Role of Hypoxia-Induced Brain Derived Neurotrophic Factor in
Human Pulmonary Artery Smooth Muscle

J 2015

Role of Hypoxia-Induced Brain Derived Neurotrophic Factor in Human Pulmonary Artery Smooth Muscle

HARTMAN, William; Martin HELÁN; Dan SMELTER; Venkatashelem SATHISH; Michael THOMPSON et al.

Základní údaje

Originální název

Role of Hypoxia-Induced Brain Derived Neurotrophic Factor in Human Pulmonary Artery Smooth Muscle

Autoři

HARTMAN, William; Martin HELÁN; Dan SMELTER; Venkatashelem SATHISH; Michael THOMPSON; Chistina M. PABELICK; Bruce JOHNSON a Y.S. PRAKASH

Vydání

PLOS ONE, San Francisco, Public Library of Science, 2015, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30201 Cardiac and Cardiovascular systems

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Odkaz na fulltext článku

Impakt faktor

Impact factor: 3.057

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/15:00083443

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

BDNF; Hypoxia; Pulmonary Artery

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 4. 2016 13:42, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Hypoxia effects on pulmonary artery structure and function are key to diseases such as pulmonary hypertension. Recent studies suggest that growth factors called neurotrophins, particularly brain-derived neurotrophic factor (BDNF), can influence lung structure and function, and their role in the pulmonary artery warrants further investigation. In this study, we examined the effect of hypoxia on BDNF in humans, and the influence of hypoxiaenhanced BDNF expression and signaling in human pulmonary artery smooth muscle cells (PASMCs). 48h of 1% hypoxia enhanced BDNF and TrkB expression, as well as release of BDNF. In arteries of patients with pulmonary hypertension, BDNF expression and release was higher at baseline. In isolated PASMCs, hypoxia-induced BDNF increased intracellular Ca2+ responses to serotonin: an effect altered by HIF1 inhibition or by neutralization of extracellular BDNF via chimeric TrkB-Fc. Enhanced BDNF/TrkB signaling increased PASMC survival and proliferation, and decreased apoptosis following hypoxia. Enhanced expression and signaling of the BDNF-TrkB system in PASMCs is a potential mechanism by which hypoxia can promote changes in pulmonary artery structure and function. Accordingly, the BDNF-TrkB system could be a key player in the pathogenesis of hypoxia- induced pulmonary vascular diseases, and thus a potential target for therapy.

Citovat

HARTMAN, William; Martin HELÁN; Dan SMELTER; Venkatashelem SATHISH; Michael THOMPSON; Chistina M. PABELICK; Bruce JOHNSON a Y.S. PRAKASH. Role of Hypoxia-Induced Brain Derived Neurotrophic Factor in Human Pulmonary Artery Smooth Muscle. PLOS ONE. San Francisco: Public Library of Science, 2015, roč. 10, č. 7, s. "e0129489", 17 s. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0129489.

@article{1306908,
   author = {Hartman, William and Helán, Martin and Smelter, Dan and Sathish, Venkatashelem and Thompson, Michael and Pabelick, Chistina M. and Johnson, Bruce and Prakash, Y.S.},
   article_location = {San Francisco},
   article_number = {7},
   doi = {https://doi.org/10.1371/journal.pone.0129489},
   keywords = {BDNF; Hypoxia; Pulmonary Artery},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS ONE},
   title = {Role of Hypoxia-Induced Brain Derived Neurotrophic Factor in Human Pulmonary Artery Smooth Muscle},
   url = {http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0129489},
   volume = {10},
   year = {2015}
}

TY  - JOUR
ID  - 1306908
AU  - Hartman, William - Helán, Martin - Smelter, Dan - Sathish, Venkatashelem - Thompson, Michael - Pabelick, Chistina M. - Johnson, Bruce - Prakash, Y.S.
PY  - 2015
TI  - Role of Hypoxia-Induced Brain Derived Neurotrophic Factor in Human Pulmonary Artery Smooth Muscle
JF  - PLOS ONE
VL  - 10
IS  - 7
SP  - "e0129489"
EP  - "e0129489"
PB  - Public Library of Science
SN  - 19326203
KW  - BDNF
KW  - Hypoxia
KW  - Pulmonary Artery
UR  - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0129489
L2  - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0129489
N2  - Hypoxia effects on pulmonary artery structure and function are key to diseases such as pulmonary hypertension. Recent studies suggest that growth factors called neurotrophins, particularly brain-derived neurotrophic factor (BDNF), can influence lung structure and function, and their role in the pulmonary artery warrants further investigation. In this study, we examined the effect of hypoxia on BDNF in humans, and the influence of hypoxiaenhanced BDNF expression and signaling in human pulmonary artery smooth muscle cells (PASMCs). 48h of 1% hypoxia enhanced BDNF and TrkB expression, as well as release of BDNF. In arteries of patients with pulmonary hypertension, BDNF expression and release was higher at baseline. In isolated PASMCs, hypoxia-induced BDNF increased intracellular Ca2+ responses to serotonin: an effect altered by HIF1 inhibition or by neutralization of extracellular BDNF via chimeric TrkB-Fc. Enhanced BDNF/TrkB signaling increased PASMC survival and proliferation, and decreased apoptosis following hypoxia. Enhanced expression and signaling of the BDNF-TrkB system in PASMCs is a potential mechanism by which hypoxia can promote changes in pulmonary artery structure and function. Accordingly, the BDNF-TrkB system could be a key player in the pathogenesis of hypoxia- induced pulmonary vascular diseases, and thus a potential target for therapy.
ER  -

HARTMAN, William; Martin HELÁN; Dan SMELTER; Venkatashelem SATHISH; Michael THOMPSON; Chistina M. PABELICK; Bruce JOHNSON a Y.S. PRAKASH. Role of Hypoxia-Induced Brain Derived Neurotrophic Factor in Human Pulmonary Artery Smooth Muscle. \textit{PLOS ONE}. San Francisco: Public Library of Science, 2015, roč.~10, č.~7, s.~''e0129489'', 17 s. ISSN~1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0129489.

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