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@article{1307898,
author = {Záveský, Luděk and Jandáková, Eva and Turyna, Radovan and Langmeierová, Lucie and Weinberger, Vít and Záveská Drábková, Lenka and Hůlková, Martina and Hořínek, Aleš and Dušková, Daniela and Feyereisl, Jaroslav and Minář, Luboš and Kohoutová, Milada},
article_location = {Dordrecht},
article_number = {4},
doi = {http://dx.doi.org/10.1007/s12253-015-9914-y},
keywords = {ovarian cancer; endometrial cancer; urine; microRNA;miR-92a; miR-106b},
language = {eng},
issn = {1219-4956},
journal = {Pathology & Oncology Research},
title = {Evaluation of Cell-Free Urine microRNAs Expression for the Use in Diagnosis of Ovarian and Endometrial Cancers. A Pilot Study},
url = {http://link.springer.com/article/10.1007%2Fs12253-015-9914-y},
volume = {21},
year = {2015}
}
TY - JOUR
ID - 1307898
AU - Záveský, Luděk - Jandáková, Eva - Turyna, Radovan - Langmeierová, Lucie - Weinberger, Vít - Záveská Drábková, Lenka - Hůlková, Martina - Hořínek, Aleš - Dušková, Daniela - Feyereisl, Jaroslav - Minář, Luboš - Kohoutová, Milada
PY - 2015
TI - Evaluation of Cell-Free Urine microRNAs Expression for the Use in Diagnosis of Ovarian and Endometrial Cancers. A Pilot Study
JF - Pathology & Oncology Research
VL - 21
IS - 4
SP - 1027-1035
EP - 1027-1035
PB - Springer
SN - 12194956
KW - ovarian cancer
KW - endometrial cancer
KW - urine
KW - microRNA;miR-92a
KW - miR-106b
UR - http://link.springer.com/article/10.1007%2Fs12253-015-9914-y
N2 - Among gynaecological cancers, epithelial ovarian cancers are the most deadly cancers while endometrial cancers are the most common diseases. Efforts to establish relevant novel diagnostic, screening and prognostic markers are aimed to help reduce the high level of mortality, chemoresistance and recurrence, particularly in ovarian cancer. MicroRNAs, the class of post-transcriptional regulators, have emerged as the promising diagnostic and prognostic markers associated with various diseased states recently. Urine has been shown as the source of microRNAs several years ago; however, there has been lack of information on urine microRNA expression in ovarian and endometrial cancers till now. In this pilot study, we examined the expression of candidate cell-free urine microRNAs in ovarian cancer and endometrial cancer patients using quantitative real-time PCR. We compared the expression between pre- and post-surgery ovarian cancer samples, and between patients with ovarian and endometrial cancers and healthy controls, within three types of experiments. These experiments evaluated three different isolation methods of urine RNA, representing two supernatant and one exosome fractions of extracellular microRNA. In ovarian cancer, we found miR-92a significantly up-regulated, and miR-106b significantly down-regulated in comparison with control samples. In endometrial cancer, only miR-106b was found down-regulated significantly compared to control samples. Using exosome RNA, no significant de-regulations in microRNAs expression could be found in either of the cancers investigated.We propose that more research should now focus on confirming the diagnostic potential of urine microRNAs in gynaecological cancers using more clinical samples and largescale expression profiling methods.
ER -
ZÁVESKÝ, Luděk, Eva JANDÁKOVÁ, Radovan TURYNA, Lucie LANGMEIEROVÁ, Vít WEINBERGER, Lenka ZÁVESKÁ DRÁBKOVÁ, Martina HŮLKOVÁ, Aleš HOŘÍNEK, Daniela DUŠKOVÁ, Jaroslav FEYEREISL, Luboš MINÁŘ a Milada KOHOUTOVÁ. Evaluation of Cell-Free Urine microRNAs Expression for the Use in Diagnosis of Ovarian and Endometrial Cancers. A Pilot Study. \textit{Pathology \&{} Oncology Research}. Dordrecht: Springer, 2015, roč.~21, č.~4, s.~1027-1035. ISSN~1219-4956. Dostupné z: https://dx.doi.org/10.1007/s12253-015-9914-y.
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