Atomic force microscopy for characterization of biomolecules,
affinity complexes and cells

D 2015

Atomic force microscopy for characterization of biomolecules, affinity complexes and cells

SKLÁDAL, Petr, Jan PŘIBYL, Veronika HORŇÁKOVÁ, Patrik GEREG, Zdenka FOHLEROVÁ et. al.

Základní údaje

Originální název

Atomic force microscopy for characterization of biomolecules, affinity complexes and cells

Autoři

SKLÁDAL, Petr (203 Česká republika, garant, domácí), Jan PŘIBYL (203 Česká republika, domácí), Veronika HORŇÁKOVÁ (203 Česká republika, domácí), Patrik GEREG (703 Slovensko, domácí), Zdenka FOHLEROVÁ (203 Česká republika, domácí), Martin JAKUBEC (203 Česká republika, domácí), Zdeněk FARKA (203 Česká republika, domácí), David KOVÁŘ (203 Česká republika, domácí) a Martin PEŠL (203 Česká republika, domácí)

Vydání

Brno, XV. Workshop of Physical Chemists and Electrochemists, od s. 38-39, 2 s. 2015

Nakladatel

Masaryk University

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Kód RIV

RIV/00216224:14740/15:00084143

Organizační jednotka

Středoevropský technologický institut

ISBN

978-80-210-7857-4

Klíčová slova anglicky

atomic force microscopy; force-distance curves; biomolecular interactions

Příznaky

Mezinárodní význam

Změněno: 21. 5. 2018 12:44, MUDr. Martin Pešl, Ph.D.

Anotace

V originále

The instrumentation and services available at the Core Facility of Nanobiotechnology in CEITEC MU will be introduced. The research possibilities will be demonstrated on experiments with proteins, nucleic acids, their affinity complexes and cells. The interaction of ssDNA binding protein with oligonucleotides was imaged using bare tips (Fig. 1), the binding forces in the affinity complex were studied using the protein ligand-modified tip and the ForceRobot AFM head for automated recording of force-distance curves. Similar experiments characterised immunoreactions between antibody and serum albumin and microbial cells as antigens and hybridisation of nucleic acids; interactions were confirmed using surface plasmon resonance and electrochemical measurements. Properties of mast cells were characterised in relation to the changes accompanying biotransformation events (Fig. 2). Simultaneous recording of contractions and electric activity of cardiomyocytes was followed using the AFM cantilever with conductive tip functioning as nanomechanical transducer for cellular biosensor suitable for evaluation of physiologically active compounds in real time.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

SKLÁDAL, Petr, Jan PŘIBYL, Veronika HORŇÁKOVÁ, Patrik GEREG, Zdenka FOHLEROVÁ, Martin JAKUBEC, Zdeněk FARKA, David KOVÁŘ a Martin PEŠL. Atomic force microscopy for characterization of biomolecules, affinity complexes and cells. Online. In Libuše Trnková. XV. Workshop of Physical Chemists and Electrochemists. Brno: Masaryk University, 2015, s. 38-39. ISBN 978-80-210-7857-4.

@inproceedings{1313155,
   author = {Skládal, Petr and Přibyl, Jan and Horňáková, Veronika and Gereg, Patrik and Fohlerová, Zdenka and Jakubec, Martin and Farka, Zdeněk and Kovář, David and Pešl, Martin},
   address = {Brno},
   booktitle = {XV. Workshop of Physical Chemists and Electrochemists},
   editor = {Libuše Trnková},
   keywords = {atomic force microscopy; force-distance curves; biomolecular interactions},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-210-7857-4},
   pages = {38-39},
   publisher = {Masaryk University},
   title = {Atomic force microscopy for characterization of biomolecules, affinity complexes and cells},
   url = {http://www.sci.muni.cz/~labifel/files/soubory/sborn%C3%ADk_2015.pdf#page=38},
   year = {2015}
}

TY  - JOUR
ID  - 1313155
AU  - Skládal, Petr - Přibyl, Jan - Horňáková, Veronika - Gereg, Patrik - Fohlerová, Zdenka - Jakubec, Martin - Farka, Zdeněk - Kovář, David - Pešl, Martin
PY  - 2015
TI  - Atomic force microscopy for characterization of biomolecules, affinity complexes and cells
PB  - Masaryk University
CY  - Brno
SN  - 9788021078574
KW  - atomic force microscopy
KW  - force-distance curves
KW  - biomolecular interactions
UR  - http://www.sci.muni.cz/~labifel/files/soubory/sborn%C3%ADk_2015.pdf#page=38
N2  - The instrumentation and services available at the Core Facility of Nanobiotechnology in CEITEC MU will be introduced. The research possibilities will be demonstrated on experiments with proteins, nucleic acids, their affinity complexes and cells. The interaction of ssDNA binding protein with oligonucleotides was imaged using bare tips (Fig. 1), the binding forces in the affinity complex were studied using the protein ligand-modified tip and the ForceRobot AFM head for automated recording of force-distance curves. Similar experiments characterised immunoreactions between antibody and serum albumin and microbial cells as antigens and hybridisation of nucleic acids; interactions were confirmed using surface plasmon resonance and electrochemical measurements. Properties of mast cells were characterised in relation to the changes accompanying biotransformation events (Fig. 2). Simultaneous recording of contractions and electric activity of cardiomyocytes was followed using the AFM cantilever with conductive tip functioning as nanomechanical transducer for cellular biosensor suitable for evaluation of physiologically active compounds in real time.
ER  -

SKLÁDAL, Petr, Jan PŘIBYL, Veronika HORŇÁKOVÁ, Patrik GEREG, Zdenka FOHLEROVÁ, Martin JAKUBEC, Zdeněk FARKA, David KOVÁŘ a Martin PEŠL. Atomic force microscopy for characterization of biomolecules, affinity complexes and cells. Online. In Libuše Trnková. \textit{XV. Workshop of Physical Chemists and Electrochemists}. Brno: Masaryk University, 2015, s.~38-39. ISBN~978-80-210-7857-4.

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