k 2015

Characterization of autotrophic growth of a model cyanobacterium Synechocystis sp. PCC 6803 in a flat panel photobioreactor

ZAVŘEL, Tomáš; Petra OČENÁŠOVÁ; Maria SINETOVA a Jan ČERVENÝ

Základní údaje

Originální název

Characterization of autotrophic growth of a model cyanobacterium Synechocystis sp. PCC 6803 in a flat panel photobioreactor

Název anglicky

Characterization of autotrophic growth of a model cyanobacterium Synechocystis sp. PCC 6803 in a flat panel photobioreactor

Autoři

ZAVŘEL, Tomáš; Petra OČENÁŠOVÁ; Maria SINETOVA a Jan ČERVENÝ

Vydání

Global Change: A Complex Challenge, 2015

Další údaje

Typ výsledku

Prezentace na konferencích

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ne
Změněno: 8. 6. 2015 22:25, Mgr. Tomáš Zavřel, Ph.D.

Anotace

ORIG EN

V originále

Cyanobacteria have gained increased attention as ideal candidates for biotechnological applications due to their capacity to produce valuable molecules ranging from therapeutic proteins to biofuels. Their natural phenotypic plasticity in highly dynamic environments allows easy deployment of new biotechnologies as well as open possibility for genetic engineering. Here, we present new approach to fast and reliable characterization of cyanobacteria growth in a flat panel photobioreactor examined for changing light, temperature, inorganic carbon and other nutrients availability. The utilization of semi-continuous automatic cultivation with real-time culture growth monitoring provides strong experimental base for both characterization and optimization of cyanobacteria cultures in photobioreactors. At first, we characterized autotrophic growth of Synechocystis sp. PCC 6803 strain denoted as GT-L1,2. This strain is capable of efficient growth under wide range of environmental conditions with doubling time as fast as 5 hours under favorable conditions. However, differences between Synechocystis sp. PCC 6803 substrains have been identified on both genome and phenotype levels3–5. Thus, we aim on utilization of our experimental platform for characterization of multiple commonly used Synechocystis substrains. This will allow us to identify strains capable of robust growth and high production yields as reliable biotechnological candidates.

Anglicky

Cyanobacteria have gained increased attention as ideal candidates for biotechnological applications due to their capacity to produce valuable molecules ranging from therapeutic proteins to biofuels. Their natural phenotypic plasticity in highly dynamic environments allows easy deployment of new biotechnologies as well as open possibility for genetic engineering. Here, we present new approach to fast and reliable characterization of cyanobacteria growth in a flat panel photobioreactor examined for changing light, temperature, inorganic carbon and other nutrients availability. The utilization of semi-continuous automatic cultivation with real-time culture growth monitoring provides strong experimental base for both characterization and optimization of cyanobacteria cultures in photobioreactors. At first, we characterized autotrophic growth of Synechocystis sp. PCC 6803 strain denoted as GT-L1,2. This strain is capable of efficient growth under wide range of environmental conditions with doubling time as fast as 5 hours under favorable conditions. However, differences between Synechocystis sp. PCC 6803 substrains have been identified on both genome and phenotype levels3–5. Thus, we aim on utilization of our experimental platform for characterization of multiple commonly used Synechocystis substrains. This will allow us to identify strains capable of robust growth and high production yields as reliable biotechnological candidates.