Endocyted gold-nanoparticles in intact-cell mass spectrometry

a 2015

Endocyted gold-nanoparticles in intact-cell mass spectrometry

FERNANDEZ PEREZ, Raquel; Lenka KOLÁŘOVÁ; Petr VAŇHARA; Lukáš KUČERA; Victoria SALVADO et al.

Základní údaje

Originální název

Endocyted gold-nanoparticles in intact-cell mass spectrometry

Autoři

FERNANDEZ PEREZ, Raquel; Lenka KOLÁŘOVÁ; Petr VAŇHARA; Lukáš KUČERA; Victoria SALVADO; Aleš HAMPL a Josef HAVEL

Vydání

CEITEC PhD Retreat, 23-24 April 2015, Valtice, Czech Republic, 2015. 2015

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

ISBN

978-80-210-7825-3

Klíčová slova anglicky

Mass spectrometry; Gold nanoparticles, Human Embryonic Kidney 293 cells

Změněno: 5. 5. 2015 15:19, Mgr. Lenka Kolářová, Ph.D.

Anotace

V originále

Gold nanoparticles (AuNPs) were recently reported to increase ionization of biomolecules in Matrix-Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) Mass Spectrometry (MS) of complex biological samples, such as cells and tissues.[1-2] In this setup, the matrix-sample mixture is enriched with AuNPs of various size and geometry that most probably enhance distribution of laser energy throughout the sample and facilitate ionization and/or quantum nano-plasmons effects during the process of measurements. We asked, whether AuNPs endocyted by cultured cells can enhance subsequent MS analysis. In this work, we treated HEK293 cells with either polyhedral or flower-like AuNPs or left them untreated and cultured them overnight. Then the cells were washed, harvested, mixed with matrix and directly analyzed by MS. Both the polyhedral and flower-like AuNPs were massively endocyted by HEK293 cells. The mass spectra acquired from intact cells containing endocyted AuNPs showed an increase of intensity of specific peaks. Interestingly, mass spectra generated from intact cells containing polyhedral or flower-like AuNPs showed differences in m/z or intensity values, suggesting selective effect of AuNPs structure on cell targets. The endocyted nanoparticles can interact with various components of cellular metabolism. The interactions of AuNPs with biomolecular pool in living cells can therefore extend the analytical approaches such as MALDI, SALDI (Surface Assisted Laser Desorption Ionization) or NALDI (Nano Particles Assisted Laser Desorption Ionization) used in intact cell MS.

Citovat

FERNANDEZ PEREZ, Raquel; Lenka KOLÁŘOVÁ; Petr VAŇHARA; Lukáš KUČERA; Victoria SALVADO; Aleš HAMPL a Josef HAVEL. Endocyted gold-nanoparticles in intact-cell mass spectrometry. In CEITEC PhD Retreat, 23-24 April 2015, Valtice, Czech Republic, 2015. 2015. ISBN 978-80-210-7825-3.

@proceedings{1298960,
   author = {Fernandez Perez, Raquel and Kolářová, Lenka and Vaňhara, Petr and Kučera, Lukáš and Salvado, Victoria and Hampl, Aleš and Havel, Josef},
   booktitle = {CEITEC PhD Retreat, 23-24 April 2015, Valtice, Czech Republic, 2015.},
   keywords = {Mass spectrometry; Gold nanoparticles, Human Embryonic Kidney 293 cells},
   language = {eng},
   isbn = {978-80-210-7825-3},
   title = {Endocyted gold-nanoparticles in intact-cell mass spectrometry},
   year = {2015}
}

TY  - CONF
ID  - 1298960
AU  - Fernandez Perez, Raquel - Kolářová, Lenka - Vaňhara, Petr - Kučera, Lukáš - Salvado, Victoria - Hampl, Aleš - Havel, Josef
PY  - 2015
TI  - Endocyted gold-nanoparticles in intact-cell mass spectrometry
SN  - 9788021078253
KW  - Mass spectrometry
KW  - Gold nanoparticles, Human Embryonic Kidney 293 cells
N2  - Gold nanoparticles (AuNPs) were recently reported to increase ionization of biomolecules in Matrix-Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) Mass Spectrometry (MS) of complex biological samples, such as cells and tissues.[1-2] In this setup, the matrix-sample mixture is enriched with AuNPs of various size and geometry that most probably enhance distribution of laser energy throughout the sample and facilitate ionization and/or quantum nano-plasmons effects during the process of measurements. We asked, whether AuNPs endocyted by cultured cells can enhance subsequent MS analysis. In this work, we treated HEK293 cells with either polyhedral or flower-like AuNPs or left them untreated and cultured them overnight. Then the cells were washed, harvested, mixed with matrix and directly analyzed by MS. Both the polyhedral and flower-like AuNPs were massively endocyted by HEK293 cells. The mass spectra acquired from intact cells containing endocyted AuNPs showed an increase of intensity of specific peaks. Interestingly, mass spectra generated from intact cells containing polyhedral or flower-like AuNPs showed differences in m/z or intensity values, suggesting selective effect of AuNPs structure on cell targets. The endocyted nanoparticles can interact with various components of cellular metabolism. The interactions of AuNPs with biomolecular pool in living cells can therefore extend the analytical approaches such as MALDI, SALDI (Surface Assisted Laser Desorption Ionization) or NALDI (Nano Particles Assisted Laser Desorption Ionization) used in intact cell MS.
ER  -

FERNANDEZ PEREZ, Raquel; Lenka KOLÁŘOVÁ; Petr VAŇHARA; Lukáš KUČERA; Victoria SALVADO; Aleš HAMPL a Josef HAVEL. Endocyted gold-nanoparticles in intact-cell mass spectrometry. In \textit{CEITEC PhD Retreat, 23-24 April 2015, Valtice, Czech Republic, 2015.}. 2015. ISBN~978-80-210-7825-3.

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