Application of Molecular Diagnostics in Primary Detection of
ESBL Directly from Clinical Specimens

J 2015

Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens

SITTOVÁ, Martina; Magdaléna RÖDEROVÁ; Miloš DENDIS; Kristýna HRICOVÁ; Vendula PUDOVÁ et al.

Základní údaje

Originální název

Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens

Autoři

SITTOVÁ, Martina; Magdaléna RÖDEROVÁ; Miloš DENDIS; Kristýna HRICOVÁ; Vendula PUDOVÁ; Radek HORVÁTH; Filip RŮŽIČKA ; Šárka DOSOUDILOVÁ a Milan KOLÁŘ

Vydání

Microbial Drug Resistance, New Rochelle, Mary Ann Liebert, 2015, 1076-6294

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.529

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/15:00084517

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

SPECTRUM BETA-LACTAMASES; MULTIPLEX PCR; PSEUDOMONAS-AERUGINOSA; KLEBSIELLA-PNEUMONIAE; ESCHERICHIA-COLI; ENTEROBACTERIACEAE; RESISTANCE; GENES; INFECTIONS; SHV

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 11. 2015 16:21, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

The infections caused by extended-spectrum beta-lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients’ clinical material, was developed. This study focused on blaCTX-M and blaSHV determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using realtime PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the blaCTX-M. In two samples, the ESBL blaSHV-ESBL gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables detection of ESBL in 6 hours. The rapid method is necessary for early and adequate antimicrobial treatment.

Citovat

SITTOVÁ, Martina; Magdaléna RÖDEROVÁ; Miloš DENDIS; Kristýna HRICOVÁ; Vendula PUDOVÁ; Radek HORVÁTH; Filip RŮŽIČKA; Šárka DOSOUDILOVÁ a Milan KOLÁŘ. Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens. Microbial Drug Resistance. New Rochelle: Mary Ann Liebert, 2015, roč. 21, č. 3, s. 352-357. ISSN 1076-6294. Dostupné z: https://doi.org/10.1089/mdr.2014.0210.

@article{1315976,
   author = {Sittová, Martina and Röderová, Magdaléna and Dendis, Miloš and Hricová, Kristýna and Pudová, Vendula and Horváth, Radek and Růžička, Filip and Dosoudilová, Šárka and Kolář, Milan},
   article_location = {New Rochelle},
   article_number = {3},
   doi = {https://doi.org/10.1089/mdr.2014.0210},
   keywords = {SPECTRUM BETA-LACTAMASES; MULTIPLEX PCR; PSEUDOMONAS-AERUGINOSA; KLEBSIELLA-PNEUMONIAE; ESCHERICHIA-COLI; ENTEROBACTERIACEAE; RESISTANCE; GENES; INFECTIONS; SHV},
   language = {eng},
   issn = {1076-6294},
   journal = {Microbial Drug Resistance},
   title = {Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens},
   volume = {21},
   year = {2015}
}

TY  - JOUR
ID  - 1315976
AU  - Sittová, Martina - Röderová, Magdaléna - Dendis, Miloš - Hricová, Kristýna - Pudová, Vendula - Horváth, Radek - Růžička, Filip - Dosoudilová, Šárka - Kolář, Milan
PY  - 2015
TI  - Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens
JF  - Microbial Drug Resistance
VL  - 21
IS  - 3
SP  - 352-357
EP  - 352-357
PB  - Mary Ann Liebert
SN  - 10766294
KW  - SPECTRUM BETA-LACTAMASES
KW  - MULTIPLEX PCR
KW  - PSEUDOMONAS-AERUGINOSA
KW  - KLEBSIELLA-PNEUMONIAE
KW  - ESCHERICHIA-COLI
KW  - ENTEROBACTERIACEAE
KW  - RESISTANCE
KW  - GENES
KW  - INFECTIONS
KW  - SHV
N2  - The infections caused by extended-spectrum beta-lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients’ clinical material, was developed. This study focused on blaCTX-M and blaSHV determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using realtime PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the blaCTX-M. In two samples, the ESBL blaSHV-ESBL gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables detection of ESBL in 6 hours. The rapid method is necessary for early and adequate antimicrobial treatment.
ER  -

SITTOVÁ, Martina; Magdaléna RÖDEROVÁ; Miloš DENDIS; Kristýna HRICOVÁ; Vendula PUDOVÁ; Radek HORVÁTH; Filip RŮŽIČKA; Šárka DOSOUDILOVÁ a Milan KOLÁŘ. Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens. \textit{Microbial Drug Resistance}. New Rochelle: Mary Ann Liebert, 2015, roč.~21, č.~3, s.~352-357. ISSN~1076-6294. Dostupné z: https://doi.org/10.1089/mdr.2014.0210.

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