| BALVAN, Jan, Aneta KRIZOVA, Jaromír GUMULEC, Martina RAUDENSKÁ, Zbysek SLADEK, Miroslava SEDLÁČKOVÁ, Petr BABULA, Markéta SZTALMACHOVÁ, Rene KIZEK, Radim CHMELIK a Michal MASAŘÍK. Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis. Plos One. San Francisco: Public Library of Science, 2015, roč. 10, č. 3, s. "e0121674", 16 s. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0121674. |
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@article{1315366,
author = {Balvan, Jan and Krizova, Aneta and Gumulec, Jaromír and Raudenská, Martina and Sladek, Zbysek and Sedláčková, Miroslava and Babula, Petr and Sztalmachová, Markéta and Kizek, Rene and Chmelik, Radim and Masařík, Michal},
article_location = {San Francisco},
article_number = {3},
doi = {http://dx.doi.org/10.1371/journal.pone.0121674},
keywords = {CELL-DEATH; DIGITAL HOLOGRAPHY; PROSTATE-CANCER; NECROSIS; COHERENCE; INTERFEROMETRY; DYNAMICS; EXPOSURE; SYSTEM; CYCLE},
language = {eng},
issn = {1932-6203},
journal = {Plos One},
title = {Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis},
volume = {10},
year = {2015}
}
TY - JOUR
ID - 1315366
AU - Balvan, Jan - Krizova, Aneta - Gumulec, Jaromír - Raudenská, Martina - Sladek, Zbysek - Sedláčková, Miroslava - Babula, Petr - Sztalmachová, Markéta - Kizek, Rene - Chmelik, Radim - Masařík, Michal
PY - 2015
TI - Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis
JF - Plos One
VL - 10
IS - 3
SP - "e0121674"
EP - "e0121674"
PB - Public Library of Science
SN - 19326203
KW - CELL-DEATH
KW - DIGITAL HOLOGRAPHY
KW - PROSTATE-CANCER
KW - NECROSIS
KW - COHERENCE
KW - INTERFEROMETRY
KW - DYNAMICS
KW - EXPOSURE
KW - SYSTEM
KW - CYCLE
N2 - Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection - "Multimodal holographic microscopy (MHM)". The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI-phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis.
ER -
BALVAN, Jan, Aneta KRIZOVA, Jaromír GUMULEC, Martina RAUDENSKÁ, Zbysek SLADEK, Miroslava SEDLÁČKOVÁ, Petr BABULA, Markéta SZTALMACHOVÁ, Rene KIZEK, Radim CHMELIK a Michal MASAŘÍK. Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis. \textit{Plos One}. San Francisco: Public Library of Science, 2015, roč.~10, č.~3, s.~''e0121674'', 16 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0121674.
|
