Detection of Bim and Puma in mouse hair follicles using
immunofluorescence and TUNEL assay double staining.

J 2015

Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining.

VESELÁ, Barbora a Eva MATALOVÁ

Základní údaje

Originální název

Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining.

Autoři

VESELÁ, Barbora a Eva MATALOVÁ

Vydání

Biotechnic & Histochemistry, England, BIOS Scientific Publishers, 2015, 1052-0295

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.078

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/15:00114959

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

apoptosis; Bim; development; hair follicle; immunofluorescence; Puma; TUNEL

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 6. 2020 15:02, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Apoptosis in hair follicles often is studied under pathological conditions; little is known about apoptotic mechanisms during normal hair follicle formation and maintenance. We investigated proteins of intrinsic apoptotic pathway, Bim and Puma, during hair follicle development and the first catagen stage using immunofluorescence to describe their expression patterns and to correlate them with apoptosis as determined by TUNEL assay. Both proteins were found in developing follicles. Bim and Puma overlapped apoptosis only partially during physiological apoptotic stage and they were present in non-apoptotic parts of the follicles. Our findings suggest that these primary apoptotic molecules participate in postnatal development and maintenance of hair follicles.

Citovat

VESELÁ, Barbora a Eva MATALOVÁ. Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining. Biotechnic & Histochemistry. England: BIOS Scientific Publishers, 2015, roč. 90, č. 8, s. 587-593. ISSN 1052-0295. Dostupné z: https://doi.org/10.3109/10520295.2015.1062916.

@article{1313265,
   author = {Veselá, Barbora and Matalová, Eva},
   article_location = {England},
   article_number = {8},
   doi = {https://doi.org/10.3109/10520295.2015.1062916},
   keywords = {apoptosis; Bim; development; hair follicle; immunofluorescence; Puma; TUNEL},
   language = {eng},
   issn = {1052-0295},
   journal = {Biotechnic & Histochemistry},
   title = {Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining.},
   url = {http://www.tandfonline.com/doi/abs/10.3109/10520295.2015.1062916?journalCode=ibih20#.VhZlLm5iPEE},
   volume = {90},
   year = {2015}
}

TY  - JOUR
ID  - 1313265
AU  - Veselá, Barbora - Matalová, Eva
PY  - 2015
TI  - Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining.
JF  - Biotechnic & Histochemistry
VL  - 90
IS  - 8
SP  - 587-593
EP  - 587-593
PB  - BIOS Scientific Publishers
SN  - 10520295
KW  - apoptosis
KW  - Bim
KW  - development
KW  - hair follicle
KW  - immunofluorescence
KW  - Puma
KW  - TUNEL
UR  - http://www.tandfonline.com/doi/abs/10.3109/10520295.2015.1062916?journalCode=ibih20#.VhZlLm5iPEE
L2  - http://www.tandfonline.com/doi/abs/10.3109/10520295.2015.1062916?journalCode=ibih20#.VhZlLm5iPEE
N2  - Apoptosis in hair follicles often is studied under pathological conditions; little is known about apoptotic mechanisms during normal hair follicle formation and maintenance. We investigated proteins of intrinsic apoptotic pathway, Bim and Puma, during hair follicle development and the first catagen stage using immunofluorescence to describe their expression patterns and to correlate them with apoptosis as determined by TUNEL assay. Both proteins were found in developing follicles. Bim and Puma overlapped apoptosis only partially during physiological apoptotic stage and they were present in non-apoptotic parts of the follicles. Our findings suggest that these primary apoptotic molecules participate in postnatal development and maintenance of hair follicles.
ER  -

VESELÁ, Barbora a Eva MATALOVÁ. Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining. \textit{Biotechnic \&{} Histochemistry}. England: BIOS Scientific Publishers, 2015, roč.~90, č.~8, s.~587-593. ISSN~1052-0295. Dostupné z: https://doi.org/10.3109/10520295.2015.1062916.

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