Moderate hypothermia during ex vivo machine perfusion promotes
recovery of hearts donated after cardiocirculatory death

J 2016

Moderate hypothermia during ex vivo machine perfusion promotes recovery of hearts donated after cardiocirculatory death

TOLBOOM, Herman; Veronika OLEJNÍČKOVÁ; Diana RESER; Barbara ROSSER; Markus J. WILHELM et al.

Základní údaje

Originální název

Moderate hypothermia during ex vivo machine perfusion promotes recovery of hearts donated after cardiocirculatory death

Autoři

TOLBOOM, Herman; Veronika OLEJNÍČKOVÁ; Diana RESER; Barbara ROSSER; Markus J. WILHELM; Max GASSMANN; Anna BOGDANOVA a Volkmar FALK

Vydání

European Journal Cardio-Thoracic Surgery, Cary, Oxford University Press, 2016, 1010-7940

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.759

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/16:00089079

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Heart transplantation; Organ preservation; Machine perfusion; Declaration of cardiocirculatory death; Ischaemia-reperfusion

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 8. 2016 10:59, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

OBJECTIVES To establish the optimal machine perfusion temperature for recovery of hearts in a rodent model of donation after declaration of cardiocirculatory death (DCD). METHODS Hearts from male Lewis rats (n = 14/group) were subjected to 25 min of in situ warm (37°C) ischaemia to simulate DCD. They were then explanted and reperfused with diluted autologous blood for 60 min at 20, 25, 30, 33 or 37°C, after which they were stored at 0-4°C in Custodiol preservation solution for 240 min. Fresh-excised and cold-stored ischaemic hearts were used as controls. The viability of the different groups was assessed by comparing heart rate and left ventricular contractility in a Langendorff circuit, as well as perfusate levels of troponin-t and creatine kinase (CK), and myocardial levels of adenosine triphosphate (ATP) and reduced glutathione. RESULTS During ex vivo reperfusion, hearts in all groups resumed beating within minutes. The mean heart rate was highest in the 37°C group at 154.72 +/- 33.01 beats × min-1 (bpm), and declined in proportion to temperature to 39.72 +/- 5.53 bpm at 20°C. Troponin-t levels were highest in the 37°C group (79.49 +/- 20.79 µg/l), the values were significantly lower in all other reconditioned groups with a minimum of 12.472 +/- 7.08 µg/l in the 20°C group (P < 0.0001). Tissue ATP levels ranged from 4.32 ± 1.71 µmol/g at 33°C to 4.59 +/- 1.41 µmol/g at 30°C, all significantly higher than the mean ATP level of 1.41 +/- 0.93 µmol/g in untreated ischaemic hearts (P 0.0001). During Langendorff assessment, the mean heart rate and contractility of all groups were higher than those of cold-stored ischaemic hearts (P 0.0001), yet not significantly different from those of fresh controls. The perfusate levels of troponin-t and CK, and myocardial levels of reduced-glutathione and ATP were not significantly different between groups. CONCLUSION Our results suggest that mild hypothermia during ex vivo reperfusion improves recovery of ischaemic hearts in a rodent DCD model.

Citovat

TOLBOOM, Herman; Veronika OLEJNÍČKOVÁ; Diana RESER; Barbara ROSSER; Markus J. WILHELM; Max GASSMANN; Anna BOGDANOVA a Volkmar FALK. Moderate hypothermia during ex vivo machine perfusion promotes recovery of hearts donated after cardiocirculatory death. European Journal Cardio-Thoracic Surgery. Cary: Oxford University Press, 2016, roč. 49, č. 1, s. 25-31. ISSN 1010-7940. Dostupné z: https://doi.org/10.1093/ejcts/ezv066.

@article{1301211,
   author = {Tolboom, Herman and Olejníčková, Veronika and Reser, Diana and Rosser, Barbara and Wilhelm, Markus J. and Gassmann, Max and Bogdanova, Anna and Falk, Volkmar},
   article_location = {Cary},
   article_number = {1},
   doi = {https://doi.org/10.1093/ejcts/ezv066},
   keywords = {Heart transplantation; Organ preservation; Machine perfusion; Declaration of cardiocirculatory death; Ischaemia-reperfusion},
   language = {eng},
   issn = {1010-7940},
   journal = {European Journal Cardio-Thoracic Surgery},
   title = {Moderate hypothermia during ex vivo machine perfusion promotes recovery of hearts donated after cardiocirculatory death},
   volume = {49},
   year = {2016}
}

TY  - JOUR
ID  - 1301211
AU  - Tolboom, Herman - Olejníčková, Veronika - Reser, Diana - Rosser, Barbara - Wilhelm, Markus J. - Gassmann, Max - Bogdanova, Anna - Falk, Volkmar
PY  - 2016
TI  - Moderate hypothermia during ex vivo machine perfusion promotes recovery of hearts donated after cardiocirculatory death
JF  - European Journal Cardio-Thoracic Surgery
VL  - 49
IS  - 1
SP  - 25-31
EP  - 25-31
PB  - Oxford University Press
SN  - 10107940
KW  - Heart transplantation
KW  - Organ preservation
KW  - Machine perfusion
KW  - Declaration of cardiocirculatory death
KW  - Ischaemia-reperfusion
N2  - OBJECTIVES To establish the optimal machine perfusion temperature for recovery of hearts in a rodent model of donation after declaration of cardiocirculatory death (DCD). METHODS Hearts from male Lewis rats (n = 14/group) were subjected to 25 min of in situ warm (37°C) ischaemia to simulate DCD. They were then explanted and reperfused with diluted autologous blood for 60 min at 20, 25, 30, 33 or 37°C, after which they were stored at 0-4°C in Custodiol preservation solution for 240 min. Fresh-excised and cold-stored ischaemic hearts were used as controls. The viability of the different groups was assessed by comparing heart rate and left ventricular contractility in a Langendorff circuit, as well as perfusate levels of troponin-t and creatine kinase (CK), and myocardial levels of adenosine triphosphate (ATP) and reduced glutathione. RESULTS During ex vivo reperfusion, hearts in all groups resumed beating within minutes. The mean heart rate was highest in the 37°C group at 154.72 +/- 33.01 beats × min-1 (bpm), and declined in proportion to temperature to 39.72 +/- 5.53 bpm at 20°C. Troponin-t levels were highest in the 37°C group (79.49 +/- 20.79 µg/l), the values were significantly lower in all other reconditioned groups with a minimum of 12.472 +/- 7.08 µg/l in the 20°C group (P < 0.0001). Tissue ATP levels ranged from 4.32 ± 1.71 µmol/g at 33°C to 4.59 +/- 1.41 µmol/g at 30°C, all significantly higher than the mean ATP level of 1.41 +/- 0.93 µmol/g in untreated ischaemic hearts (P 0.0001). During Langendorff assessment, the mean heart rate and contractility of all groups were higher than those of cold-stored ischaemic hearts (P 0.0001), yet not significantly different from those of fresh controls. The perfusate levels of troponin-t and CK, and myocardial levels of reduced-glutathione and ATP were not significantly different between groups. CONCLUSION Our results suggest that mild hypothermia during ex vivo reperfusion improves recovery of ischaemic hearts in a rodent DCD model.
ER  -

TOLBOOM, Herman; Veronika OLEJNÍČKOVÁ; Diana RESER; Barbara ROSSER; Markus J. WILHELM; Max GASSMANN; Anna BOGDANOVA a Volkmar FALK. Moderate hypothermia during ex vivo machine perfusion promotes recovery of hearts donated after cardiocirculatory death. \textit{European Journal Cardio-Thoracic Surgery}. Cary: Oxford University Press, 2016, roč.~49, č.~1, s.~25-31. ISSN~1010-7940. Dostupné z: https://doi.org/10.1093/ejcts/ezv066.

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