Pluripotent stem cell lines display variable hematopoietic
developmental potential

a 2015

Pluripotent stem cell lines display variable hematopoietic developmental potential

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ

Základní údaje

Originální název

Pluripotent stem cell lines display variable hematopoietic developmental potential

Autoři

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ

Vydání

2015 EMBL Symposium: Frontiers in Stem Cells & Cancer, Heidelberg, Germany, Mar 29-31, 2015. 2015

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10601 Cell biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Fakulta informatiky

Klíčová slova česky

pluripotentní kmenové buňky; hematopoetický vývoj

Klíčová slova anglicky

pluripotent stem cells; hematopoietic development

Příznaky

Mezinárodní význam

Změněno: 2. 3. 2018 09:59, Mgr. Pavel Šimara, Ph.D.

Anotace

V originále

The clinical application of hematopoietic precursors generated from human pluripotent stem cells (hPSCs) is still limited by low efficient differentiation protocols and functional defects in the derived cells. Moreover, the potential of various hPSCs to differentiate into blood cells has not been examined properly. In this study, protocols for hematopoietic differentiation were applied to available human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines to determine their hematopoietic developmental potential. These protocols included different methods for hPSC cultivation and embryoid bodies (EBs) formation and composition of differentiation media. The efficiency of hematopoietic differentiation was found to be variable among hPSC lines. No protocol optimization enabled generation of CD34+ hematopoietic precursors from available hESC lines CCTL-12 and CCTL-14. On the contrary, these precursors were detected during hiPSC differentiation in basic media supplemented with three cytokines. The yield of precursors was variable, ranging from 0.8 to 10.3 percent of CD34+ cells and from 1.0 to 10,6 percent of CD43+ cells at day seven of differentiation. This yield was further increased when hiPSCs were differentiated for ten days in media with richer cytokine supplement. Nevertheless the variability among hiPSC lines was retained, 6.4 to 16.3 percent of cells were CD34+ and 7.8 to 20.0 percent of cells were CD43+235+ precursors of primitive hematopoiesis, while CD45+ precursors of definitive hematopoiesis comprised only from 0.4 to 4.7 percent. These results indicate that hematopoietic developmental potential of individual hPSC lines is characterised by significant variability that has to be overcome before blood cells derived from hPSC could be used for clinical applications.

Návaznosti

CZ.1.07/2.3.00/30.0030, interní kód MU

Název: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Rozvoj lidských zdrojů pro oblast buněčné biologie, 2.3 Lidské zdroje ve výzkumu a vývoji

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ. Pluripotent stem cell lines display variable hematopoietic developmental potential. In 2015 EMBL Symposium: Frontiers in Stem Cells & Cancer, Heidelberg, Germany, Mar 29-31, 2015. 2015.

@proceedings{1297167,
   author = {Tesařová, Lenka and Šimara, Pavel and Stejskal, Stanislav and Krontorád Koutná, Irena},
   booktitle = {2015 EMBL Symposium: Frontiers in Stem Cells & Cancer, Heidelberg, Germany, Mar 29-31, 2015.},
   keywords = {pluripotent stem cells; hematopoietic development},
   language = {eng},
   title = {Pluripotent stem cell lines display variable hematopoietic developmental potential},
   year = {2015}
}

TY  - CONF
ID  - 1297167
AU  - Tesařová, Lenka - Šimara, Pavel - Stejskal, Stanislav - Krontorád Koutná, Irena
PY  - 2015
TI  - Pluripotent stem cell lines display variable hematopoietic developmental potential
KW  - pluripotent stem cells
KW  - hematopoietic development
N2  - The clinical application of hematopoietic precursors generated from human pluripotent stem cells (hPSCs) is still limited by low efficient differentiation protocols and functional defects in the derived cells. Moreover, the potential of various hPSCs to differentiate into blood cells has not been examined properly. In this study, protocols for hematopoietic differentiation were applied to available human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines to determine their hematopoietic developmental potential. These protocols included different methods for hPSC cultivation and embryoid bodies (EBs) formation and composition of differentiation media. The efficiency of hematopoietic differentiation was found to be variable among hPSC lines. No protocol optimization enabled generation of CD34+ hematopoietic precursors from available hESC lines CCTL-12 and CCTL-14. On the contrary, these precursors were detected during hiPSC differentiation in basic media supplemented with three cytokines. The yield of precursors was variable, ranging from 0.8 to 10.3 percent of CD34+ cells and from 1.0 to 10,6 percent of CD43+ cells at day seven of differentiation. This yield was further increased when hiPSCs were differentiated for ten days in media with richer cytokine supplement. Nevertheless the variability among hiPSC lines was retained, 6.4 to 16.3 percent of cells were CD34+ and 7.8 to 20.0 percent of cells were CD43+235+ precursors of primitive hematopoiesis, while CD45+ precursors of definitive hematopoiesis comprised only from 0.4 to 4.7 percent. These results indicate that hematopoietic developmental potential of individual hPSC lines is characterised by significant variability that has to be overcome before blood cells derived from hPSC could be used for clinical applications.
ER  -

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ. Pluripotent stem cell lines display variable hematopoietic developmental potential. In \textit{2015 EMBL Symposium: Frontiers in Stem Cells \&{} Cancer, Heidelberg, Germany, Mar 29-31, 2015.}. 2015.

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