RNA-dependent disassembly of nuclear bodies

J 2016

RNA-dependent disassembly of nuclear bodies

MUSINOVA, Yana R., Olga M. LISITSYNA, Dmitry SOROKIN, Eugene A. ARIFULIN, Tatiana A. SMIRNOVA et. al.

Základní údaje

Originální název

RNA-dependent disassembly of nuclear bodies

Autoři

MUSINOVA, Yana R. (643 Rusko), Olga M. LISITSYNA (643 Rusko), Dmitry SOROKIN (643 Rusko, garant, domácí), Eugene A. ARIFULIN (643 Rusko), Tatiana A. SMIRNOVA (643 Rusko), Roman A. ZINOVKIN (643 Rusko), Daria M. POTASHNIKOVA (643 Rusko), Yegor S. VASSETZKY (643 Rusko) a Eugene V. SHEVAL (643 Rusko)

Vydání

Journal of Cell Science, The Company of Biologists Ltd. 2016, 0021-9533

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.431

Kód RIV

RIV/00216224:14330/16:00087720

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.1242/jcs.189142

UT WoS

000391103700008

Klíčová slova anglicky

Nuclear bodies; Nucleolus; Interphase prenucleolar body; pre-rRNA; B23; NPM1; Self-organization

Štítky

CBIA, cbia-web

Změněno: 27. 4. 2017 05:41, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

Nuclear bodies are membraneless organelles that play important roles in genome functioning. A specific type of nuclear bodies known as interphase prenucleolar bodies (iPNBs) are formed in the nucleoplasm after hypotonic stress from partially disassembled nucleoli. iPNBs are then disassembled, and the nucleoli are reformed simultaneously. Here, we show that diffusion of B23 molecules (also known as nucleophosmin, NPM1) from iPNBs, but not fusion of iPNBs with the nucleoli, contributes to the transfer of B23 from iPNBs to the nucleoli. Maturation of pre-ribosomal RNAs (rRNAs) and the subsequent outflow of mature rRNAs from iPNBs led to the disassembly of iPNBs. We found that B23 transfer was dependent on the synthesis of pre-rRNA molecules in nucleoli; these pre-rRNA molecules interacted with B23 and led to its accumulation within nucleoli. The transfer of B23 between iPNBs and nucleoli was accomplished through a nucleoplasmic pool of B23, and increased nucleoplasmic B23 content retarded disassembly, whereas B23 depletion accelerated disassembly. Our results suggest that iPNB disassembly and nucleolus assembly might be coupled through RNA-dependent exchange of nucleolar proteins, creating a highly dynamic system with long-distance correlations between spatially distinct processes.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

MUSINOVA, Yana R., Olga M. LISITSYNA, Dmitry SOROKIN, Eugene A. ARIFULIN, Tatiana A. SMIRNOVA, Roman A. ZINOVKIN, Daria M. POTASHNIKOVA, Yegor S. VASSETZKY a Eugene V. SHEVAL. RNA-dependent disassembly of nuclear bodies. Journal of Cell Science. The Company of Biologists Ltd., 2016, roč. 129, č. 24, s. 4509-4520. ISSN 0021-9533. Dostupné z: https://dx.doi.org/10.1242/jcs.189142.

@article{1232676,
   author = {Musinova, Yana R. and Lisitsyna, Olga M. and Sorokin, Dmitry and Arifulin, Eugene A. and Smirnova, Tatiana A. and Zinovkin, Roman A. and Potashnikova, Daria M. and Vassetzky, Yegor S. and Sheval, Eugene V.},
   article_number = {24},
   doi = {http://dx.doi.org/10.1242/jcs.189142},
   keywords = {Nuclear bodies; Nucleolus; Interphase prenucleolar body; pre-rRNA; B23; NPM1; Self-organization},
   language = {eng},
   issn = {0021-9533},
   journal = {Journal of Cell Science},
   title = {RNA-dependent disassembly of nuclear bodies},
   volume = {129},
   year = {2016}
}

TY  - JOUR
ID  - 1232676
AU  - Musinova, Yana R. - Lisitsyna, Olga M. - Sorokin, Dmitry - Arifulin, Eugene A. - Smirnova, Tatiana A. - Zinovkin, Roman A. - Potashnikova, Daria M. - Vassetzky, Yegor S. - Sheval, Eugene V.
PY  - 2016
TI  - RNA-dependent disassembly of nuclear bodies
JF  - Journal of Cell Science
VL  - 129
IS  - 24
SP  - 4509-4520
EP  - 4509-4520
PB  - The Company of Biologists Ltd.
SN  - 00219533
KW  - Nuclear bodies
KW  - Nucleolus
KW  - Interphase prenucleolar body
KW  - pre-rRNA
KW  - B23
KW  - NPM1
KW  - Self-organization
N2  - Nuclear bodies are membraneless organelles that play important roles in genome functioning. A specific type of nuclear bodies known as interphase prenucleolar bodies (iPNBs) are formed in the nucleoplasm after hypotonic stress from partially disassembled nucleoli. iPNBs are then disassembled, and the nucleoli are reformed simultaneously. Here, we show that diffusion of B23 molecules (also known as nucleophosmin, NPM1) from iPNBs, but not fusion of iPNBs with the nucleoli, contributes to the transfer of B23 from iPNBs to the nucleoli. Maturation of pre-ribosomal RNAs (rRNAs) and the subsequent outflow of mature rRNAs from iPNBs led to the disassembly of iPNBs. We found that B23 transfer was dependent on the synthesis of pre-rRNA molecules in nucleoli; these pre-rRNA molecules interacted with B23 and led to its accumulation within nucleoli. The transfer of B23 between iPNBs and nucleoli was accomplished through a nucleoplasmic pool of B23, and increased nucleoplasmic B23 content retarded disassembly, whereas B23 depletion accelerated disassembly. Our results suggest that iPNB disassembly and nucleolus assembly might be coupled through RNA-dependent exchange of nucleolar proteins, creating a highly dynamic system with long-distance correlations between spatially distinct processes.
ER  -

MUSINOVA, Yana R., Olga M. LISITSYNA, Dmitry SOROKIN, Eugene A. ARIFULIN, Tatiana A. SMIRNOVA, Roman A. ZINOVKIN, Daria M. POTASHNIKOVA, Yegor S. VASSETZKY a Eugene V. SHEVAL. RNA-dependent disassembly of nuclear bodies. \textit{Journal of Cell Science}. The Company of Biologists Ltd., 2016, roč.~129, č.~24, s.~4509-4520. ISSN~0021-9533. Dostupné z: https://dx.doi.org/10.1242/jcs.189142.

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