Novel molecular diagnostic approach for detection of pathogens
in meat

k 2015

Novel molecular diagnostic approach for detection of pathogens in meat

RESLOVÁ, Nikol; Martin KAŠNÝ; Michal SLANÝ; Břetislav KOUDELA; Petr KRÁLÍK et al.

Základní údaje

Originální název

Novel molecular diagnostic approach for detection of pathogens in meat

Autoři

RESLOVÁ, Nikol; Martin KAŠNÝ; Michal SLANÝ; Břetislav KOUDELA a Petr KRÁLÍK

Vydání

22nd Helminthological Days, 2015

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/15:00089066

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-6796-7

Klíčová slova anglicky

diagnostics; helminths; MOL-PCR; MAGPIX; meat products; trichinella spp.; anisakis spp.; taenia spp.; diphyllobothrium spp.

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 3. 2018 09:40, Mgr. Lucie Jarošová, DiS.

Anotace

V originále

The changes in farming practices towards bio-production, globalization of food market with increasing animal transportation and also global climate change facilitate the risks of widespread disseminations of food-borne diseases, including the pathogens, such as zoonotic multicellular parasites. These facts consequently induce the need of improvement of the diagnostic tools also for food-borne agents in the final products such as meat. In relation to this problematics, our work is focused on development of reliable comprehensive molecular diagnostic method useful for rapid control of final meat products on the market. For this purpose, we adopted high sensitive multiplex oligonucleotide ligation- PCR assay - MOL-PCR, representing the novel diagnostic platform based on magnetic microspheres and visualization realized via MAGPIX instrument for qualitative and quantitative readout of signal. This modern approach enables simultaneous direct screen of complex samples potentially containing the DNA originating from number of different parasitic organisms. Up to this date, the specific molecular probes allowing the detection and capturing of targeted DNA from two parasitic worms - Trichinella spiralis (partial sequence of 18S rRNA gene) and Taenia saginata (partial sequence of mitochondrial COX1 gene) were designed. The calibration of the system and optimization of method is in process.

Návaznosti

MUNI/A/1484/2014, interní kód MU

Název: Analýzy diverzity biologických systémů různých úrovní a na různých škálách terestrického a akvatického prostředí (Akronym: BIDA4)

Investor: Masarykova univerzita, Analýzy diverzity biologických systémů různých úrovní a na různých škálách terestrického a akvatického prostředí, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

RESLOVÁ, Nikol; Martin KAŠNÝ; Michal SLANÝ; Břetislav KOUDELA a Petr KRÁLÍK. Novel molecular diagnostic approach for detection of pathogens in meat. In 22nd Helminthological Days. 2015. ISBN 978-80-210-6796-7.

@proceedings{1299633,
   author = {Reslová, Nikol and Kašný, Martin and Slaný, Michal and Koudela, Břetislav and Králík, Petr},
   booktitle = {22nd Helminthological Days},
   keywords = {diagnostics; helminths; MOL-PCR; MAGPIX; meat products; trichinella spp.; anisakis spp.; taenia spp.; diphyllobothrium spp.},
   language = {eng},
   isbn = {978-80-210-6796-7},
   title = {Novel molecular diagnostic approach for detection of pathogens in meat},
   year = {2015}
}

TY  - CONF
ID  - 1299633
AU  - Reslová, Nikol - Kašný, Martin - Slaný, Michal - Koudela, Břetislav - Králík, Petr
PY  - 2015
TI  - Novel molecular diagnostic approach for detection of pathogens in meat
SN  - 9788021067967
KW  - diagnostics
KW  - helminths
KW  - MOL-PCR
KW  - MAGPIX
KW  - meat products
KW  - trichinella spp.
KW  - anisakis spp.
KW  - taenia spp.
KW  - diphyllobothrium spp.
N2  - The changes in farming practices towards bio-production, globalization of food market with increasing animal transportation and also global climate change facilitate the risks of widespread disseminations of food-borne diseases, including the pathogens, such as zoonotic multicellular parasites. These facts consequently induce the need of improvement of the diagnostic tools also for food-borne agents in the final products such as meat. In relation to this problematics, our work is focused on development of reliable comprehensive molecular diagnostic method useful for rapid control of final meat products on the market. For this purpose, we adopted high sensitive multiplex oligonucleotide ligation- PCR assay - MOL-PCR, representing the novel diagnostic platform based on magnetic microspheres and visualization realized via MAGPIX instrument for qualitative and quantitative readout of signal. This modern approach enables simultaneous direct screen of complex samples potentially containing the DNA originating from number of different parasitic organisms. Up to this date, the specific molecular probes allowing the detection and capturing of targeted DNA from two parasitic worms - Trichinella spiralis (partial sequence of 18S rRNA gene) and Taenia saginata (partial sequence of mitochondrial COX1 gene) were designed. The calibration of the system and optimization of method is in process.
ER  -

RESLOVÁ, Nikol; Martin KAŠNÝ; Michal SLANÝ; Břetislav KOUDELA a Petr KRÁLÍK. Novel molecular diagnostic approach for detection of pathogens in meat. In \textit{22nd Helminthological Days}. 2015. ISBN~978-80-210-6796-7.

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