Quartz crystal microbalance biosensor for rapid detection of
aerosolized microorganisms

D 2015

Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms

FARKA, Zdeněk, David KOVÁŘ a Petr SKLÁDAL

Základní údaje

Originální název

Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms

Autoři

FARKA, Zdeněk (203 Česká republika, domácí), David KOVÁŘ (203 Česká republika, domácí) a Petr SKLÁDAL (203 Česká republika, garant, domácí)

Vydání

USA, Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI, od s. 1-9, 9 s. 2015

Nakladatel

SPIE

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Kód RIV

RIV/00216224:14740/15:00082954

Organizační jednotka

Středoevropský technologický institut

ISBN

978-1-62841-571-1

ISSN

DOI

http://dx.doi.org/10.1117/12.2084606

UT WoS

000357744700006

Klíčová slova anglicky

Biosensor; Cyclone sampling; Quartz crystal microbalance; Bioaerosol; Escherichia coli

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 8. 2015 08:59, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Biological warfare agents (BWAs) represent the current menace of the asymmetric war. The early detection of BWAs, especially in the form of bioaerosol, is a challenging task for governments all around the world. Label-free quartz crystal microbalance (QCM) immunosensor and electrochemical immunosensor were developed and tested for rapid detection of BWA surrogate (E. coli) in the form of bioaerosol. Two immobilization strategies for the attachment of antibody were tested; the gold sensor surface was activated by cysteamine and then antibody was covalently linked either using glutaraldehyde, or the reduced antibodies were attached via Sulfo-SMCC. A portable bioaerosol chamber was constructed and used for safe manipulation with aerosolized microorganisms. The dissemination was done using a piezoelectric humidifier, distribution of bioaerosol inside the chamber was ensured using three 12-cm fans. The whole system was controlled remotely using LAN network. The disseminated microbial cells were collected and preconcentrated using the wetted-wall cyclone SASS 2300, the analysis was done using the on-line linked immunosensors. The QCM immunosensor had limit of detection 1×10^4 CFU·L-1 of air with analysis time 16 min, the whole experiment including dissemination and sensor surface regeneration took 40 min. In case of blank (disseminated sterile buffer), no signal change was observed. The electrochemical immunosensor was able to detect 150 CFU·L-1 of air in 20 min; also in this case, no interferences were observed. Reference measurements were done using particle counter Met One 3400 and by cultivation method on agar plates. The sensors have proved to be applicable for rapid screening of microorganisms in air.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

FARKA, Zdeněk, David KOVÁŘ a Petr SKLÁDAL. Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms. Online. In Augustus Way Fountain. Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI. USA: SPIE, 2015, s. 1-9. ISBN 978-1-62841-571-1. Dostupné z: https://dx.doi.org/10.1117/12.2084606.

@inproceedings{1299970,
   author = {Farka, Zdeněk and Kovář, David and Skládal, Petr},
   address = {USA},
   booktitle = {Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI},
   doi = {http://dx.doi.org/10.1117/12.2084606},
   editor = {Augustus Way Fountain},
   keywords = {Biosensor; Cyclone sampling; Quartz crystal microbalance; Bioaerosol; Escherichia coli},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {USA},
   isbn = {978-1-62841-571-1},
   pages = {1-9},
   publisher = {SPIE},
   title = {Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms},
   url = {http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2300142},
   year = {2015}
}

TY  - JOUR
ID  - 1299970
AU  - Farka, Zdeněk - Kovář, David - Skládal, Petr
PY  - 2015
TI  - Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms
PB  - SPIE
CY  - USA
SN  - 9781628415711
KW  - Biosensor
KW  - Cyclone sampling
KW  - Quartz crystal microbalance
KW  - Bioaerosol
KW  - Escherichia coli
UR  - http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2300142
N2  - Biological warfare agents (BWAs) represent the current menace of the asymmetric war. The early detection of BWAs, especially in the form of bioaerosol, is a challenging task for governments all around the world. Label-free quartz crystal microbalance (QCM) immunosensor and electrochemical immunosensor were developed and tested for rapid detection of BWA surrogate (E. coli) in the form of bioaerosol. Two immobilization strategies for the attachment of antibody were tested; the gold sensor surface was activated by cysteamine and then antibody was covalently linked either using glutaraldehyde, or the reduced antibodies were attached via Sulfo-SMCC. A portable bioaerosol chamber was constructed and used for safe manipulation with aerosolized microorganisms. The dissemination was done using a piezoelectric humidifier, distribution of bioaerosol inside the chamber was ensured using three 12-cm fans. The whole system was controlled remotely using LAN network. The disseminated microbial cells were collected and preconcentrated using the wetted-wall cyclone SASS 2300, the analysis was done using the on-line linked immunosensors. The QCM immunosensor had limit of detection 1×10^4 CFU·L-1 of air with analysis time 16 min, the whole experiment including dissemination and sensor surface regeneration took 40 min. In case of blank (disseminated sterile buffer), no signal change was observed. The electrochemical immunosensor was able to detect 150 CFU·L-1 of air in 20 min; also in this case, no interferences were observed. Reference measurements were done using particle counter Met One 3400 and by cultivation method on agar plates. The sensors have proved to be applicable for rapid screening of microorganisms in air.
ER  -

FARKA, Zdeněk, David KOVÁŘ a Petr SKLÁDAL. Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms. Online. In Augustus Way Fountain. \textit{Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI}. USA: SPIE, 2015, s.~1-9. ISBN~978-1-62841-571-1. Dostupné z: https://dx.doi.org/10.1117/12.2084606.

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