HEGER, Zbynek, Natalia CERNEI, Sona KRIZKOVA, Michal MASAŘÍK, Pavel KOPEL, Petr HODEK, Ondrej ZITKA, Vojtech ADAM a Rene KIZEK. Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection. Scientific Reports. London: Nature Publishing Group, 2015, roč. 5, "8868", s. 1-7. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/srep08868. |
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@article{1311819, author = {Heger, Zbynek and Cernei, Natalia and Krizkova, Sona and Masařík, Michal and Kopel, Pavel and Hodek, Petr and Zitka, Ondrej and Adam, Vojtech and Kizek, Rene}, article_location = {London}, article_number = {"8868"}, doi = {http://dx.doi.org/10.1038/srep08868}, keywords = {Prostate-cancer progression; Urine samples; Diagnostics; Carcinoma}, language = {eng}, issn = {2045-2322}, journal = {Scientific Reports}, title = {Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection}, volume = {5}, year = {2015} }
TY - JOUR ID - 1311819 AU - Heger, Zbynek - Cernei, Natalia - Krizkova, Sona - Masařík, Michal - Kopel, Pavel - Hodek, Petr - Zitka, Ondrej - Adam, Vojtech - Kizek, Rene PY - 2015 TI - Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection JF - Scientific Reports VL - 5 IS - "8868" SP - 1-7 EP - 1-7 PB - Nature Publishing Group SN - 20452322 KW - Prostate-cancer progression KW - Urine samples KW - Diagnostics KW - Carcinoma N2 - Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Forster resonance energy transfer (FRET). The FRET biosensor employs anti-sarcosine antibodies immobilized on paramagnetic nanoparticles surface for specific antigen binding. Successful binding of sarcosine leads to assembly of a sandwich construct composed of anti-sarcosine antibodies keeping the Forster distance (Ro) of FRET pair in required proximity. The detection is based on spectral overlap between gold-functionalized green fluorescent protein and antibodies@quantum dots bioconjugate (lambda(ex) 400 nm). The saturation curve of sarcosine based on FRET efficiency (F-604/F-510 ratio) was tested within linear dynamic range from 5 to 50 nM with detection limit down to 50 pM. Assembled biosensor was then successfully employed for sarcosine quantification in prostatic cell lines (PC3, 22Rv1, PNT1A), and urinary samples of prostate adenocarcinoma patients. ER -
HEGER, Zbynek, Natalia CERNEI, Sona KRIZKOVA, Michal MASAŘÍK, Pavel KOPEL, Petr HODEK, Ondrej ZITKA, Vojtech ADAM a Rene KIZEK. Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection. \textit{Scientific Reports}. London: Nature Publishing Group, 2015, roč.~5, ''8868'', s.~1-7. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/srep08868.
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