Osmotic Stress Modulates the Balance between Exocytosis and
Clathrin-Mediated Endocytosis in Arabidopsis thaliana

J 2015

Osmotic Stress Modulates the Balance between Exocytosis and Clathrin-Mediated Endocytosis in Arabidopsis thaliana

ZWIEWKA, Marta; Tomasz NODZYNSKI; Stephanie ROBERT; Steffen VANNESTE; Jiří FRIML et. al.

Základní údaje

Originální název

Osmotic Stress Modulates the Balance between Exocytosis and Clathrin-Mediated Endocytosis in Arabidopsis thaliana

Autoři

ZWIEWKA, Marta (616 Polsko, domácí); Tomasz NODZYNSKI (616 Polsko, domácí); Stephanie ROBERT (56 Belgie); Steffen VANNESTE (56 Belgie) a Jiří FRIML (203 Česká republika, garant, domácí)

Vydání

Molecular Plant, Cambridge (USA), Cell Press, 2015, 1674-2052

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.142

Kód RIV

RIV/00216224:14740/15:00081166

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.molp.2015.03.007

UT WoS

000359136100006

EID Scopus

2-s2.0-84938751528

Klíčová slova anglicky

osmotic stress; protein trafficking; clathrin-mediated endocytosis; auxin

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 4. 2016 13:42, Mgr. Eva Špillingová

Anotace

V originále

The sessile life style of plants creates the need to deal with an often adverse environment, in which water availability can change on a daily basis, challenging the cellular physiology and integrity. Changes in osmotic conditions disrupt the equilibrium of the plasma membrane: hypoosmotic conditions increase and hyperosmotic environment decrease the cell volume. Here, we show that short-term extracellular osmotic treatments are closely followed by a shift in the balance between endocytosis and exocytosis in root meristem cells. Acute hyperosmotic treatments (ionic and nonionic) enhance clathrin-mediated endocytosis simultaneously attenuating exocytosis, whereas hypoosmotic treatments have the opposite effects. In addition to clathrin recruitment to the plasma membrane, components of early endocytic trafficking are essential during hyperosmotic stress responses. Consequently, growth of seedlings defective in elements of clathrin or early endocytic machinery is more sensitive to hyperosmotic treatments. We also found that the endocytotic response to a change of osmotic status in the environment is dominant over the presumably evolutionary more recent regulatory effect of plant hormones, such as auxin. These results imply that osmotic perturbation influences the balance between endocytosis and exocytosis acting through clathrin-mediated endocytosis. We propose that tension on the plasma membrane determines the addition or removal of membranes at the cell surface, thus preserving cell integrity.

Návaznosti

EE2.3.20.0043, projekt VaV

Název: Rozvoj lidských zdrojů pro výzkum, vývoj a inovace v oblasti genomiky a proteomiky rostlinných systémů

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GA13-40637S, projekt VaV

Název: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Grantová agentura ČR, Genetické studie k identifikaci molekulárních mechanizmu bunecné polarity a auxinového transportu v rostlinách

Citovat

ZWIEWKA, Marta; Tomasz NODZYNSKI; Stephanie ROBERT; Steffen VANNESTE a Jiří FRIML. Osmotic Stress Modulates the Balance between Exocytosis and Clathrin-Mediated Endocytosis in Arabidopsis thaliana. Molecular Plant. Cambridge (USA): Cell Press, 2015, roč. 8, č. 8, s. 1175-1187. ISSN 1674-2052. Dostupné z: https://dx.doi.org/10.1016/j.molp.2015.03.007.

@article{1315670,
   author = {Zwiewka, Marta and Nodzynski, Tomasz and Robert, Stephanie and Vanneste, Steffen and Friml, Jiří},
   article_location = {Cambridge (USA)},
   article_number = {8},
   doi = {http://dx.doi.org/10.1016/j.molp.2015.03.007},
   keywords = {osmotic stress; protein trafficking; clathrin-mediated endocytosis; auxin},
   language = {eng},
   issn = {1674-2052},
   journal = {Molecular Plant},
   title = {Osmotic Stress Modulates the Balance between Exocytosis and Clathrin-Mediated Endocytosis in Arabidopsis thaliana},
   url = {http://ac.els-cdn.com/S1674205215001756/1-s2.0-S1674205215001756-main.pdf?_tid=20ada804-7eed-11e5-9ff1-00000aacb35e&acdnat=1446199442_8b5124fda4cff4ecfa95b0073004473c},
   volume = {8},
   year = {2015}
}

TY  - JOUR
ID  - 1315670
AU  - Zwiewka, Marta - Nodzynski, Tomasz - Robert, Stephanie - Vanneste, Steffen - Friml, Jiří
PY  - 2015
TI  - Osmotic Stress Modulates the Balance between Exocytosis and Clathrin-Mediated Endocytosis in Arabidopsis thaliana
JF  - Molecular Plant
VL  - 8
IS  - 8
SP  - 1175-1187
EP  - 1175-1187
PB  - Cell Press
SN  - 16742052
KW  - osmotic stress
KW  - protein trafficking
KW  - clathrin-mediated endocytosis
KW  - auxin
UR  - http://ac.els-cdn.com/S1674205215001756/1-s2.0-S1674205215001756-main.pdf?_tid=20ada804-7eed-11e5-9ff1-00000aacb35e&acdnat=1446199442_8b5124fda4cff4ecfa95b0073004473c
L2  - http://ac.els-cdn.com/S1674205215001756/1-s2.0-S1674205215001756-main.pdf?_tid=20ada804-7eed-11e5-9ff1-00000aacb35e&acdnat=1446199442_8b5124fda4cff4ecfa95b0073004473c
N2  - The sessile life style of plants creates the need to deal with an often adverse environment, in which water availability can change on a daily basis, challenging the cellular physiology and integrity. Changes in osmotic conditions disrupt the equilibrium of the plasma membrane: hypoosmotic conditions increase and hyperosmotic environment decrease the cell volume. Here, we show that short-term extracellular osmotic treatments are closely followed by a shift in the balance between endocytosis and exocytosis in root meristem cells. Acute hyperosmotic treatments (ionic and nonionic) enhance clathrin-mediated endocytosis simultaneously attenuating exocytosis, whereas hypoosmotic treatments have the opposite effects. In addition to clathrin recruitment to the plasma membrane, components of early endocytic trafficking are essential during hyperosmotic stress responses. Consequently, growth of seedlings defective in elements of clathrin or early endocytic machinery is more sensitive to hyperosmotic treatments. We also found that the endocytotic response to a change of osmotic status in the environment is dominant over the presumably evolutionary more recent regulatory effect of plant hormones, such as auxin. These results imply that osmotic perturbation influences the balance between endocytosis and exocytosis acting through clathrin-mediated endocytosis. We propose that tension on the plasma membrane determines the addition or removal of membranes at the cell surface, thus preserving cell integrity.
ER  -

ZWIEWKA, Marta; Tomasz NODZYNSKI; Stephanie ROBERT; Steffen VANNESTE a Jiří FRIML. Osmotic Stress Modulates the Balance between Exocytosis and Clathrin-Mediated Endocytosis in Arabidopsis thaliana. \textit{Molecular Plant}. Cambridge (USA): Cell Press, 2015, roč.~8, č.~8, s.~1175-1187. ISSN~1674-2052. Dostupné z: https://dx.doi.org/10.1016/j.molp.2015.03.007.

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