Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes
vexans mosquitoes, Czech Republic

J 2014

Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic

RUDOLF, Ivo; Oldřich SEBESTA; Jan MENDEL; Lenka BETÁŠOVÁ; Eva BOCKOVA et al.

Základní údaje

Originální název

Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic

Autoři

RUDOLF, Ivo; Oldřich SEBESTA; Jan MENDEL; Lenka BETÁŠOVÁ; Eva BOCKOVA; Petra JEDLIČKOVÁ; Kristýna VENCLÍKOVÁ; Hana BLAŽEJOVÁ; Silvie ŠIKUTOVÁ a Zdeněk HUBÁLEK

Vydání

Parasitology Research, NEW YORK, SPRINGER, 2014, 0932-0113

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.098

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/14:00079389

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Aedes vexans; Mosquito vectors; Dirofilaria repens; Dogs; Zoonotic dirofilariosis; Setaria spp.

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 4. 2015 18:29, Ing. Andrea Mikešková

Anotace

V originále

The surveillance of vectors for arthropod-borne pathogens is nowadays an important tool in surveillance programmes throughout Europe. Whereas many studies have been performed to screen arthropods for viruses or bacterial pathogens, only limited information is available concerning the geographical distribution and vector range of pathogenic filariae in Central Europe. To consider the prevalence of filarial parasites in mosquito vectors, we performed a molecular survey of mosquitoes for filarial DNA. Mosquito collection was conducted at six study sites in the South Moravian region (Czech Republic) close to the borders with Slovakia and Austria from 2009 to 2011. Molecular screening of mosquitoes was conducted using conventional PCR with primers designed to amplify the mitochondrial cytochromoxidase subunit I gene as well as the partial 5.8S ribosomal RNA gene. A total of 13,222 mosquitoes belonging to six species were captured and distributed into 237 pools with different numbers of individuals. Overall, four pools were positive for Dirofilaria repens (a minimum infection rate 0.03 %) at two study sites (both natural and urban). Another filarial parasite detected during a study into Aedes vexans mosquitoes revealed the closest homology to Setaria spp. We detected specific D. repens DNA in Ae. vexans mosquitoes for the first time in the Czech Republic and confirmed the circulation of Dirofilaria spp. in a natural focus of infection providing an epidemiological link between autochthonous canine cases and mosquito vectors in the area studied.

Citovat

RUDOLF, Ivo; Oldřich SEBESTA; Jan MENDEL; Lenka BETÁŠOVÁ; Eva BOCKOVA; Petra JEDLIČKOVÁ; Kristýna VENCLÍKOVÁ; Hana BLAŽEJOVÁ; Silvie ŠIKUTOVÁ a Zdeněk HUBÁLEK. Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic. Parasitology Research. NEW YORK: SPRINGER, 2014, roč. 113, č. 12, s. 4663-4667. ISSN 0932-0113. Dostupné z: https://doi.org/10.1007/s00436-014-4191-3.

@article{1229175,
   author = {Rudolf, Ivo and Sebesta, Oldřich and Mendel, Jan and Betášová, Lenka and Bockova, Eva and Jedličková, Petra and Venclíková, Kristýna and Blažejová, Hana and Šikutová, Silvie and Hubálek, Zdeněk},
   article_location = {NEW YORK},
   article_number = {12},
   doi = {https://doi.org/10.1007/s00436-014-4191-3},
   keywords = {Aedes vexans; Mosquito vectors; Dirofilaria repens; Dogs; Zoonotic dirofilariosis; Setaria spp.},
   language = {eng},
   issn = {0932-0113},
   journal = {Parasitology Research},
   title = {Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic},
   volume = {113},
   year = {2014}
}

TY  - JOUR
ID  - 1229175
AU  - Rudolf, Ivo - Sebesta, Oldřich - Mendel, Jan - Betášová, Lenka - Bockova, Eva - Jedličková, Petra - Venclíková, Kristýna - Blažejová, Hana - Šikutová, Silvie - Hubálek, Zdeněk
PY  - 2014
TI  - Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic
JF  - Parasitology Research
VL  - 113
IS  - 12
SP  - 4663-4667
EP  - 4663-4667
PB  - SPRINGER
SN  - 09320113
KW  - Aedes vexans
KW  - Mosquito vectors
KW  - Dirofilaria repens
KW  - Dogs
KW  - Zoonotic dirofilariosis
KW  - Setaria spp.
N2  - The surveillance of vectors for arthropod-borne pathogens is nowadays an important tool in surveillance programmes throughout Europe. Whereas many studies have been performed to screen arthropods for viruses or bacterial pathogens, only limited information is available concerning the geographical distribution and vector range of pathogenic filariae in Central Europe. To consider the prevalence of filarial parasites in mosquito vectors, we performed a molecular survey of mosquitoes for filarial DNA. Mosquito collection was conducted at six study sites in the South Moravian region (Czech Republic) close to the borders with Slovakia and Austria from 2009 to 2011. Molecular screening of mosquitoes was conducted using conventional PCR with primers designed to amplify the mitochondrial cytochromoxidase subunit I gene as well as the partial 5.8S ribosomal RNA gene. A total of 13,222 mosquitoes belonging to six species were captured and distributed into 237 pools with different numbers of individuals. Overall, four pools were positive for Dirofilaria repens (a minimum infection rate 0.03 %) at two study sites (both natural and urban). Another filarial parasite detected during a study into Aedes vexans mosquitoes revealed the closest homology to Setaria spp. We detected specific D. repens DNA in Ae. vexans mosquitoes for the first time in the Czech Republic and confirmed the circulation of Dirofilaria spp. in a natural focus of infection providing an epidemiological link between autochthonous canine cases and mosquito vectors in the area studied.
ER  -

RUDOLF, Ivo; Oldřich SEBESTA; Jan MENDEL; Lenka BETÁŠOVÁ; Eva BOCKOVA; Petra JEDLIČKOVÁ; Kristýna VENCLÍKOVÁ; Hana BLAŽEJOVÁ; Silvie ŠIKUTOVÁ a Zdeněk HUBÁLEK. Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic. \textit{Parasitology Research}. NEW YORK: SPRINGER, 2014, roč.~113, č.~12, s.~4663-4667. ISSN~0932-0113. Dostupné z: https://doi.org/10.1007/s00436-014-4191-3.

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