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@article{1311838,
author = {Heger, Zbynek and Gumulec, Jaromír and Cernei, Natalia and Tmejova, Katerina and Kopel, Pavel and Balvan, Jan and Masařík, Michal and Zitka, Ondrej and Beklova, Miroslava and Adam, Vojtech and Kizek, Rene},
article_location = {Athens},
article_number = {2},
doi = {http://dx.doi.org/10.3892/or.2014.3627},
keywords = {antisense therapy; delivery; glutathione; liposome; malondialdehyde; metallothionein},
language = {eng},
issn = {1021-335X},
journal = {Oncology Reports},
title = {17 beta-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line},
volume = {33},
year = {2015}
}
TY - JOUR
ID - 1311838
AU - Heger, Zbynek - Gumulec, Jaromír - Cernei, Natalia - Tmejova, Katerina - Kopel, Pavel - Balvan, Jan - Masařík, Michal - Zitka, Ondrej - Beklova, Miroslava - Adam, Vojtech - Kizek, Rene
PY - 2015
TI - 17 beta-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line
JF - Oncology Reports
VL - 33
IS - 2
SP - 921-929
EP - 921-929
PB - Spandidos Publications
SN - 1021335X
KW - antisense therapy
KW - delivery
KW - glutathione
KW - liposome
KW - malondialdehyde
KW - metallothionein
N2 - The present study suggests and describes the application of a delivery system for antisense oligonucleotides against mRNA encoding estrogen receptor proteins alpha and beta. The delivery system is composed of a cationic liposome envelope containing 17 beta-estradiol (E-2) in its structure. Cationic liposomes protect cargo against the extracellular matrix, and E-2 can increase its shuttling efficiency into cells. Using MCF-7 cells derived from estrogen receptor-positive ductal carcinoma, treatment with liposomes against ER alpha was found to decrease MCF-7 proliferation, and importantly the application of both the antisense against ER alpha and beta exhibited an antiproliferative effect expressed as cell viability. Using qRT-PCR, it was shown that MTIA,NF-kappa B1 and K-ras genes, but not TFF1, were downregulated using E-2-based liposomes (evaluated at P=0.05). Further indicators of oxidative stress were employed to assess the effect on treatment efficiency. Glutathione (GSH/GSSG redox ratio), metallothionein (MT) and malondialdehyde (MDA) confirmed a positive effect of antisense therapy resulting in their decreased levels in the MCF-7 cells. Based on these data, we suggest that E2-based liposomes offer sufficient transfer efficiency and moreover, due to the effect on NF-kappa BI, MT and GSH, tumor cells can be chemosensitized to increase treatment effectiveness.
ER -
HEGER, Zbynek, Jaromír GUMULEC, Natalia CERNEI, Katerina TMEJOVA, Pavel KOPEL, Jan BALVAN, Michal MASAŘÍK, Ondrej ZITKA, Miroslava BEKLOVA, Vojtech ADAM a Rene KIZEK. 17 beta-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line. \textit{Oncology Reports}. Athens: Spandidos Publications, 2015, roč.~33, č.~2, s.~921-929. ISSN~1021-335X. Dostupné z: https://dx.doi.org/10.3892/or.2014.3627.
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