Characterization of the Role of Collagen Network Structure and
Composition in Cancer Cell Migration

D 2015

Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration

ANGUIANO, María, Carlos CASTILLA, Martin MAŠKA, Cristina EDERRA, Javier FERNÁNDEZ-MARQUÉS et. al.

Základní údaje

Originální název

Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration

Autoři

ANGUIANO, María (724 Španělsko), Carlos CASTILLA (724 Španělsko), Martin MAŠKA (203 Česká republika, garant, domácí), Cristina EDERRA (724 Španělsko), Javier FERNÁNDEZ-MARQUÉS (724 Španělsko), Rafael PELÁEZ (724 Španělsko), Ana ROUZAUT (724 Španělsko), Arrate MUÑOZ-BARRUTIA (724 Španělsko), Michal KOZUBEK (203 Česká republika, domácí) a Carlos ORTIZ-DE-SOLÓRZANO (724 Španělsko)

Vydání

Milano, 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, od s. 8139-8142, 4 s. 2015

Nakladatel

IEEE

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10201 Computer sciences, information science, bioinformatics

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Kód RIV

RIV/00216224:14330/15:00080823

Organizační jednotka

Fakulta informatiky

ISBN

978-1-4244-9271-8

ISSN

DOI

http://dx.doi.org/10.1109/EMBC.2015.7320283

UT WoS

000371717208103

Klíčová slova anglicky

collagen network geometry; confocal reflection microscopy; Matrigel; steerable filtering; pore size; cell migration

Štítky

cbia-web, firank_B

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 4. 2016 14:15, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

The geometry of 3D collagen networks is a key factor that influences the behavior of live cells within extracellular matrices. This paper presents a method for automatic quantification of the 3D collagen network geometry with fiber resolution in confocal reflection microscopy images. The proposed method is based on a smoothing filter and binarization of the collagen network followed by a fiber reconstruction algorithm. The method is validated on 3D collagen gels with various collagen and Matrigel concentrations. The results reveal that Matrigel affects the collagen network geometry by decreasing the network pore size while preserving the fiber length and fiber persistence length. The influence of network composition and geometry, especially pore size, is preliminarily analyzed by quantifying the migration patterns of lung cancer cells within microfluidic devices filled with three different hydrogel types. The experiments reveal that Matrigel, while decreasing pore size, stimulates cell migration. Further studies on this relationship could be instrumental for the study of cancer metastasis and other biological processes involving cell migration.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

ANGUIANO, María, Carlos CASTILLA, Martin MAŠKA, Cristina EDERRA, Javier FERNÁNDEZ-MARQUÉS, Rafael PELÁEZ, Ana ROUZAUT, Arrate MUÑOZ-BARRUTIA, Michal KOZUBEK a Carlos ORTIZ-DE-SOLÓRZANO. Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration. Online. In 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Milano: IEEE, 2015, s. 8139-8142. ISBN 978-1-4244-9271-8. Dostupné z: https://dx.doi.org/10.1109/EMBC.2015.7320283.

@inproceedings{1300413,
   author = {Anguiano, María and Castilla, Carlos and Maška, Martin and Ederra, Cristina and FernándezandMarqués, Javier and Peláez, Rafael and Rouzaut, Ana and MuñozandBarrutia, Arrate and Kozubek, Michal and OrtizanddeandSolórzano, Carlos},
   address = {Milano},
   booktitle = {37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society},
   doi = {http://dx.doi.org/10.1109/EMBC.2015.7320283},
   keywords = {collagen network geometry; confocal reflection microscopy; Matrigel; steerable filtering; pore size; cell migration},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Milano},
   isbn = {978-1-4244-9271-8},
   pages = {8139-8142},
   publisher = {IEEE},
   title = {Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration},
   url = {http://dx.doi.org/10.1109/EMBC.2015.7320283},
   year = {2015}
}

TY  - JOUR
ID  - 1300413
AU  - Anguiano, María - Castilla, Carlos - Maška, Martin - Ederra, Cristina - Fernández-Marqués, Javier - Peláez, Rafael - Rouzaut, Ana - Muñoz-Barrutia, Arrate - Kozubek, Michal - Ortiz-de-Solórzano, Carlos
PY  - 2015
TI  - Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration
PB  - IEEE
CY  - Milano
SN  - 9781424492718
KW  - collagen network geometry
KW  - confocal reflection microscopy
KW  - Matrigel
KW  - steerable filtering
KW  - pore size
KW  - cell migration
UR  - http://dx.doi.org/10.1109/EMBC.2015.7320283
N2  - The geometry of 3D collagen networks is a key factor that influences the behavior of live cells within extracellular matrices. This paper presents a method for automatic quantification of the 3D collagen network geometry with fiber resolution in confocal reflection microscopy images. The proposed method is based on a smoothing filter and binarization of the collagen network followed by a fiber reconstruction algorithm. The method is validated on 3D collagen gels with various collagen and Matrigel concentrations. The results reveal that Matrigel affects the collagen network geometry by decreasing the network pore size while preserving the fiber length and fiber persistence length. The influence of network composition and geometry, especially pore size, is preliminarily analyzed by quantifying the migration patterns of lung cancer cells within microfluidic devices filled with three different hydrogel types. The experiments reveal that Matrigel, while decreasing pore size, stimulates cell migration. Further studies on this relationship could be instrumental for the study of cancer metastasis and other biological processes involving cell migration.
ER  -

ANGUIANO, María, Carlos CASTILLA, Martin MAŠKA, Cristina EDERRA, Javier FERNÁNDEZ-MARQUÉS, Rafael PELÁEZ, Ana ROUZAUT, Arrate MUÑOZ-BARRUTIA, Michal KOZUBEK a Carlos ORTIZ-DE-SOLÓRZANO. Characterization of the Role of Collagen Network Structure and Composition in Cancer Cell Migration. Online. In \textit{37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society}. Milano: IEEE, 2015, s.~8139-8142. ISBN~978-1-4244-9271-8. Dostupné z: https://dx.doi.org/10.1109/EMBC.2015.7320283.

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