LUKAS, J, P MAZNA, T VALENTA, L DOUBRAVSKA, Vendula POSPÍCHALOVÁ, M VOJTECHOVA, Bohumil FAFÍLEK, R IVANEK, J PLACHY, J NOVAK a V KORINEK. Dazap2 modulates transcription driven by the Wnt effector TCF-4. Nucleic Acids Research. Oxford, UK: Oxford Press, 2009, roč. 37, č. 9, s. 3007-3020. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkp179. |
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@article{1316072, author = {Lukas, J and Mazna, P and Valenta, T and Doubravska, L and Pospíchalová, Vendula and Vojtechova, M and Fafílek, Bohumil and Ivanek, R and Plachy, J and Novak, J and Korinek, V}, article_location = {Oxford, UK}, article_number = {9}, doi = {http://dx.doi.org/10.1093/nar/gkp179}, language = {eng}, issn = {0305-1048}, journal = {Nucleic Acids Research}, title = {Dazap2 modulates transcription driven by the Wnt effector TCF-4}, volume = {37}, year = {2009} }
TY - JOUR ID - 1316072 AU - Lukas, J - Mazna, P - Valenta, T - Doubravska, L - Pospíchalová, Vendula - Vojtechova, M - Fafílek, Bohumil - Ivanek, R - Plachy, J - Novak, J - Korinek, V PY - 2009 TI - Dazap2 modulates transcription driven by the Wnt effector TCF-4 JF - Nucleic Acids Research VL - 37 IS - 9 SP - 3007-3020 EP - 3007-3020 PB - Oxford Press SN - 03051048 N2 - A major outcome of the canonical Wnt/-catenin-signalling pathway is the transcriptional activation of a specific set of target genes. A typical feature of the transcriptional response induced by Wnt signalling is the involvement of Tcf/Lef factors that function in the nucleus as the principal mediators of signalling. Vertebrate Tcf/Lef proteins perform two well-characterized functions: in association with -catenin they activate gene expression, and in the absence of Wnt ligands they bind TLE/Groucho proteins to act as transcriptional repressors. Although the general characteristics of Tcf/Lef factors are well understood, the mechanisms that control their specific roles in various cellular backgrounds are much less defined. In this report we reveal that the evolutionary conserved Dazap2 protein functions as a TCF-4 interacting partner. We demonstrate that a short region proximal to the TCF-4 HMG box mediates the interaction and that all Tcf/Lef family members associate with Dazap2. Interestingly, knockdown of Dazap2 not only reduced the activity of Wnt signalling as measured by Tcf/-catenin reporters but additionally altered the expression of Wnt-signalling target genes. Finally, chromatin immunoprecipitation studies indicate that Dazap2 modulates the affinity of TCF-4 for its DNA-recognition motif. ER -
LUKAS, J, P MAZNA, T VALENTA, L DOUBRAVSKA, Vendula POSPÍCHALOVÁ, M VOJTECHOVA, Bohumil FAFÍLEK, R IVANEK, J PLACHY, J NOVAK a V KORINEK. Dazap2 modulates transcription driven by the Wnt effector TCF-4. \textit{Nucleic Acids Research}. Oxford, UK: Oxford Press, 2009, roč.~37, č.~9, s.~3007-3020. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkp179.
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