Telomere dynamics in the lower plant Physcomitrella patens

J 2015

Telomere dynamics in the lower plant Physcomitrella patens

FOJTOVÁ, Miloslava; Eva SÝKOROVÁ; Lucie NAJDEKROVÁ; Pavla POLANSKÁ; Dagmar ZACHOVÁ et. al.

Základní údaje

Originální název

Telomere dynamics in the lower plant Physcomitrella patens

Autoři

FOJTOVÁ, Miloslava (203 Česká republika, garant, domácí); Eva SÝKOROVÁ (203 Česká republika, domácí); Lucie NAJDEKROVÁ (203 Česká republika, domácí); Pavla POLANSKÁ (203 Česká republika, domácí); Dagmar ZACHOVÁ (203 Česká republika, domácí); Radka VÁGNEROVÁ (203 Česká republika); Karel J. ANGELIS (203 Česká republika) a Jiří FAJKUS (203 Česká republika, domácí)

Vydání

Plant Molecular Biology, Springer, Dordrecht (Netherlands), 2015, 0167-4412

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.905

Kód RIV

RIV/00216224:14740/15:00080971

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1007/s11103-015-0299-9

UT WoS

000352086700005

EID Scopus

2-s2.0-84930704160

Klíčová slova anglicky

Physcomitrella patens; Telomere maintenance; Telomerase; PpTERT structure; DSB repair mutants

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 10. 2015 12:48, Martina Prášilová

Anotace

V originále

A comparative approach in biology is needed to assess the universality of rules governing this discipline. In plant telomere research, most of the key principles were established based on studies in only single model plant, Arabidopsis thaliana. These principles include the absence of telomere shortening during plant development and the corresponding activity of telomerase in dividing (meristem) plant cells. Here we examine these principles in Physcomitrella patens as a representative of lower plants. To follow telomerase expression, we first characterize the gene coding for the telomerase reverse transcriptase subunit PpTERT in P. patens, for which only incomplete prediction has been available so far. In protonema cultures of P. patens, growing by filament apical cell division, the proportion of apical (dividing) cells was quantified and telomere length, telomerase expression and activity were determined. Our results show telomere stability and demonstrate proportionality of telomerase activity and expression with the number of apical cells. In addition, we analyze telomere maintenance in mre11, rad50, nbs1, ku70 and lig4 mutants of P. patens and compare the impact of these mutations in double-strand-break (DSB) repair pathways with earlier observations in corresponding A. thaliana mutants. Telomere phenotypes are absent and DSB repair kinetics is not affected in P. patens mutants for DSB factors involved in non-homologous end joining (NHEJ). This is compliant with the overall dominance of homologous recombination over NHEJ pathways in the moss, contrary to the inverse situation in flowering plants.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

EE2.3.20.0189, projekt VaV

Název: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace

GA13-06595S, projekt VaV

Název: Telomery a stabilita genomu u nižších rostlin

Investor: Grantová agentura ČR, Telomery a stabilita genomu u nižších rostlin

Citovat

FOJTOVÁ, Miloslava; Eva SÝKOROVÁ; Lucie NAJDEKROVÁ; Pavla POLANSKÁ; Dagmar ZACHOVÁ; Radka VÁGNEROVÁ; Karel J. ANGELIS a Jiří FAJKUS. Telomere dynamics in the lower plant Physcomitrella patens. Plant Molecular Biology. Springer: Dordrecht (Netherlands), 2015, roč. 87, č. 6, s. 591-601. ISSN 0167-4412. Dostupné z: https://dx.doi.org/10.1007/s11103-015-0299-9.

@article{1309141,
   author = {Fojtová, Miloslava and Sýkorová, Eva and Najdekrová, Lucie and Polanská, Pavla and Zachová, Dagmar and Vágnerová, Radka and Angelis, Karel J. and Fajkus, Jiří},
   article_location = {Springer},
   article_number = {6},
   doi = {http://dx.doi.org/10.1007/s11103-015-0299-9},
   keywords = {Physcomitrella patens; Telomere maintenance; Telomerase; PpTERT structure; DSB repair mutants},
   language = {eng},
   issn = {0167-4412},
   journal = {Plant Molecular Biology},
   title = {Telomere dynamics in the lower plant Physcomitrella patens},
   url = {http://download.springer.com/static/pdf/161/art%253A10.1007%252Fs11103-015-0299-9.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs11103-015-0299-9&token2=exp=1443784026~acl=%2Fstatic%2Fpdf%2F161%2Fart%25253A10.1007%25252Fs11103-015-02},
   volume = {87},
   year = {2015}
}

TY  - JOUR
ID  - 1309141
AU  - Fojtová, Miloslava - Sýkorová, Eva - Najdekrová, Lucie - Polanská, Pavla - Zachová, Dagmar - Vágnerová, Radka - Angelis, Karel J. - Fajkus, Jiří
PY  - 2015
TI  - Telomere dynamics in the lower plant Physcomitrella patens
JF  - Plant Molecular Biology
VL  - 87
IS  - 6
SP  - 591-601
EP  - 591-601
PB  - Dordrecht (Netherlands)
SN  - 01674412
KW  - Physcomitrella patens
KW  - Telomere maintenance
KW  - Telomerase
KW  - PpTERT structure
KW  - DSB repair mutants
UR  - http://download.springer.com/static/pdf/161/art%253A10.1007%252Fs11103-015-0299-9.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs11103-015-0299-9&token2=exp=1443784026~acl=%2Fstatic%2Fpdf%2F161%2Fart%25253A10.1007%25252Fs11103-015-02
L2  - http://download.springer.com/static/pdf/161/art%253A10.1007%252Fs11103-015-0299-9.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs11103-015-0299-9&token2=exp=1443784026~acl=%2Fstatic%2Fpdf%2F161%2Fart%25253A10.1007%25252Fs11103-015-02
N2  - A comparative approach in biology is needed to assess the universality of rules governing this discipline. In plant telomere research, most of the key principles were established based on studies in only single model plant, Arabidopsis thaliana. These principles include the absence of telomere shortening during plant development and the corresponding activity of telomerase in dividing (meristem) plant cells. Here we examine these principles in Physcomitrella patens as a representative of lower plants. To follow telomerase expression, we first characterize the gene coding for the telomerase reverse transcriptase subunit PpTERT in P. patens, for which only incomplete prediction has been available so far. In protonema cultures of P. patens, growing by filament apical cell division, the proportion of apical (dividing) cells was quantified and telomere length, telomerase expression and activity were determined. Our results show telomere stability and demonstrate proportionality of telomerase activity and expression with the number of apical cells. In addition, we analyze telomere maintenance in mre11, rad50, nbs1, ku70 and lig4 mutants of P. patens and compare the impact of these mutations in double-strand-break (DSB) repair pathways with earlier observations in corresponding A. thaliana mutants. Telomere phenotypes are absent and DSB repair kinetics is not affected in P. patens mutants for DSB factors involved in non-homologous end joining (NHEJ). This is compliant with the overall dominance of homologous recombination over NHEJ pathways in the moss, contrary to the inverse situation in flowering plants.
ER  -

FOJTOVÁ, Miloslava; Eva SÝKOROVÁ; Lucie NAJDEKROVÁ; Pavla POLANSKÁ; Dagmar ZACHOVÁ; Radka VÁGNEROVÁ; Karel J. ANGELIS a Jiří FAJKUS. Telomere dynamics in the lower plant Physcomitrella patens. \textit{Plant Molecular Biology}. Springer: Dordrecht (Netherlands), 2015, roč.~87, č.~6, s.~591-601. ISSN~0167-4412. Dostupné z: https://dx.doi.org/10.1007/s11103-015-0299-9.

Přehled o publikaci