Auxin-binding pocket of ABP1 is crucial for its
gain-of-function cellular and developmental roles

J 2015

Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles

GRONES, Peter; Xu CHEN; Sibu SIMON; Walter A. KAUFMANN; Riet DE RYCKE et. al.

Základní údaje

Originální název

Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles

Autoři

GRONES, Peter (56 Belgie); Xu CHEN (56 Belgie); Sibu SIMON (56 Belgie); Walter A. KAUFMANN (56 Belgie); Riet DE RYCKE (56 Belgie); Tomasz NODZYNSKI (616 Polsko, garant, domácí); Eva ZAŽÍMALOVÁ (203 Česká republika) a Jiří FRIML (203 Česká republika)

Vydání

Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2015, 0022-0957

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.677

Kód RIV

RIV/00216224:14740/15:00081165

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1093/jxb/erv177

UT WoS

000359688300017

EID Scopus

2-s2.0-84935499521

Klíčová slova anglicky

Auxin; ABP1; Auxin binding; Site-directed mutagenesis; PIN proteins

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 4. 2016 13:36, Mgr. Eva Špillingová

Anotace

V originále

The plant hormone auxin is a key regulator of plant growth and development. Auxin levels are sensed and interpreted by distinct receptor systems that activate a broad range of cellular responses. The Auxin-Binding Protein1 (ABP1) that has been identified based on its ability to bind auxin with high affinity is a prime candidate for the extracellular receptor responsible for mediating a range of auxin effects, in particular, the fast non-transcriptional ones. Contradictory genetic studies suggested prominent or no importance of ABP1 in many developmental processes. However, how crucial the role of auxin binding to ABP1 is for its functions has not been addressed. Here, we show that the auxinbinding pocket of ABP1 is essential for its gain-of-function cellular and developmental roles. In total, 16 different abp1 mutants were prepared that possessed substitutions in the metal core or in the hydrophobic amino acids of the auxin-binding pocket as well as neutral mutations. Their analysis revealed that an intact auxin-binding pocket is a prerequisite for ABP1 to activate downstream components of the ABP1 signalling pathway, such as Rho of Plants (ROPs) and to mediate the clathrin association with membranes for endocytosis regulation. In planta analyses demonstrated the importance of the auxin binding pocket for all known ABP1-mediated postembryonic developmental processes, including morphology of leaf epidermal cells, root growth and root meristem activity, and vascular tissue differentiation. Taken together, these findings suggest that auxin binding to ABP1 is central to its function, supporting the role of ABP1 as auxin receptor.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GA13-40637S, projekt VaV

Název: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Grantová agentura ČR, Genetické studie k identifikaci molekulárních mechanizmu bunecné polarity a auxinového transportu v rostlinách

Citovat

GRONES, Peter; Xu CHEN; Sibu SIMON; Walter A. KAUFMANN; Riet DE RYCKE; Tomasz NODZYNSKI; Eva ZAŽÍMALOVÁ a Jiří FRIML. Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. Journal of Experimental Botany. Oxford: OXFORD UNIV PRESS, 2015, roč. 66, č. 16, s. 5055-5065. ISSN 0022-0957. Dostupné z: https://dx.doi.org/10.1093/jxb/erv177.

@article{1315668,
   author = {Grones, Peter and Chen, Xu and Simon, Sibu and Kaufmann, Walter A. and De Rycke, Riet and Nodzynski, Tomasz and Zažímalová, Eva and Friml, Jiří},
   article_location = {Oxford},
   article_number = {16},
   doi = {http://dx.doi.org/10.1093/jxb/erv177},
   keywords = {Auxin; ABP1; Auxin binding; Site-directed mutagenesis; PIN proteins},
   language = {eng},
   issn = {0022-0957},
   journal = {Journal of Experimental Botany},
   title = {Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles},
   url = {http://jxb.oxfordjournals.org/content/66/16/5055.abstract},
   volume = {66},
   year = {2015}
}

TY  - JOUR
ID  - 1315668
AU  - Grones, Peter - Chen, Xu - Simon, Sibu - Kaufmann, Walter A. - De Rycke, Riet - Nodzynski, Tomasz - Zažímalová, Eva - Friml, Jiří
PY  - 2015
TI  - Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles
JF  - Journal of Experimental Botany
VL  - 66
IS  - 16
SP  - 5055-5065
EP  - 5055-5065
PB  - OXFORD UNIV PRESS
SN  - 00220957
KW  - Auxin
KW  - ABP1
KW  - Auxin binding
KW  - Site-directed mutagenesis
KW  - PIN proteins
UR  - http://jxb.oxfordjournals.org/content/66/16/5055.abstract
L2  - http://jxb.oxfordjournals.org/content/66/16/5055.abstract
N2  - The plant hormone auxin is a key regulator of plant growth and development. Auxin levels are sensed and interpreted by distinct receptor systems that activate a broad range of cellular responses. The Auxin-Binding Protein1 (ABP1) that has been identified based on its ability to bind auxin with high affinity is a prime candidate for the extracellular receptor responsible for mediating a range of auxin effects, in particular, the fast non-transcriptional ones. Contradictory genetic studies suggested prominent or no importance of ABP1 in many developmental processes. However, how crucial the role of auxin binding to ABP1 is for its functions has not been addressed. Here, we show that the auxinbinding pocket of ABP1 is essential for its gain-of-function cellular and developmental roles. In total, 16 different abp1 mutants were prepared that possessed substitutions in the metal core or in the hydrophobic amino acids of the auxin-binding pocket as well as neutral mutations. Their analysis revealed that an intact auxin-binding pocket is a prerequisite for ABP1 to activate downstream components of the ABP1 signalling pathway, such as Rho of Plants (ROPs) and to mediate the clathrin association with membranes for endocytosis regulation. In planta analyses demonstrated the importance of the auxin binding pocket for all known ABP1-mediated postembryonic developmental processes, including morphology of leaf epidermal cells, root growth and root meristem activity, and vascular tissue differentiation. Taken together, these findings suggest that auxin binding to ABP1 is central to its function, supporting the role of ABP1 as auxin receptor.
ER  -

GRONES, Peter; Xu CHEN; Sibu SIMON; Walter A. KAUFMANN; Riet DE RYCKE; Tomasz NODZYNSKI; Eva ZAŽÍMALOVÁ a Jiří FRIML. Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. \textit{Journal of Experimental Botany}. Oxford: OXFORD UNIV PRESS, 2015, roč.~66, č.~16, s.~5055-5065. ISSN~0022-0957. Dostupné z: https://dx.doi.org/10.1093/jxb/erv177.

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