Novel Cryptosporidium bat genotypes III and IV in bats from the
USA and Czech Republic

J 2015

Novel Cryptosporidium bat genotypes III and IV in bats from the USA and Czech Republic

KVÁČ, Martin; Anna HOŘICKÁ; Bohumil SAK; Jitka PREDIGER; Jiří SALÁT et al.

Základní údaje

Originální název

Novel Cryptosporidium bat genotypes III and IV in bats from the USA and Czech Republic

Autoři

KVÁČ, Martin; Anna HOŘICKÁ; Bohumil SAK; Jitka PREDIGER; Jiří SALÁT; Jana ŠIRMAROVÁ; Tomáš BARTONIČKA; Mark CLARK; Joba Rose CHELLADURAI; Erin GILLAM a John MCEVOY

Vydání

Parasitology Research, 2015, 0932-0113

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.027

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/15:00083668

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Cryptosporidium Bats SSU Actin PCR

Štítky

AKR, rivok

Změněno: 19. 2. 2018 10:39, doc. Mgr. Tomáš Bartonička, Ph.D.

Anotace

V originále

Bats from the families Rhinolophidae (n=90) and Vespertilionidae (n = 191) in the USA and Czech Republic were screened for the presence of Cryptosporidium by microscopic and molecular analysis of faecal samples collected from rectum of dissected animals and from the ground beneath roosting sites. Cryptosporidium oocysts were not detected in any of the 281 faecal specimens examined using the aniline–carbol– methyl violet staining method. Nested PCR amplification, sequencing and phylogenetic analysis of the small ribosomal subunit rRNA and actin genes were used to identify isolates and infer evolutionary relationships. Cryptosporidium parvum was identified in a western small-footed bat (Myotis ciliolabrum) from the USA and a common pipistrelle bats (Pipistrellus pipistrellus) from the Czech Republic. Two novel genotypes were identified and named Cryptosporidium bat genotype III and IV. Bat genotype III was found in two big brown bats (Eptesicus fuscus) from the USA. Bat genotype IV was detected in two common pipistrelle bats from the Czech Republic.

Citovat

KVÁČ, Martin; Anna HOŘICKÁ; Bohumil SAK; Jitka PREDIGER; Jiří SALÁT; Jana ŠIRMAROVÁ; Tomáš BARTONIČKA; Mark CLARK; Joba Rose CHELLADURAI; Erin GILLAM a John MCEVOY. Novel Cryptosporidium bat genotypes III and IV in bats from the USA and Czech Republic. Parasitology Research. 2015, roč. 114, č. 10, s. 3917-3921. ISSN 0932-0113. Dostupné z: https://doi.org/10.1007/s00436-015-4654-1.

@article{1309680,
   author = {Kváč, Martin and Hořická, Anna and Sak, Bohumil and Prediger, Jitka and Salát, Jiří and Širmarová, Jana and Bartonička, Tomáš and Clark, Mark and Chelladurai, Joba Rose and Gillam, Erin and McEvoy, John},
   article_number = {10},
   doi = {https://doi.org/10.1007/s00436-015-4654-1},
   keywords = {Cryptosporidium Bats SSU Actin PCR},
   language = {eng},
   issn = {0932-0113},
   journal = {Parasitology Research},
   title = {Novel Cryptosporidium bat genotypes III and IV in bats from the USA and Czech Republic},
   volume = {114},
   year = {2015}
}

TY  - JOUR
ID  - 1309680
AU  - Kváč, Martin - Hořická, Anna - Sak, Bohumil - Prediger, Jitka - Salát, Jiří - Širmarová, Jana - Bartonička, Tomáš - Clark, Mark - Chelladurai, Joba Rose - Gillam, Erin - McEvoy, John
PY  - 2015
TI  - Novel Cryptosporidium bat genotypes III and IV in bats from the USA and Czech Republic
JF  - Parasitology Research
VL  - 114
IS  - 10
SP  - 3917-3921
EP  - 3917-3921
SN  - 09320113
KW  - Cryptosporidium Bats SSU Actin PCR
N2  - Bats from the families Rhinolophidae (n=90) and Vespertilionidae (n = 191) in the USA and Czech Republic were screened for the presence of Cryptosporidium by microscopic and molecular analysis of faecal samples collected from rectum of dissected animals and from the ground beneath roosting sites. Cryptosporidium oocysts were not detected in any of the 281 faecal specimens examined using the aniline–carbol– methyl violet staining method. Nested PCR amplification, sequencing and phylogenetic analysis of the small ribosomal subunit rRNA and actin genes were used to identify isolates and infer evolutionary relationships. Cryptosporidium parvum was identified in a western small-footed bat (Myotis ciliolabrum) from the USA and a common pipistrelle bats (Pipistrellus pipistrellus) from the Czech Republic. Two novel genotypes were identified and named Cryptosporidium bat genotype III and IV. Bat genotype III was found in two big brown bats (Eptesicus fuscus) from the USA. Bat genotype IV was detected in two common pipistrelle bats from the Czech Republic.
ER  -

KVÁČ, Martin; Anna HOŘICKÁ; Bohumil SAK; Jitka PREDIGER; Jiří SALÁT; Jana ŠIRMAROVÁ; Tomáš BARTONIČKA; Mark CLARK; Joba Rose CHELLADURAI; Erin GILLAM a John MCEVOY. Novel Cryptosporidium bat genotypes III and IV in bats from the USA and Czech Republic. \textit{Parasitology Research}. 2015, roč.~114, č.~10, s.~3917-3921. ISSN~0932-0113. Dostupné z: https://doi.org/10.1007/s00436-015-4654-1.

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