Application of Molecular Diagnostics in Primary Detection of
ESBL Directly from Clinical Specimens

J 2015

Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens

SITTOVÁ, Martina, Magdaléna RÖDEROVÁ, Miloš DENDIS, Kristýna HRICOVÁ, Vendula PUDOVÁ et. al.

Základní údaje

Originální název

Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens

Autoři

SITTOVÁ, Martina (203 Česká republika, domácí), Magdaléna RÖDEROVÁ (203 Česká republika), Miloš DENDIS (203 Česká republika), Kristýna HRICOVÁ (203 Česká republika), Vendula PUDOVÁ (203 Česká republika), Radek HORVÁTH (203 Česká republika), Filip RŮŽIČKA (203 Česká republika, garant, domácí), Šárka DOSOUDILOVÁ (203 Česká republika) a Milan KOLÁŘ (203 Česká republika)

Vydání

Microbial Drug Resistance, New Rochelle, Mary Ann Liebert, 2015, 1076-6294

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.529

Kód RIV

RIV/00216224:14110/15:00084517

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1089/mdr.2014.0210

UT WoS

000363875600015

Klíčová slova anglicky

SPECTRUM BETA-LACTAMASES; MULTIPLEX PCR; PSEUDOMONAS-AERUGINOSA; KLEBSIELLA-PNEUMONIAE; ESCHERICHIA-COLI; ENTEROBACTERIACEAE; RESISTANCE; GENES; INFECTIONS; SHV

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 11. 2015 16:21, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

The infections caused by extended-spectrum beta-lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients’ clinical material, was developed. This study focused on blaCTX-M and blaSHV determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using realtime PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the blaCTX-M. In two samples, the ESBL blaSHV-ESBL gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables detection of ESBL in 6 hours. The rapid method is necessary for early and adequate antimicrobial treatment.

Citovat

SITTOVÁ, Martina, Magdaléna RÖDEROVÁ, Miloš DENDIS, Kristýna HRICOVÁ, Vendula PUDOVÁ, Radek HORVÁTH, Filip RŮŽIČKA, Šárka DOSOUDILOVÁ a Milan KOLÁŘ. Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens. Microbial Drug Resistance. New Rochelle: Mary Ann Liebert, 2015, roč. 21, č. 3, s. 352-357. ISSN 1076-6294. Dostupné z: https://dx.doi.org/10.1089/mdr.2014.0210.

@article{1315976,
   author = {Sittová, Martina and Röderová, Magdaléna and Dendis, Miloš and Hricová, Kristýna and Pudová, Vendula and Horváth, Radek and Růžička, Filip and Dosoudilová, Šárka and Kolář, Milan},
   article_location = {New Rochelle},
   article_number = {3},
   doi = {http://dx.doi.org/10.1089/mdr.2014.0210},
   keywords = {SPECTRUM BETA-LACTAMASES; MULTIPLEX PCR; PSEUDOMONAS-AERUGINOSA; KLEBSIELLA-PNEUMONIAE; ESCHERICHIA-COLI; ENTEROBACTERIACEAE; RESISTANCE; GENES; INFECTIONS; SHV},
   language = {eng},
   issn = {1076-6294},
   journal = {Microbial Drug Resistance},
   title = {Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens},
   volume = {21},
   year = {2015}
}

TY  - JOUR
ID  - 1315976
AU  - Sittová, Martina - Röderová, Magdaléna - Dendis, Miloš - Hricová, Kristýna - Pudová, Vendula - Horváth, Radek - Růžička, Filip - Dosoudilová, Šárka - Kolář, Milan
PY  - 2015
TI  - Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens
JF  - Microbial Drug Resistance
VL  - 21
IS  - 3
SP  - 352-357
EP  - 352-357
PB  - Mary Ann Liebert
SN  - 10766294
KW  - SPECTRUM BETA-LACTAMASES
KW  - MULTIPLEX PCR
KW  - PSEUDOMONAS-AERUGINOSA
KW  - KLEBSIELLA-PNEUMONIAE
KW  - ESCHERICHIA-COLI
KW  - ENTEROBACTERIACEAE
KW  - RESISTANCE
KW  - GENES
KW  - INFECTIONS
KW  - SHV
N2  - The infections caused by extended-spectrum beta-lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients’ clinical material, was developed. This study focused on blaCTX-M and blaSHV determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using realtime PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the blaCTX-M. In two samples, the ESBL blaSHV-ESBL gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables detection of ESBL in 6 hours. The rapid method is necessary for early and adequate antimicrobial treatment.
ER  -

SITTOVÁ, Martina, Magdaléna RÖDEROVÁ, Miloš DENDIS, Kristýna HRICOVÁ, Vendula PUDOVÁ, Radek HORVÁTH, Filip RŮŽIČKA, Šárka DOSOUDILOVÁ a Milan KOLÁŘ. Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens. \textit{Microbial Drug Resistance}. New Rochelle: Mary Ann Liebert, 2015, roč.~21, č.~3, s.~352-357. ISSN~1076-6294. Dostupné z: https://dx.doi.org/10.1089/mdr.2014.0210.

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