2014
Award for the best presentations in the category of BSc/MSc students
ŠKORPÍKOVÁ, LucieZákladní údaje
Originální název
Award for the best presentations in the category of BSc/MSc students
Název česky
Cena za nejlepší prezentace v kategorii Bc./Mgr. studentů
Autoři
Vydání
2014
Další údaje
Jazyk
angličtina
Typ výsledku
Ocenění vědecké a umělecké činnosti
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ne
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
Trichinella; multi cystatin-like domain gene; Trichinella spiralis; Trichinella britovi; Trichinella pseudospiralis
Změněno: 13. 10. 2015 11:26, Mgr. Lucie Škorpíková, Ph.D.
Anotace
V originále
The nematodes of the genus Trichinella are intracellular parasites of small intestine and skeletal muscle cells. They can infect a broad range of worldwide distributed hosts like birds, reptiles and mammals. All Trichinella spp. are zoonotic but only six of twelve genotypes have been detected in humans, causing the trichinellosis - a serious, sometimes fatal, human disease, which has been documented in 55 countries of the World. Our study is focused on characterization of composition of excretory-secretory products (ESP) from L1 muscle larvae of three trichinella species - Trichinella spiralis, T. britovi and T. pseudospiralis. The dominant components of L1 ESP are cystatins. These proteins are a reversible inhibitors of proteolytics enzymes - cystein peptideases and they are involved in many relevant biological processes such as development, growth, digestion and migration of parasites and suppression of host immune responses. The main aim of our study is to prepare the recombinant form of three trichinella cystatin and compare/characterize their properties. We used primers specifically designed for identified “multi cystatin-like domain” gene of T. spiralis. Subsequently, we obtained sequences of this gene for the other two species and we compared these data. We found out that cystatin genes of T. spiralis and T. britovi have high sequence homology although gene of T. pseudospiralis differs more (e.g. number of domains, conserved motifs).
Návaznosti
| MUNI/A/0888/2013, interní kód MU |
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