G-quadruplex-based structural transitions in 15-mer DNA
oligonucleotides varying in lengths of internal oligo(dG)
stretches detected by voltammetric techniques

J 2015

G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques

VIDLÁKOVÁ, Pavlína; Hana PIVOŇKOVÁ; Iva KEJNOVSKÁ; Libuše TRNKOVÁ; Michaela VORLÍČKOVÁ et al.

Základní údaje

Originální název

G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques

Autoři

VIDLÁKOVÁ, Pavlína; Hana PIVOŇKOVÁ; Iva KEJNOVSKÁ; Libuše TRNKOVÁ; Michaela VORLÍČKOVÁ; Miroslav FOJTA a Luděk HAVRAN

Vydání

Analytical and Bioanalytical chemistry, HEIDELBERG, SPRINGER HEIDELBERG, 2015, 1618-2642

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.125

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/15:00083572

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Oligonucleotides; Electrochemical methods; G-quadruplex; Hanging mercury drop electrode

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2020 17:44, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Electrochemical methods, particularly when applied in connection with mercury-containing electrodes, are excellent tools for studying nucleic acids structure and monitoring structural transitions. We studied the effect of the length of the central (dG) (n) stretch (varying from 0 to 15 guanine residues) in 15-mer oligodeoxynucleotides (ODN, G0 to G15) on their electrochemical and interfacial behavior at mercury and carbon electrodes. The intensity of guanine oxidation signal at the carbon electrode (peak G(ox)) was observed to increase continuously with number of guanines between 0 and 15, with only a slight positive shift for ODNs with seven or more guanines in the central segment. Very different effects were observed when the peak G(HMDE) was measured at the mercury electrode. Intensity of the latter signal increased with number of guanines up to G5, and decreased sharply with further elongation of the (dG) (n) stretch. CD spectroscopy and electrophoresis experiments revealed formation of parallel intermolecular quadruplex structures for ODNs containing five or more G residues. Further measurements made by cyclic and alternating-current voltammetry revealed a strong influence of the ODN structure on their behavior at electrically charged surfaces.

Citovat

VIDLÁKOVÁ, Pavlína; Hana PIVOŇKOVÁ; Iva KEJNOVSKÁ; Libuše TRNKOVÁ; Michaela VORLÍČKOVÁ; Miroslav FOJTA a Luděk HAVRAN. G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques. Analytical and Bioanalytical chemistry. HEIDELBERG: SPRINGER HEIDELBERG, 2015, roč. 407, č. 19, s. 5817-5826. ISSN 1618-2642. Dostupné z: https://doi.org/10.1007/s00216-015-8768-1.

@article{1308668,
   author = {Vidláková, Pavlína and Pivoňková, Hana and Kejnovská, Iva and Trnková, Libuše and Vorlíčková, Michaela and Fojta, Miroslav and Havran, Luděk},
   article_location = {HEIDELBERG},
   article_number = {19},
   doi = {https://doi.org/10.1007/s00216-015-8768-1},
   keywords = {Oligonucleotides; Electrochemical methods; G-quadruplex; Hanging mercury drop electrode},
   language = {eng},
   issn = {1618-2642},
   journal = {Analytical and Bioanalytical chemistry},
   title = {G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques},
   url = {http://download.springer.com/static/pdf/375/art%253A10.1007%252Fs00216-015-8768-1.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00216-015-8768-1&token2=exp=1439882775~acl=%2Fstatic%2Fpdf%2F375%2Fart%25253A10.1007%25252Fs00216-015-87},
   volume = {407},
   year = {2015}
}

TY  - JOUR
ID  - 1308668
AU  - Vidláková, Pavlína - Pivoňková, Hana - Kejnovská, Iva - Trnková, Libuše - Vorlíčková, Michaela - Fojta, Miroslav - Havran, Luděk
PY  - 2015
TI  - G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques
JF  - Analytical and Bioanalytical chemistry
VL  - 407
IS  - 19
SP  - 5817-5826
EP  - 5817-5826
PB  - SPRINGER HEIDELBERG
SN  - 16182642
KW  - Oligonucleotides
KW  - Electrochemical methods
KW  - G-quadruplex
KW  - Hanging mercury drop electrode
UR  - http://download.springer.com/static/pdf/375/art%253A10.1007%252Fs00216-015-8768-1.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00216-015-8768-1&token2=exp=1439882775~acl=%2Fstatic%2Fpdf%2F375%2Fart%25253A10.1007%25252Fs00216-015-87
L2  - http://download.springer.com/static/pdf/375/art%253A10.1007%252Fs00216-015-8768-1.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00216-015-8768-1&token2=exp=1439882775~acl=%2Fstatic%2Fpdf%2F375%2Fart%25253A10.1007%25252Fs00216-015-87
N2  - Electrochemical methods, particularly when applied in connection with mercury-containing electrodes, are excellent tools for studying nucleic acids structure and monitoring structural transitions. We studied the effect of the length of the central (dG) (n) stretch (varying from 0 to 15 guanine residues) in 15-mer oligodeoxynucleotides (ODN, G0 to G15) on their electrochemical and interfacial behavior at mercury and carbon electrodes. The intensity of guanine oxidation signal at the carbon electrode (peak G(ox)) was observed to increase continuously with number of guanines between 0 and 15, with only a slight positive shift for ODNs with seven or more guanines in the central segment. Very different effects were observed when the peak G(HMDE) was measured at the mercury electrode. Intensity of the latter signal increased with number of guanines up to G5, and decreased sharply with further elongation of the (dG) (n) stretch. CD spectroscopy and electrophoresis experiments revealed formation of parallel intermolecular quadruplex structures for ODNs containing five or more G residues. Further measurements made by cyclic and alternating-current voltammetry revealed a strong influence of the ODN structure on their behavior at electrically charged surfaces.
ER  -

VIDLÁKOVÁ, Pavlína; Hana PIVOŇKOVÁ; Iva KEJNOVSKÁ; Libuše TRNKOVÁ; Michaela VORLÍČKOVÁ; Miroslav FOJTA a Luděk HAVRAN. G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2015, roč.~407, č.~19, s.~5817-5826. ISSN~1618-2642. Dostupné z: https://doi.org/10.1007/s00216-015-8768-1.

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