SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL
SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED
FLUORESCENCE DETECTION

D 2014

SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION

LAČNÁ, Júlia; Jan PREISLER; František FORET a Petr KUBÁŇ

Základní údaje

Originální název

SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION

Autoři

LAČNÁ, Júlia; Jan PREISLER; František FORET a Petr KUBÁŇ

Vydání

BRNO, CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS, od s. 232-236, 5 s. 2014

Nakladatel

INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/14:00083392

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-904959-2-0

UT WoS

000354547400077

Klíčová slova anglicky

GLUTATHIONE; fluorescence

Štítky

AKR, rivok

Změněno: 15. 2. 2018 21:41, prof. Mgr. Jan Preisler, Ph.D.

Anotace

V originále

Laser induced fluorescence capillary electrophoresis (CE-LIF) with 515 nm green laser module was used for sensitive determination of glutathione (GSH) in various biological samples. For the first time, eosin-5-maleimide was used as a fluorescent tag in CE. Studies were performed to optimize the derivatization conditions (the ratio of reagent to analyte, the reaction time, temperature, etc.). CE-LIF also offers a unique opportunity to monitor the stability of the reagent and various reaction products. We demonstrate the applicability of the developed method on determination of GSH in real biological samples (exhaled breath condensate and saliva) with LODs as low as 180 pM.

Citovat

LAČNÁ, Júlia; Jan PREISLER; František FORET a Petr KUBÁŇ. SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION. In Foret, F Krenkova, J Drobnikova, I Guttman, A Kleparnik, K. CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS. BRNO: INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC, 2014, s. 232-236. ISBN 978-80-904959-2-0.

@inproceedings{1306293,
   author = {Lačná, Júlia and Preisler, Jan and Foret, František and Kubáň, Petr},
   address = {BRNO},
   booktitle = {CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS},
   editor = {Foret, F Krenkova, J Drobnikova, I Guttman, A Kleparnik, K},
   keywords = {GLUTATHIONE; fluorescence},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {BRNO},
   isbn = {978-80-904959-2-0},
   pages = {232-236},
   publisher = {INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC},
   title = {SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION},
   year = {2014}
}

TY  - CONF
ID  - 1306293
AU  - Lačná, Júlia - Preisler, Jan - Foret, František - Kubáň, Petr
PY  - 2014
TI  - SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION
PB  - INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC
CY  - BRNO
SN  - 9788090495920
KW  - GLUTATHIONE
KW  - fluorescence
N2  - Laser induced fluorescence capillary electrophoresis (CE-LIF) with 515 nm green laser module was used for sensitive determination of glutathione (GSH) in various biological samples. For the first time, eosin-5-maleimide was used as a fluorescent tag in CE. Studies were performed to optimize the derivatization conditions (the ratio of reagent to analyte, the reaction time, temperature, etc.). CE-LIF also offers a unique opportunity to monitor the stability of the reagent and various reaction products. We demonstrate the applicability of the developed method on determination of GSH in real biological samples (exhaled breath condensate and saliva) with LODs as low as 180 pM.
ER  -

LAČNÁ, Júlia; Jan PREISLER; František FORET a Petr KUBÁŇ. SENSITIVE DETERMINATION OF GLUTATHIONE IN VARIOUS BIOLOGICAL SAMPLES BY CAPILLARY ELECTROPHORESIS WITH GREEN LASER INDUCED FLUORESCENCE DETECTION. In Foret, F Krenkova, J Drobnikova, I Guttman, A Kleparnik, K. \textit{CECE 2014: 11TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS}. BRNO: INST ANALYTICAL CHEMISTRY ASCR, V V I-IAC, 2014, s.~232-236. ISBN~978-80-904959-2-0.

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