitrogen and a direct analysis made on isolated protein precipitates. In this article, we compare both procedures on various reference materials. An indirect Kjeldahl method gave falsely lower results than a direct analysis. Preliminary performance parameters qualify the direct Kjeldahl analysis as a suitable primary reference procedure for the certification of total protein in reference laboratories.

Návaznosti

MUNI/A/1452/2014, interní kód MU

Název: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2015

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro analýzu biologicky významných látek 2015, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

VINKLÁRKOVÁ, Bára; Vratislav CHROMÝ; Luděk ŠPRONGL; Miroslava BITTOVÁ; Milena RIKANOVÁ; Ivana OHNÚTKOVÁ a Lenka ŽALUDOVÁ. The Kjeldahl Method as a Primary Reference Procedure for Total Protein in Certified Reference Materials Used in Clinical Chemistry. II. Selection of Direct Kjeldahl Analysis and Its Preliminary Performance Parameters. Critical Reviews in Analytical Chemistry. 2015, roč. 45, č. 2, s. 112-118. ISSN 1040-8347. Dostupné z: https://doi.org/10.1080/10408347.2014.892821.

@article{1228710,
   author = {Vinklárková, Bára and Chromý, Vratislav and Šprongl, Luděk and Bittová, Miroslava and Rikanová, Milena and Ohnútková, Ivana and Žaludová, Lenka},
   article_number = {2},
   doi = {https://doi.org/10.1080/10408347.2014.892821},
   keywords = {serum-based reference materials; Kjeldahl method; total protein},
   language = {eng},
   issn = {1040-8347},
   journal = {Critical Reviews in Analytical Chemistry},
   title = {The Kjeldahl Method as a Primary Reference Procedure for Total Protein in Certified Reference Materials Used in Clinical Chemistry. II. Selection of Direct Kjeldahl Analysis and Its Preliminary Performance Parameters},
   url = {http://www.tandfonline.com/doi/full/10.1080/10408347.2014.892821#abstract},
   volume = {45},
   year = {2015}
}

TY  - JOUR
ID  - 1228710
AU  - Vinklárková, Bára - Chromý, Vratislav - Šprongl, Luděk - Bittová, Miroslava - Rikanová, Milena - Ohnútková, Ivana - Žaludová, Lenka
PY  - 2015
TI  - The Kjeldahl Method as a Primary Reference Procedure for Total Protein in Certified Reference Materials Used in Clinical Chemistry. II. Selection of Direct Kjeldahl Analysis and Its Preliminary Performance Parameters
JF  - Critical Reviews in Analytical Chemistry
VL  - 45
IS  - 2
SP  - 112-118
EP  - 112-118
SN  - 10408347
KW  - serum-based reference materials
KW  - Kjeldahl method
KW  - total protein
UR  - http://www.tandfonline.com/doi/full/10.1080/10408347.2014.892821#abstract
L2  - http://www.tandfonline.com/doi/full/10.1080/10408347.2014.892821#abstract
N2  - To select a Kjeldahl procedure suitable for the determination of total protein in reference materials used in laboratory medicine, we reviewed in our previous article Kjeldahl methods adopted by clinical chemistry and found an indirect two-step analysis by total Kjeldahl nitrogen corrected for its nonprotein nitrogen and a direct analysis made on isolated protein precipitates. In this article, we compare both procedures on various reference materials. An indirect Kjeldahl method gave falsely lower results than a direct analysis. Preliminary performance parameters qualify the direct Kjeldahl analysis as a suitable primary reference procedure for the certification of total protein in reference laboratories.
ER  -

VINKLÁRKOVÁ, Bára; Vratislav CHROMÝ; Luděk ŠPRONGL; Miroslava BITTOVÁ; Milena RIKANOVÁ; Ivana OHNÚTKOVÁ a Lenka ŽALUDOVÁ. The Kjeldahl Method as a Primary Reference Procedure for Total Protein in Certified Reference Materials Used in Clinical Chemistry. II. Selection of Direct Kjeldahl Analysis and Its Preliminary Performance Parameters. \textit{Critical Reviews in Analytical Chemistry}. 2015, roč.~45, č.~2, s.~112-118. ISSN~1040-8347. Dostupné z: https://doi.org/10.1080/10408347.2014.892821.

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