Tumor suppressor candidate 3 (TUSC3) prevents the epithelialto-
mesenchymal transition and inhibits tumor growth by modulating
the endoplasmic reticulum stress response in ovarian cancer
cells

J 2015

Tumor suppressor candidate 3 (TUSC3) prevents the epithelialto- mesenchymal transition and inhibits tumor growth by modulating the endoplasmic reticulum stress response in ovarian cancer cells

KRATOCHVÍLOVÁ, Kateřina; Peter HORAK; Milan EŠNER; Karel SOUČEK; Dietmar PILS et al.

Základní údaje

Originální název

Tumor suppressor candidate 3 (TUSC3) prevents the epithelialto- mesenchymal transition and inhibits tumor growth by modulating the endoplasmic reticulum stress response in ovarian cancer cells

Autoři

Vydání

International Journal of Cancer, Hoboken, Wiley-Blackwell, 2015, 0020-7136

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.531

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/15:00083329

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

TUSC3; N33; endoplasmic reticulum stress; epithelial-to-mesenchymal transition; ovarian cancer; tumor suppressor

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 11. 2015 14:48, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Ovarian cancer is one of the most common malignancies in women and contributes greatly to cancer-related deaths. Tumor suppressor candidate 3 (TUSC3) is a putative tumor suppressor gene located at chromosomal region 8p22, which is often lost in epithelial cancers. Epigenetic silencing of TUSC3 has been associated with poor prognosis, and hypermethylation of its promoter provides an independent biomarker of overall and disease-free survival in ovarian cancer patients. TUSC3 is localized to the endoplasmic reticulum in an oligosaccharyl tranferase complex responsible for the N-glycosylation of proteins. However, the precise molecular role of TUSC3 in ovarian cancer remains unclear. In this study, we establish TUSC3 as a novel ovarian cancer tumor suppressor using a xenograft mouse model and demonstrate that loss of TUSC3 alters the molecular response to endoplasmic reticulum stress and induces hallmarks of the epithelial-to-mesenchymal transition in ovarian cancer cells. In summary, we have confirmed the tumor-suppressive function of TUSC3 and identified the possible mechanism driving TUSC3-deficient ovarian cancer cells toward a malignant phenotype.

Návaznosti

EE2.3.20.0185, projekt VaV

Název: Centrum analýz a modelování tkání a orgánů

MUNI/A/1558/2014, interní kód MU

Název: Zdroje pro tkáňové inženýrství 5 (Akronym: TissueEng5)

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 5, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

MUNI/M/1050/2013, interní kód MU

Název: Automatizovaná analýza elektronmikroskopických snímků pro použití v biologii a medicíně (Akronym: Analýza TEM snímků)

Investor: Masarykova univerzita, Automatizovaná analýza elektronmikroskopických snímků pro použití v biologii a medicíně, INTERDISCIPLINARY - Mezioborové výzkumné projekty

Citovat

KRATOCHVÍLOVÁ, Kateřina; Peter HORAK; Milan EŠNER; Karel SOUČEK; Dietmar PILS; Mariam ANEES; Erwin TOMASICH; František DRÁFI; Veronika JURTÍKOVÁ; Aleš HAMPL; Michael KRAINER a Petr VAŇHARA. Tumor suppressor candidate 3 (TUSC3) prevents the epithelialto- mesenchymal transition and inhibits tumor growth by modulating the endoplasmic reticulum stress response in ovarian cancer cells. International Journal of Cancer. Hoboken: Wiley-Blackwell, 2015, roč. 137, č. 6, s. 1330-1340. ISSN 0020-7136. Dostupné z: https://doi.org/10.1002/ijc.29502.

@article{1305771,
   author = {Kratochvílová, Kateřina and Horak, Peter and Ešner, Milan and Souček, Karel and Pils, Dietmar and Anees, Mariam and Tomasich, Erwin and Dráfi, František and Jurtíková, Veronika and Hampl, Aleš and Krainer, Michael and Vaňhara, Petr},
   article_location = {Hoboken},
   article_number = {6},
   doi = {https://doi.org/10.1002/ijc.29502},
   keywords = {TUSC3; N33; endoplasmic reticulum stress; epithelial-to-mesenchymal transition; ovarian cancer; tumor suppressor},
   language = {eng},
   issn = {0020-7136},
   journal = {International Journal of Cancer},
   title = {Tumor suppressor candidate 3 (TUSC3) prevents the epithelialto- mesenchymal transition and inhibits tumor growth by modulating the endoplasmic reticulum stress response in ovarian cancer cells},
   volume = {137},
   year = {2015}
}

TY  - JOUR
ID  - 1305771
AU  - Kratochvílová, Kateřina - Horak, Peter - Ešner, Milan - Souček, Karel - Pils, Dietmar - Anees, Mariam - Tomasich, Erwin - Dráfi, František - Jurtíková, Veronika - Hampl, Aleš - Krainer, Michael - Vaňhara, Petr
PY  - 2015
TI  - Tumor suppressor candidate 3 (TUSC3) prevents the epithelialto- mesenchymal transition and inhibits tumor growth by modulating the endoplasmic reticulum stress response in ovarian cancer cells
JF  - International Journal of Cancer
VL  - 137
IS  - 6
SP  - 1330-1340
EP  - 1330-1340
PB  - Wiley-Blackwell
SN  - 00207136
KW  - TUSC3
KW  - N33
KW  - endoplasmic reticulum stress
KW  - epithelial-to-mesenchymal transition
KW  - ovarian cancer
KW  - tumor suppressor
N2  - Ovarian cancer is one of the most common malignancies in women and contributes greatly to cancer-related deaths. Tumor suppressor candidate 3 (TUSC3) is a putative tumor suppressor gene located at chromosomal region 8p22, which is often lost in epithelial cancers. Epigenetic silencing of TUSC3 has been associated with poor prognosis, and hypermethylation of its promoter provides an independent biomarker of overall and disease-free survival in ovarian cancer patients. TUSC3 is localized to the endoplasmic reticulum in an oligosaccharyl tranferase complex responsible for the N-glycosylation of proteins. However, the precise molecular role of TUSC3 in ovarian cancer remains unclear. In this study, we establish TUSC3 as a novel ovarian cancer tumor suppressor using a xenograft mouse model and demonstrate that loss of TUSC3 alters the molecular response to endoplasmic reticulum stress and induces hallmarks of the epithelial-to-mesenchymal transition in ovarian cancer cells. In summary, we have confirmed the tumor-suppressive function of TUSC3 and identified the possible mechanism driving TUSC3-deficient ovarian cancer cells toward a malignant phenotype.
ER  -

KRATOCHVÍLOVÁ, Kateřina; Peter HORAK; Milan EŠNER; Karel SOUČEK; Dietmar PILS; Mariam ANEES; Erwin TOMASICH; František DRÁFI; Veronika JURTÍKOVÁ; Aleš HAMPL; Michael KRAINER a Petr VAŇHARA. Tumor suppressor candidate 3 (TUSC3) prevents the epithelialto- mesenchymal transition and inhibits tumor growth by modulating the endoplasmic reticulum stress response in ovarian cancer cells. \textit{International Journal of Cancer}. Hoboken: Wiley-Blackwell, 2015, roč.~137, č.~6, s.~1330-1340. ISSN~0020-7136. Dostupné z: https://doi.org/10.1002/ijc.29502.

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