Sub-Volume Averaging of Repetitive Structural Features in
Angularly Filtered Electron Tomographic Reconstructions

J 2014

Sub-Volume Averaging of Repetitive Structural Features in Angularly Filtered Electron Tomographic Reconstructions

KOVACIK, Lubomír; Sami KEREICHE; Pavel MATULA a Ivan RAŠKA

Základní údaje

Originální název

Sub-Volume Averaging of Repetitive Structural Features in Angularly Filtered Electron Tomographic Reconstructions

Autoři

KOVACIK, Lubomír (203 Česká republika); Sami KEREICHE (250 Francie); Pavel MATULA (203 Česká republika, domácí) a Ivan RAŠKA (203 Česká republika)

Vydání

Folia biologica, Prague, Charles University, 2014, 0015-5500

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.000

Kód RIV

RIV/00216224:14330/14:00080247

Organizační jednotka

Fakulta informatiky

UT WoS

000343275800011

Klíčová slova anglicky

electron tomography; missing wedge; limited specimen tilting; sub-volume averaging

Změněno: 22. 5. 2015 06:00, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

Electron tomographic reconstructions suffer from a number of artefacts arising from effects accompanying the processes of acquisition of a set of tilted projections of the specimen in a transmission electron microscope and from its subsequent computational handling. The most pronounced artefacts usually come from imprecise projection alignment, distortion of specimens during tomogram acquisition and from the presence of a region of missing data in the Fourier space, the "missing wedge". The ray artefacts caused by the presence of the missing wedge can be attenuated by the angular image filter, which attenuates the transition between the data and the missing wedge regions. In this work, we present an analysis of the influence of angular filtering on the resolution of averaged repetitive structural motives extracted from three-dimensional reconstructions of tomograms acquired in the single-axis tilting geometry.

Citovat

KOVACIK, Lubomír; Sami KEREICHE; Pavel MATULA a Ivan RAŠKA. Sub-Volume Averaging of Repetitive Structural Features in Angularly Filtered Electron Tomographic Reconstructions. Folia biologica. Prague: Charles University, 2014, roč. 60, Suppl. 1, s. 66-70. ISSN 0015-5500.

@article{1299814,
   author = {Kovacik, Lubomír and Kereiche, Sami and Matula, Pavel and Raška, Ivan},
   article_location = {Prague},
   article_number = {Suppl. 1},
   keywords = {electron tomography; missing wedge; limited specimen tilting; sub-volume averaging},
   language = {eng},
   issn = {0015-5500},
   journal = {Folia biologica},
   title = {Sub-Volume Averaging of Repetitive Structural Features in Angularly Filtered Electron Tomographic Reconstructions},
   volume = {60},
   year = {2014}
}

TY  - JOUR
ID  - 1299814
AU  - Kovacik, Lubomír - Kereiche, Sami - Matula, Pavel - Raška, Ivan
PY  - 2014
TI  - Sub-Volume Averaging of Repetitive Structural Features in Angularly Filtered Electron Tomographic Reconstructions
JF  - Folia biologica
VL  - 60
IS  - Suppl. 1
SP  - 66-70
EP  - 66-70
PB  - Charles University
SN  - 00155500
KW  - electron tomography
KW  - missing wedge
KW  - limited specimen tilting
KW  - sub-volume averaging
N2  - Electron tomographic reconstructions suffer from a number of artefacts arising from effects accompanying the processes of acquisition of a set of tilted projections of the specimen in a transmission electron microscope and from its subsequent computational handling. The most pronounced artefacts usually come from imprecise projection alignment, distortion of specimens during tomogram acquisition and from the presence of a region of missing data in the Fourier space, the "missing wedge". The ray artefacts caused by the presence of the missing wedge can be attenuated by the angular image filter, which attenuates the transition between the data and the missing wedge regions. In this work, we present an analysis of the influence of angular filtering on the resolution of averaged repetitive structural motives extracted from three-dimensional reconstructions of tomograms acquired in the single-axis tilting geometry.
ER  -

KOVACIK, Lubomír; Sami KEREICHE; Pavel MATULA a Ivan RAŠKA. Sub-Volume Averaging of Repetitive Structural Features in Angularly Filtered Electron Tomographic Reconstructions. \textit{Folia biologica}. Prague: Charles University, 2014, roč.~60, Suppl. 1, s.~66-70. ISSN~0015-5500.

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