In vitro selection and characterization of putative probiotics
isolated from the gut of Acipenser baerii (Brandt, 1869)

J 2014

In vitro selection and characterization of putative probiotics isolated from the gut of Acipenser baerii (Brandt, 1869)

GERAYLOU, Zahra; Maarten Pieterjan VANHOVE; Caroline SOUFFREAU; Eugene RURANGWA; Johan BUYSE et al.

Základní údaje

Originální název

In vitro selection and characterization of putative probiotics isolated from the gut of Acipenser baerii (Brandt, 1869)

Autoři

GERAYLOU, Zahra; Maarten Pieterjan VANHOVE; Caroline SOUFFREAU; Eugene RURANGWA; Johan BUYSE a Frans OLLEVIER

Vydání

Aquaculture Research, New Jersey, Wiley, 2014, 1355-557X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 1.376

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1111/j.1365-2109.2012.03232.x

UT WoS

000329472000018

Klíčová slova anglicky

Probiotics; Acipenser baerii; in vitro assays; Lactococcus lactis ssp lactis

Štítky

AKR

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 11. 2019 15:13, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

To select and characterize potential probiotic bacteria from the gut microbiota of Siberian sturgeon (Acipenser baerii), 129 strains isolated from the hindgut were screened for antagonistic activity against five fish pathogens. Ten isolates showed antagonism towards three or more pathogens. Nine of these isolates were Gram-positive, belonging to Lactococcus(seven) and Bacillus(two), and a single strain belonging to the Gram-negative Citrobacter. These inhibitory isolates were identified using genetic, phentotypic and biochemical traits, and further characterized byin vitro tests assessing the adhesion and growth in mucus and resistance to gastric and intestinal fluids. The candidate probiotics were determined to be non-pathogenic through an in vivo study. Based on these assays, Lactococcus lactis ssp. lactis STG45 and STG81 showed the broadest inhibitory potential, a high viability in simulated gastrointestinal juice and the highest adhesion capacity to mucus. They were therefore selected as the most promising candidate probiotics. This is the first study screening probiotics among the gut microflora of Siberian sturgeon.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

GERAYLOU, Zahra; Maarten Pieterjan VANHOVE; Caroline SOUFFREAU; Eugene RURANGWA; Johan BUYSE a Frans OLLEVIER. In vitro selection and characterization of putative probiotics isolated from the gut of Acipenser baerii (Brandt, 1869). Aquaculture Research. New Jersey: Wiley, 2014, roč. 45, č. 2, s. 341-352. ISSN 1355-557X. Dostupné z: https://doi.org/10.1111/j.1365-2109.2012.03232.x.

@article{1230130,
   author = {Geraylou, Zahra and Vanhove, Maarten Pieterjan and Souffreau, Caroline and Rurangwa, Eugene and Buyse, Johan and Ollevier, Frans},
   article_location = {New Jersey},
   article_number = {2},
   doi = {https://doi.org/10.1111/j.1365-2109.2012.03232.x},
   keywords = {Probiotics; Acipenser baerii; in vitro assays; Lactococcus lactis ssp lactis},
   language = {eng},
   issn = {1355-557X},
   journal = {Aquaculture Research},
   title = {In vitro selection and characterization of putative probiotics isolated from the gut of Acipenser baerii (Brandt, 1869)},
   url = {https://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-2109.2012.03232.x},
   volume = {45},
   year = {2014}
}

TY  - JOUR
ID  - 1230130
AU  - Geraylou, Zahra - Vanhove, Maarten Pieterjan - Souffreau, Caroline - Rurangwa, Eugene - Buyse, Johan - Ollevier, Frans
PY  - 2014
TI  - In vitro selection and characterization of putative probiotics isolated from the gut of Acipenser baerii (Brandt, 1869)
JF  - Aquaculture Research
VL  - 45
IS  - 2
SP  - 341-352
EP  - 341-352
PB  - Wiley
SN  - 1355557X
KW  - Probiotics
KW  - Acipenser baerii
KW  - in vitro assays
KW  - Lactococcus lactis ssp lactis
UR  - https://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-2109.2012.03232.x
L2  - https://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-2109.2012.03232.x
N2  - To select and characterize potential probiotic bacteria from the gut microbiota of Siberian sturgeon (Acipenser baerii), 129 strains isolated from the hindgut were screened for antagonistic activity against five fish pathogens. Ten isolates showed antagonism towards three or more pathogens. Nine of these isolates were Gram-positive, belonging to Lactococcus(seven) and Bacillus(two), and a single strain belonging to the Gram-negative Citrobacter. These inhibitory isolates were identified using genetic, phentotypic and biochemical traits, and further characterized byin vitro tests assessing the adhesion and growth in mucus and resistance to gastric and intestinal fluids. The candidate probiotics were determined to be non-pathogenic through an in vivo study. Based on these assays, Lactococcus lactis ssp. lactis STG45 and STG81 showed the broadest inhibitory potential, a high viability in simulated gastrointestinal juice and the highest adhesion capacity to mucus. They were therefore selected as the most promising candidate probiotics. This is the first study screening probiotics among the gut microflora of Siberian sturgeon.
ER  -

GERAYLOU, Zahra; Maarten Pieterjan VANHOVE; Caroline SOUFFREAU; Eugene RURANGWA; Johan BUYSE a Frans OLLEVIER. In vitro selection and characterization of putative probiotics isolated from the gut of Acipenser baerii (Brandt, 1869). \textit{Aquaculture Research}. New Jersey: Wiley, 2014, roč.~45, č.~2, s.~341-352. ISSN~1355-557X. Dostupné z: https://doi.org/10.1111/j.1365-2109.2012.03232.x.

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