The cell adhesion and cytotoxicity effects of the derivate of
vitamin E compared for two cell lines using a piezoelectric
biosensor

J 2012

The cell adhesion and cytotoxicity effects of the derivate of vitamin E compared for two cell lines using a piezoelectric biosensor

FOHLEROVÁ, Zdenka, Jaroslav TURÁNEK a Petr SKLÁDAL

Základní údaje

Originální název

The cell adhesion and cytotoxicity effects of the derivate of vitamin E compared for two cell lines using a piezoelectric biosensor

Autoři

FOHLEROVÁ, Zdenka (203 Česká republika, domácí), Jaroslav TURÁNEK (203 Česká republika) a Petr SKLÁDAL (203 Česká republika, garant, domácí)

Vydání

Sensors and Actuators B Chemical, Amsterdam (Holandsko), Elsevier, 2012, 0925-4005

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.535

Kód RIV

RIV/00216224:14740/12:00080243

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.snb.2012.08.047

UT WoS

000311329000022

Klíčová slova anglicky

Quartz crystal microbalance; Fluorescence microscopy; Cell adhesion; Extracellular matrix; Apoptosis; Vitamin E

Štítky

kontrola MP, MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 5. 2015 10:39, Martina Prášilová

Anotace

V originále

The quartz crystal microbalance (QCM) was used to construct a piezoelectric biosensor utilizing rat liver epithelial cells (WB F344) and lung melanoma cells (B16F10) as biorecognition elements. The cells adhered on the gold surface modified by either hydrophobic polystyrene or fibronectin. The simultaneous monitoring of changes in the resonant frequency Delta f and the resistance Delta R corresponded with the mass and viscoelastic properties of the adhered cells. In addition, the bound cells were stained using fluorescent probes to evaluate their viability on the non-transparent gold surface. Furthermore, this piezoelectric biosensor was used for monitoring of drug-induced apoptosis initiated by micromolar concentrations of alpha-tocopherol amidomalate (alpha-TAM), a potential anticancer drug. The characteristic features of apoptosis as shrinkage and disintegration of cell-cell contacts could be monitored by QCM. The decrease of frequency and increase of resistance characterized gradual death of cells that was confirmed by fluorescence microscopy. The results indicate that the piezoelectric biosensor can be used for the real-time study of the cell adhesion and the screening of biologically active drugs affecting cellular behaviour. (C) 2012 Elsevier B.V. All rights reserved.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

FOHLEROVÁ, Zdenka, Jaroslav TURÁNEK a Petr SKLÁDAL. The cell adhesion and cytotoxicity effects of the derivate of vitamin E compared for two cell lines using a piezoelectric biosensor. Sensors and Actuators B Chemical. Amsterdam (Holandsko): Elsevier, 2012, roč. 174, November, s. 153-157. ISSN 0925-4005. Dostupné z: https://dx.doi.org/10.1016/j.snb.2012.08.047.

@article{1299732,
   author = {Fohlerová, Zdenka and Turánek, Jaroslav and Skládal, Petr},
   article_location = {Amsterdam (Holandsko)},
   article_number = {November},
   doi = {http://dx.doi.org/10.1016/j.snb.2012.08.047},
   keywords = {Quartz crystal microbalance; Fluorescence microscopy; Cell adhesion; Extracellular matrix; Apoptosis; Vitamin E},
   language = {eng},
   issn = {0925-4005},
   journal = {Sensors and Actuators B Chemical},
   title = {The cell adhesion and cytotoxicity effects of the derivate of vitamin E compared for two cell lines using a piezoelectric biosensor},
   url = {http://ac.els-cdn.com/S0925400512008611/1-s2.0-S0925400512008611-main.pdf?_tid=aecf51c4-ff94-11e4-83b5-00000aab0f6b&acdnat=1432197658_6fb3ce713a0c252bffd0a36ed1c9ba50},
   volume = {174},
   year = {2012}
}

TY  - JOUR
ID  - 1299732
AU  - Fohlerová, Zdenka - Turánek, Jaroslav - Skládal, Petr
PY  - 2012
TI  - The cell adhesion and cytotoxicity effects of the derivate of vitamin E compared for two cell lines using a piezoelectric biosensor
JF  - Sensors and Actuators B Chemical
VL  - 174
IS  - November
SP  - 153-157
EP  - 153-157
PB  - Elsevier
SN  - 09254005
KW  - Quartz crystal microbalance
KW  - Fluorescence microscopy
KW  - Cell adhesion
KW  - Extracellular matrix
KW  - Apoptosis
KW  - Vitamin E
UR  - http://ac.els-cdn.com/S0925400512008611/1-s2.0-S0925400512008611-main.pdf?_tid=aecf51c4-ff94-11e4-83b5-00000aab0f6b&acdnat=1432197658_6fb3ce713a0c252bffd0a36ed1c9ba50
L2  - http://ac.els-cdn.com/S0925400512008611/1-s2.0-S0925400512008611-main.pdf?_tid=aecf51c4-ff94-11e4-83b5-00000aab0f6b&acdnat=1432197658_6fb3ce713a0c252bffd0a36ed1c9ba50
N2  - The quartz crystal microbalance (QCM) was used to construct a piezoelectric biosensor utilizing rat liver epithelial cells (WB F344) and lung melanoma cells (B16F10) as biorecognition elements. The cells adhered on the gold surface modified by either hydrophobic polystyrene or fibronectin. The simultaneous monitoring of changes in the resonant frequency Delta f and the resistance Delta R corresponded with the mass and viscoelastic properties of the adhered cells. In addition, the bound cells were stained using fluorescent probes to evaluate their viability on the non-transparent gold surface. Furthermore, this piezoelectric biosensor was used for monitoring of drug-induced apoptosis initiated by micromolar concentrations of alpha-tocopherol amidomalate (alpha-TAM), a potential anticancer drug. The characteristic features of apoptosis as shrinkage and disintegration of cell-cell contacts could be monitored by QCM. The decrease of frequency and increase of resistance characterized gradual death of cells that was confirmed by fluorescence microscopy. The results indicate that the piezoelectric biosensor can be used for the real-time study of the cell adhesion and the screening of biologically active drugs affecting cellular behaviour. (C) 2012 Elsevier B.V. All rights reserved.
ER  -

FOHLEROVÁ, Zdenka, Jaroslav TURÁNEK a Petr SKLÁDAL. The cell adhesion and cytotoxicity effects of the derivate of vitamin E compared for two cell lines using a piezoelectric biosensor. \textit{Sensors and Actuators B Chemical}. Amsterdam (Holandsko): Elsevier, 2012, roč.~174, November, s.~153-157. ISSN~0925-4005. Dostupné z: https://dx.doi.org/10.1016/j.snb.2012.08.047.

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