2015
Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms
FARKA, Zdeněk, David KOVÁŘ a Petr SKLÁDALZákladní údaje
Originální název
Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms
Autoři
FARKA, Zdeněk (203 Česká republika, domácí), David KOVÁŘ (203 Česká republika, domácí) a Petr SKLÁDAL (203 Česká republika, garant, domácí)
Vydání
USA, Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI, od s. 1-9, 9 s. 2015
Nakladatel
SPIE
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Forma vydání
elektronická verze "online"
Odkazy
Kód RIV
RIV/00216224:14740/15:00082954
Organizační jednotka
Středoevropský technologický institut
ISBN
978-1-62841-571-1
ISSN
UT WoS
000357744700006
Klíčová slova anglicky
Biosensor; Cyclone sampling; Quartz crystal microbalance; Bioaerosol; Escherichia coli
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 11. 8. 2015 08:59, doc. Mgr. Zdeněk Farka, Ph.D.
Anotace
V originále
Biological warfare agents (BWAs) represent the current menace of the asymmetric war. The early detection of BWAs, especially in the form of bioaerosol, is a challenging task for governments all around the world. Label-free quartz crystal microbalance (QCM) immunosensor and electrochemical immunosensor were developed and tested for rapid detection of BWA surrogate (E. coli) in the form of bioaerosol. Two immobilization strategies for the attachment of antibody were tested; the gold sensor surface was activated by cysteamine and then antibody was covalently linked either using glutaraldehyde, or the reduced antibodies were attached via Sulfo-SMCC. A portable bioaerosol chamber was constructed and used for safe manipulation with aerosolized microorganisms. The dissemination was done using a piezoelectric humidifier, distribution of bioaerosol inside the chamber was ensured using three 12-cm fans. The whole system was controlled remotely using LAN network. The disseminated microbial cells were collected and preconcentrated using the wetted-wall cyclone SASS 2300, the analysis was done using the on-line linked immunosensors. The QCM immunosensor had limit of detection 1×10^4 CFU·L-1 of air with analysis time 16 min, the whole experiment including dissemination and sensor surface regeneration took 40 min. In case of blank (disseminated sterile buffer), no signal change was observed. The electrochemical immunosensor was able to detect 150 CFU·L-1 of air in 20 min; also in this case, no interferences were observed. Reference measurements were done using particle counter Met One 3400 and by cultivation method on agar plates. The sensors have proved to be applicable for rapid screening of microorganisms in air.
Návaznosti
ED1.1.00/02.0068, projekt VaV |
|