LC-MS/MS determination of potential endocrine disruptors of
cortico signalling in rivers and wastewaters

J 2014

LC-MS/MS determination of potential endocrine disruptors of cortico signalling in rivers and wastewaters

AMMANN, Adrian A.; Petra MACÍKOVÁ; Ksenia J. GROH; Kristin SCHIRMER; Marc J.F. SUTER et al.

Základní údaje

Originální název

LC-MS/MS determination of potential endocrine disruptors of cortico signalling in rivers and wastewaters

Autoři

AMMANN, Adrian A.; Petra MACÍKOVÁ; Ksenia J. GROH; Kristin SCHIRMER a Marc J.F. SUTER

Vydání

Analytical and Bioanalytical chemistry, HEIDELBERG, SPRINGER HEIDELBERG, 2014, 1618-2642

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.436

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/14:00079393

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Environmental endocrine disruptors; Corticosteroid signalling pathway; Pharmaceuticals; Metabolites; Surface water

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 3. 2015 13:17, Ing. Filip Vaculovič

Anotace

V originále

A targeted analytical method was established to determine a large number of chemicals known to interfere with the gluco- and mineralocorticoid signalling pathway. The analytes comprise 30 glucocorticoids and 9 mineralocorticoids. Ten out of these corticosteroids were primary metabolites. Additionally, 14 nonsteroids were included. These analytes represent a broader range of possible adverse modes of action than previously reported. For the simultaneous determination of these structurally diverse compounds, a single-step multimode solid-phase extraction and pre-concentration was applied. Extracts were separated by a short linear HPLC gradient (20 min) on a core shell RP column (2.7 mu m particle size) and compounds identified and quantified by LC-MS/MS. The method provided excellent retention time reproducibility and detection limits in the low nanograms per litre range. Untreated hospital wastewater, wastewater treatment plant influent, treated effluent and river waters were analysed to demonstrate the applicability of the method. The results show that not all compounds were sufficiently eliminated by the wastewater treatment, resulting in the presence of several steroids (similar to 20 ng/L) and nonsteroids in the final effluent, some of them at high concentrations up to 200 ng/L. Most of the detected mono-hydroxylated steroidal transformation products were found at significantly higher concentrations than their parent compounds. We therefore recommend to include these potentially bioactive metabolites in environmental toxicity assessment.

Citovat

AMMANN, Adrian A.; Petra MACÍKOVÁ; Ksenia J. GROH; Kristin SCHIRMER a Marc J.F. SUTER. LC-MS/MS determination of potential endocrine disruptors of cortico signalling in rivers and wastewaters. Analytical and Bioanalytical chemistry. HEIDELBERG: SPRINGER HEIDELBERG, 2014, roč. 406, č. 29, s. 7653-7665. ISSN 1618-2642. Dostupné z: https://doi.org/10.1007/s00216-014-8206-9.

@article{1229204,
   author = {Ammann, Adrian A. and Macíková, Petra and Groh, Ksenia J. and Schirmer, Kristin and Suter, Marc J.F.},
   article_location = {HEIDELBERG},
   article_number = {29},
   doi = {https://doi.org/10.1007/s00216-014-8206-9},
   keywords = {Environmental endocrine disruptors; Corticosteroid signalling pathway; Pharmaceuticals; Metabolites; Surface water},
   language = {eng},
   issn = {1618-2642},
   journal = {Analytical and Bioanalytical chemistry},
   title = {LC-MS/MS determination of potential endocrine disruptors of cortico signalling in rivers and wastewaters},
   url = {http://link.springer.com/article/10.1007%2Fs00216-014-8206-9},
   volume = {406},
   year = {2014}
}

TY  - JOUR
ID  - 1229204
AU  - Ammann, Adrian A. - Macíková, Petra - Groh, Ksenia J. - Schirmer, Kristin - Suter, Marc J.F.
PY  - 2014
TI  - LC-MS/MS determination of potential endocrine disruptors of cortico signalling in rivers and wastewaters
JF  - Analytical and Bioanalytical chemistry
VL  - 406
IS  - 29
SP  - 7653-7665
EP  - 7653-7665
PB  - SPRINGER HEIDELBERG
SN  - 16182642
KW  - Environmental endocrine disruptors
KW  - Corticosteroid signalling pathway
KW  - Pharmaceuticals
KW  - Metabolites
KW  - Surface water
UR  - http://link.springer.com/article/10.1007%2Fs00216-014-8206-9
L2  - http://link.springer.com/article/10.1007%2Fs00216-014-8206-9
N2  - A targeted analytical method was established to determine a large number of chemicals known to interfere with the gluco- and mineralocorticoid signalling pathway. The analytes comprise 30 glucocorticoids and 9 mineralocorticoids. Ten out of these corticosteroids were primary metabolites. Additionally, 14 nonsteroids were included. These analytes represent a broader range of possible adverse modes of action than previously reported. For the simultaneous determination of these structurally diverse compounds, a single-step multimode solid-phase extraction and pre-concentration was applied. Extracts were separated by a short linear HPLC gradient (20 min) on a core shell RP column (2.7 mu m particle size) and compounds identified and quantified by LC-MS/MS. The method provided excellent retention time reproducibility and detection limits in the low nanograms per litre range. Untreated hospital wastewater, wastewater treatment plant influent, treated effluent and river waters were analysed to demonstrate the applicability of the method. The results show that not all compounds were sufficiently eliminated by the wastewater treatment, resulting in the presence of several steroids (similar to 20 ng/L) and nonsteroids in the final effluent, some of them at high concentrations up to 200 ng/L. Most of the detected mono-hydroxylated steroidal transformation products were found at significantly higher concentrations than their parent compounds. We therefore recommend to include these potentially bioactive metabolites in environmental toxicity assessment.
ER  -

AMMANN, Adrian A.; Petra MACÍKOVÁ; Ksenia J. GROH; Kristin SCHIRMER a Marc J.F. SUTER. LC-MS/MS determination of potential endocrine disruptors of cortico signalling in rivers and wastewaters. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2014, roč.~406, č.~29, s.~7653-7665. ISSN~1618-2642. Dostupné z: https://doi.org/10.1007/s00216-014-8206-9.

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