Sensitive determination of glutathione in biological samples by
capillary electrophoresis with green (515 nm) laser-induced
fluorescence detection

J 2015

Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection

HODÁKOVÁ, Júlia; Jan PREISLER; František FORET a Petr KUBÁŇ

Základní údaje

Originální název

Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection

Autoři

HODÁKOVÁ, Júlia; Jan PREISLER; František FORET a Petr KUBÁŇ

Vydání

Journal of Chromatography A, AMSTERDAM, Elsevier, 2015, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.926

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/15:00080903

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Capillary electrophoresis; Laser-induced fluorescence; Glutathione; Eosin-5-maleimide

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 2. 2018 22:06, prof. Mgr. Jan Preisler, Ph.D.

Anotace

V originále

A new sensitive capillary electrophoretic method with laser-induced fluorescence (LIF) was developed for quantitation of glutathione (GSH) in biological samples. Eosin-5-maleimide was used to label the GSH molecule and the formed conjugate was separated in a 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid electrolyte at pH 7.0 in less than 3 min. The conjugate was detected with an in-house built LIF system, utilizing an inexpensive 515 nm diode laser module. Studies were performed to optimize the derivatizatiory(the ratio of reagent to analyte, the reaction time, pH, etc.) and separation conditions. Sensitive detection of GSH at concentrations as low as 0.18 nM was obtained. The method was applied in the analysis of biological fluids (exhaled breath condensate, saliva) and was found to be suitable for determination of GSH in these samples at trace levels below 1 nM. To the best of our knowledge, this is the first report on determination of GSH in exhaled breath condensate by capillary electrophoresis (CE). (C) 2015 Elsevier B.V. All rights reserved.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GA13-21919S, projekt VaV

Název: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.

Investor: Grantová agentura ČR, Vývoj neinvazivních metod odberu vzorku a analýzy v point-of-care dignostice biologických materiálu

Citovat

HODÁKOVÁ, Júlia; Jan PREISLER; František FORET a Petr KUBÁŇ. Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection. Journal of Chromatography A. AMSTERDAM: Elsevier, 2015, roč. 1391, April, s. 102-108. ISSN 0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2015.02.062.

@article{1306292,
   author = {Hodáková, Júlia and Preisler, Jan and Foret, František and Kubáň, Petr},
   article_location = {AMSTERDAM},
   article_number = {April},
   doi = {https://doi.org/10.1016/j.chroma.2015.02.062},
   keywords = {Capillary electrophoresis; Laser-induced fluorescence; Glutathione; Eosin-5-maleimide},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection},
   volume = {1391},
   year = {2015}
}

TY  - JOUR
ID  - 1306292
AU  - Hodáková, Júlia - Preisler, Jan - Foret, František - Kubáň, Petr
PY  - 2015
TI  - Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection
JF  - Journal of Chromatography A
VL  - 1391
IS  - April
SP  - 102-108
EP  - 102-108
PB  - Elsevier
SN  - 00219673
KW  - Capillary electrophoresis
KW  - Laser-induced fluorescence
KW  - Glutathione
KW  - Eosin-5-maleimide
N2  - A new sensitive capillary electrophoretic method with laser-induced fluorescence (LIF) was developed for quantitation of glutathione (GSH) in biological samples. Eosin-5-maleimide was used to label the GSH molecule and the formed conjugate was separated in a 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid electrolyte at pH 7.0 in less than 3 min. The conjugate was detected with an in-house built LIF system, utilizing an inexpensive 515 nm diode laser module. Studies were performed to optimize the derivatizatiory(the ratio of reagent to analyte, the reaction time, pH, etc.) and separation conditions. Sensitive detection of GSH at concentrations as low as 0.18 nM was obtained. The method was applied in the analysis of biological fluids (exhaled breath condensate, saliva) and was found to be suitable for determination of GSH in these samples at trace levels below 1 nM. To the best of our knowledge, this is the first report on determination of GSH in exhaled breath condensate by capillary electrophoresis (CE). (C) 2015 Elsevier B.V. All rights reserved.
ER  -

HODÁKOVÁ, Júlia; Jan PREISLER; František FORET a Petr KUBÁŇ. Sensitive determination of glutathione in biological samples by capillary electrophoresis with green (515 nm) laser-induced fluorescence detection. \textit{Journal of Chromatography A}. AMSTERDAM: Elsevier, 2015, roč.~1391, April, s.~102-108. ISSN~0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2015.02.062.

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