Root and Eruption Defects in c-Fos Mice Are Driven by Loss of
Osteoclasts

J 2015

Root and Eruption Defects in c-Fos Mice Are Driven by Loss of Osteoclasts

ALFAQEEH, Sarah; Veronika ORALOVÁ; Morwenna FOXWORTHY; Eva MATALOVÁ; Aganemnon GRIGORIADIS et al.

Základní údaje

Originální název

Root and Eruption Defects in c-Fos Mice Are Driven by Loss of Osteoclasts

Autoři

ALFAQEEH, Sarah; Veronika ORALOVÁ; Morwenna FOXWORTHY; Eva MATALOVÁ; Aganemnon GRIGORIADIS a Abigail TUCKER

Vydání

Journal of Dental Research, SAGE PUBLICATIONS INC, 2015, 0022-0345

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30208 Dentistry, oral surgery and medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.602

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/15:00113937

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

tooth; bone; rescue; HERS; osteopetrosis; remodeling

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 6. 2020 08:39, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

c-Fos homozygous mice lack osteoclasts with a failure of the teeth to erupt and with an arrest of root development. Here, we characterize the defects associated with the failure in root development and the loss of the tooth-bone interface, and we investigate the underlying causes. We show that, while homozygous c-Fos mice have no multinucleated osteoclasts, heterozygous mice have a reduction in the number of osteoclasts with a reduction in the tooth-bone interface during development and subtle skeletal defects postnatally. In the homozygous mutants bone is found to penetrate the tooth, particularly at the apical end, physically disrupting the root forming HERS (Hertwig's epithelial root sheath) cells. The cells of the HERS continue to proliferate but cannot extend downward due to the presence of bone, leading to a loss of root formation. Tooth germ culture showed that the developing tooth invaded the static bone in mutant tissue, rather than the bone encroaching on the tooth. Although c-Fos has been shown to be expressed in developing teeth, the defect in maintenance of the tooth-bone interface appears to be driven solely by the lack of osteoclasts, as this defect can be rescued in the presence of donor osteoclasts. The rescue suggests that signals from the tooth recruit osteoclasts to clear the bone from around the tooth, allowing the tooth to grow, form roots, and later erupt.

Návaznosti

GB14-37368G, projekt VaV

Název: Centrum orofaciálního vývoje a regenerace

Investor: Grantová agentura ČR, Centrum orofaciálního vývoje a regenerace

Citovat

ALFAQEEH, Sarah; Veronika ORALOVÁ; Morwenna FOXWORTHY; Eva MATALOVÁ; Aganemnon GRIGORIADIS a Abigail TUCKER. Root and Eruption Defects in c-Fos Mice Are Driven by Loss of Osteoclasts. Journal of Dental Research. SAGE PUBLICATIONS INC, 2015, roč. 94, č. 12, s. 1724-1731. ISSN 0022-0345. Dostupné z: https://doi.org/10.1177/0022034515608828.

@article{1313312,
   author = {Alfaqeeh, Sarah and Oralová, Veronika and Foxworthy, Morwenna and Matalová, Eva and Grigoriadis, Aganemnon and Tucker, Abigail},
   article_number = {12},
   doi = {https://doi.org/10.1177/0022034515608828},
   keywords = {tooth; bone; rescue; HERS; osteopetrosis; remodeling},
   language = {eng},
   issn = {0022-0345},
   journal = {Journal of Dental Research},
   title = {Root and Eruption Defects in c-Fos Mice Are Driven by Loss of Osteoclasts},
   url = {https://doi.org/10.1177/0022034515608828},
   volume = {94},
   year = {2015}
}

TY  - JOUR
ID  - 1313312
AU  - Alfaqeeh, Sarah - Oralová, Veronika - Foxworthy, Morwenna - Matalová, Eva - Grigoriadis, Aganemnon - Tucker, Abigail
PY  - 2015
TI  - Root and Eruption Defects in c-Fos Mice Are Driven by Loss of Osteoclasts
JF  - Journal of Dental Research
VL  - 94
IS  - 12
SP  - 1724-1731
EP  - 1724-1731
PB  - SAGE PUBLICATIONS INC
SN  - 00220345
KW  - tooth
KW  - bone
KW  - rescue
KW  - HERS
KW  - osteopetrosis
KW  - remodeling
UR  - https://doi.org/10.1177/0022034515608828
L2  - https://doi.org/10.1177/0022034515608828
N2  - c-Fos homozygous mice lack osteoclasts with a failure of the teeth to erupt and with an arrest of root development. Here, we characterize the defects associated with the failure in root development and the loss of the tooth-bone interface, and we investigate the underlying causes. We show that, while homozygous c-Fos mice have no multinucleated osteoclasts, heterozygous mice have a reduction in the number of osteoclasts with a reduction in the tooth-bone interface during development and subtle skeletal defects postnatally. In the homozygous mutants bone is found to penetrate the tooth, particularly at the apical end, physically disrupting the root forming HERS (Hertwig's epithelial root sheath) cells. The cells of the HERS continue to proliferate but cannot extend downward due to the presence of bone, leading to a loss of root formation. Tooth germ culture showed that the developing tooth invaded the static bone in mutant tissue, rather than the bone encroaching on the tooth. Although c-Fos has been shown to be expressed in developing teeth, the defect in maintenance of the tooth-bone interface appears to be driven solely by the lack of osteoclasts, as this defect can be rescued in the presence of donor osteoclasts. The rescue suggests that signals from the tooth recruit osteoclasts to clear the bone from around the tooth, allowing the tooth to grow, form roots, and later erupt.
ER  -

ALFAQEEH, Sarah; Veronika ORALOVÁ; Morwenna FOXWORTHY; Eva MATALOVÁ; Aganemnon GRIGORIADIS a Abigail TUCKER. Root and Eruption Defects in c-Fos Mice Are Driven by Loss of Osteoclasts. \textit{Journal of Dental Research}. SAGE PUBLICATIONS INC, 2015, roč.~94, č.~12, s.~1724-1731. ISSN~0022-0345. Dostupné z: https://doi.org/10.1177/0022034515608828.

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