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@article{1315976,
author = {Sittová, Martina and Röderová, Magdaléna and Dendis, Miloš and Hricová, Kristýna and Pudová, Vendula and Horváth, Radek and Růžička, Filip and Dosoudilová, Šárka and Kolář, Milan},
article_location = {New Rochelle},
article_number = {3},
doi = {http://dx.doi.org/10.1089/mdr.2014.0210},
keywords = {SPECTRUM BETA-LACTAMASES; MULTIPLEX PCR; PSEUDOMONAS-AERUGINOSA; KLEBSIELLA-PNEUMONIAE; ESCHERICHIA-COLI; ENTEROBACTERIACEAE; RESISTANCE; GENES; INFECTIONS; SHV},
language = {eng},
issn = {1076-6294},
journal = {Microbial Drug Resistance},
title = {Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens},
volume = {21},
year = {2015}
}
TY - JOUR
ID - 1315976
AU - Sittová, Martina - Röderová, Magdaléna - Dendis, Miloš - Hricová, Kristýna - Pudová, Vendula - Horváth, Radek - Růžička, Filip - Dosoudilová, Šárka - Kolář, Milan
PY - 2015
TI - Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens
JF - Microbial Drug Resistance
VL - 21
IS - 3
SP - 352-357
EP - 352-357
PB - Mary Ann Liebert
SN - 10766294
KW - SPECTRUM BETA-LACTAMASES
KW - MULTIPLEX PCR
KW - PSEUDOMONAS-AERUGINOSA
KW - KLEBSIELLA-PNEUMONIAE
KW - ESCHERICHIA-COLI
KW - ENTEROBACTERIACEAE
KW - RESISTANCE
KW - GENES
KW - INFECTIONS
KW - SHV
N2 - The infections caused by extended-spectrum beta-lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients’ clinical material, was developed. This study focused on blaCTX-M and blaSHV determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using realtime PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the blaCTX-M. In two samples, the ESBL blaSHV-ESBL gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables detection of ESBL in 6 hours. The rapid method is necessary for early and adequate antimicrobial treatment.
ER -
SITTOVÁ, Martina, Magdaléna RÖDEROVÁ, Miloš DENDIS, Kristýna HRICOVÁ, Vendula PUDOVÁ, Radek HORVÁTH, Filip RŮŽIČKA, Šárka DOSOUDILOVÁ a Milan KOLÁŘ. Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens. \textit{Microbial Drug Resistance}. New Rochelle: Mary Ann Liebert, roč.~21, č.~3, s.~352-357. ISSN~1076-6294. doi:10.1089/mdr.2014.0210. 2015.
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