Osteogenic Profile of Mesenchymal Cell Populations Contributing
to Alveolar Bone Formation

J 2015

Osteogenic Profile of Mesenchymal Cell Populations Contributing to Alveolar Bone Formation

MINAŘÍKOVÁ, Monika; Veronika ORALOVÁ; Barbora VESELÁ; Ralf RADLANSKI; Eva MATALOVÁ et al.

Základní údaje

Originální název

Osteogenic Profile of Mesenchymal Cell Populations Contributing to Alveolar Bone Formation

Autoři

MINAŘÍKOVÁ, Monika; Veronika ORALOVÁ; Barbora VESELÁ; Ralf RADLANSKI a Eva MATALOVÁ

Vydání

Cells Tissues Organs, Basel, KARGER, 2015, 1422-6405

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.228

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Dental and peridental mesenchymal cells; Dental follicle; Odontogenesis; Osteogenesis; PCR Arrays; 3D images

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 6. 2020 14:53, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Teeth develop within the surrounding periodontal tissues, involving the alveolar bone, periodontal ligament and cementum. The alveolar bone originates through the process of intramembranous ossification involving mesenchymal cells from the tooth germ. As most available data are related to endochondral ossification, we examined the molecular background of alveolar bone development. We investigated the osteogenic profile of mesenchymal cells dissected from mouse mandible slices at the stage of early alveolar bone formation. Relative monitoring of gene expression was undertaken using PCR Arrays; this included the profiles of 84 genes associated with osteogenesis. To examine the tooth-bone interface, stages with detectable changes in bone remodelling during development (E13.0, E14.0 and E15.0) were chosen and compared with each other. These results showed a statistically significant increase in the expression of the genes Fgf3, Ctsk, Icam-1, Mmp9, Itga3 and Tuft1, and of a wide range of collagens (Col1a2, Col3a1, Col7a1, Col12a1, Col14a1). Decreased expression was detected in the case of Col2a1, Sox9, Smad2 and Vegfb. To confirm these changes in gene expression, immunofluorescence analyses of Mmp9 and Sox9 proteins were performed in situ. Our research has identified several candidate genes that may be crucial for the initiation of alveolar bone formation and is the basis for further functional studies.

Návaznosti

GB14-37368G, projekt VaV

Název: Centrum orofaciálního vývoje a regenerace

Investor: Grantová agentura ČR, Centrum orofaciálního vývoje a regenerace

Citovat

MINAŘÍKOVÁ, Monika; Veronika ORALOVÁ; Barbora VESELÁ; Ralf RADLANSKI a Eva MATALOVÁ. Osteogenic Profile of Mesenchymal Cell Populations Contributing to Alveolar Bone Formation. Cells Tissues Organs. Basel: KARGER, 2015, roč. 200, č. 5, s. 339-348. ISSN 1422-6405. Dostupné z: https://doi.org/10.1159/000439165.

@article{1313660,
   author = {Minaříková, Monika and Oralová, Veronika and Veselá, Barbora and Radlanski, Ralf and Matalová, Eva},
   article_location = {Basel},
   article_number = {5},
   doi = {https://doi.org/10.1159/000439165},
   keywords = {Dental and peridental mesenchymal cells; Dental follicle; Odontogenesis; Osteogenesis; PCR Arrays; 3D images},
   language = {eng},
   issn = {1422-6405},
   journal = {Cells Tissues Organs},
   title = {Osteogenic Profile of Mesenchymal Cell Populations Contributing to Alveolar Bone Formation},
   url = {http://dx.doi.org/10.1159/000439165},
   volume = {200},
   year = {2015}
}

TY  - JOUR
ID  - 1313660
AU  - Minaříková, Monika - Oralová, Veronika - Veselá, Barbora - Radlanski, Ralf - Matalová, Eva
PY  - 2015
TI  - Osteogenic Profile of Mesenchymal Cell Populations Contributing to Alveolar Bone Formation
JF  - Cells Tissues Organs
VL  - 200
IS  - 5
SP  - 339-348
EP  - 339-348
PB  - KARGER
SN  - 14226405
KW  - Dental and peridental mesenchymal cells
KW  - Dental follicle
KW  - Odontogenesis
KW  - Osteogenesis
KW  - PCR Arrays
KW  - 3D images
UR  - http://dx.doi.org/10.1159/000439165
L2  - http://dx.doi.org/10.1159/000439165
N2  - Teeth develop within the surrounding periodontal tissues, involving the alveolar bone, periodontal ligament and cementum. The alveolar bone originates through the process of intramembranous ossification involving mesenchymal cells from the tooth germ. As most available data are related to endochondral ossification, we examined the molecular background of alveolar bone development. We investigated the osteogenic profile of mesenchymal cells dissected from mouse mandible slices at the stage of early alveolar bone formation. Relative monitoring of gene expression was undertaken using PCR Arrays; this included the profiles of 84 genes associated with osteogenesis. To examine the tooth-bone interface, stages with detectable changes in bone remodelling during development (E13.0, E14.0 and E15.0) were chosen and compared with each other. These results showed a statistically significant increase in the expression of the genes Fgf3, Ctsk, Icam-1, Mmp9, Itga3 and Tuft1, and of a wide range of collagens (Col1a2, Col3a1, Col7a1, Col12a1, Col14a1). Decreased expression was detected in the case of Col2a1, Sox9, Smad2 and Vegfb. To confirm these changes in gene expression, immunofluorescence analyses of Mmp9 and Sox9 proteins were performed in situ. Our research has identified several candidate genes that may be crucial for the initiation of alveolar bone formation and is the basis for further functional studies.
ER  -

MINAŘÍKOVÁ, Monika; Veronika ORALOVÁ; Barbora VESELÁ; Ralf RADLANSKI a Eva MATALOVÁ. Osteogenic Profile of Mesenchymal Cell Populations Contributing to Alveolar Bone Formation. \textit{Cells Tissues Organs}. Basel: KARGER, 2015, roč.~200, č.~5, s.~339-348. ISSN~1422-6405. Dostupné z: https://doi.org/10.1159/000439165.

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