Identification of a Novel Retrotransposon with Sex
Chromosome-Specific Distribution in Silene latifolia

J 2014

Identification of a Novel Retrotransposon with Sex Chromosome-Specific Distribution in Silene latifolia

KRÁLOVÁ, Tereza, Radim CEGAN, Zdeněk KUBÁT, Jan VRÁNA, Boris VYSKOT et. al.

Basic information

Original name

Identification of a Novel Retrotransposon with Sex Chromosome-Specific Distribution in Silene latifolia

Authors

KRÁLOVÁ, Tereza (203 Czech Republic), Radim CEGAN (203 Czech Republic), Zdeněk KUBÁT (203 Czech Republic), Jan VRÁNA (203 Czech Republic), Boris VYSKOT (203 Czech Republic), Ivan VOGEL (703 Slovakia, belonging to the institution), Eduard KEJNOVSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Roman HOBZA (203 Czech Republic)

Edition

Cytogenetic and Genome research, Basel, Karger, 2014, 1424-8581

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.561

RIV identification code

RIV/00216224:14740/14:00079375

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1159/000362142

UT WoS

000342614100009

Keywords in English

Microdissection; Sex chromosomes; Silene latifolia (white campion); Retrotransposons

Abstract

V originále

Silene latifolia is a dioecious plant species with chromosomal sex determination. Although the evolution of sex chromosomes in S. latifolia has been the subject of numerous studies, a global view of X chromosome structure in this species is still missing. Here, we combine X chromosome microdissection and BAC library screening to isolate new X chromosome-linked sequences. Out of 8 identified BAC clones, only BAC 86M14 showed an X-preferential signal after FISH experiments. Further analysis revealed the existence of the Athila retroelement which is enriched in the X chromosome and nearly absent in the Y chromosome. Based on previous data, the Athila retroelement belongs to the CL3 group of most repetitive sequences in the S. latifolia genome. Structural, transcriptomics and phylogenetic analyses revealed that Athila CL3 represents an old clade in the Athila lineage. We propose a mechanism responsible for Athila CL3 distribution in the S. latifolia genome. (C) 2014 S. Karger AG, Basel

Citovat

KRÁLOVÁ, Tereza, Radim CEGAN, Zdeněk KUBÁT, Jan VRÁNA, Boris VYSKOT, Ivan VOGEL, Eduard KEJNOVSKÝ and Roman HOBZA. Identification of a Novel Retrotransposon with Sex Chromosome-Specific Distribution in Silene latifolia. Cytogenetic and Genome research. Basel: Karger, 2014, vol. 143, 1-3, p. 87-95. ISSN 1424-8581. Available from: https://dx.doi.org/10.1159/000362142.

@article{1229097,
   author = {Králová, Tereza and Cegan, Radim and Kubát, Zdeněk and Vrána, Jan and Vyskot, Boris and Vogel, Ivan and Kejnovský, Eduard and Hobza, Roman},
   article_location = {Basel},
   article_number = {1-3},
   doi = {http://dx.doi.org/10.1159/000362142},
   keywords = {Microdissection; Sex chromosomes; Silene latifolia (white campion); Retrotransposons},
   language = {eng},
   issn = {1424-8581},
   journal = {Cytogenetic and Genome research},
   title = {Identification of a Novel Retrotransposon with Sex Chromosome-Specific Distribution in Silene latifolia},
   url = {http://www.karger.com/Article/Pdf/362142},
   volume = {143},
   year = {2014}
}

TY  - JOUR
ID  - 1229097
AU  - Králová, Tereza - Cegan, Radim - Kubát, Zdeněk - Vrána, Jan - Vyskot, Boris - Vogel, Ivan - Kejnovský, Eduard - Hobza, Roman
PY  - 2014
TI  - Identification of a Novel Retrotransposon with Sex Chromosome-Specific Distribution in Silene latifolia
JF  - Cytogenetic and Genome research
VL  - 143
IS  - 1-3
SP  - 87-95
EP  - 87-95
PB  - Karger
SN  - 14248581
KW  - Microdissection
KW  - Sex chromosomes
KW  - Silene latifolia (white campion)
KW  - Retrotransposons
UR  - http://www.karger.com/Article/Pdf/362142
L2  - http://www.karger.com/Article/Pdf/362142
N2  - Silene latifolia is a dioecious plant species with chromosomal sex determination. Although the evolution of sex chromosomes in S. latifolia has been the subject of numerous studies, a global view of X chromosome structure in this species is still missing. Here, we combine X chromosome microdissection and BAC library screening to isolate new X chromosome-linked sequences. Out of 8 identified BAC clones, only BAC 86M14 showed an X-preferential signal after FISH experiments. Further analysis revealed the existence of the Athila retroelement which is enriched in the X chromosome and nearly absent in the Y chromosome. Based on previous data, the Athila retroelement belongs to the CL3 group of most repetitive sequences in the S. latifolia genome. Structural, transcriptomics and phylogenetic analyses revealed that Athila CL3 represents an old clade in the Athila lineage. We propose a mechanism responsible for Athila CL3 distribution in the S. latifolia genome. (C) 2014 S. Karger AG, Basel
ER  -

KRÁLOVÁ, Tereza, Radim CEGAN, Zdeněk KUBÁT, Jan VRÁNA, Boris VYSKOT, Ivan VOGEL, Eduard KEJNOVSKÝ and Roman HOBZA. Identification of a Novel Retrotransposon with Sex Chromosome-Specific Distribution in Silene latifolia. \textit{Cytogenetic and Genome research}. Basel: Karger, 2014, vol.~143, 1-3, p.~87-95. ISSN~1424-8581. Available from: https://dx.doi.org/10.1159/000362142.

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