Dazap2 modulates transcription driven by the Wnt effector TCF-4

J 2009

Dazap2 modulates transcription driven by the Wnt effector TCF-4

LUKAS, J; P MAZNA; T VALENTA; L DOUBRAVSKA; Vendula POSPÍCHALOVÁ et al.

Základní údaje

Originální název

Dazap2 modulates transcription driven by the Wnt effector TCF-4

Autoři

LUKAS, J; P MAZNA; T VALENTA; L DOUBRAVSKA; Vendula POSPÍCHALOVÁ; M VOJTECHOVA; Bohumil FAFÍLEK; R IVANEK; J PLACHY; J NOVAK a V KORINEK

Vydání

Nucleic Acids Research, Oxford, UK, Oxford Press, 2009, 0305-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 7.479

Označené pro přenos do RIV

DOI

https://doi.org/10.1093/nar/gkp179

UT WoS

000266354600021

Změněno: 3. 11. 2015 22:54, RNDr. Vendula Hlaváčková Pospíchalová, Ph.D.

Anotace

V originále

A major outcome of the canonical Wnt/-catenin-signalling pathway is the transcriptional activation of a specific set of target genes. A typical feature of the transcriptional response induced by Wnt signalling is the involvement of Tcf/Lef factors that function in the nucleus as the principal mediators of signalling. Vertebrate Tcf/Lef proteins perform two well-characterized functions: in association with -catenin they activate gene expression, and in the absence of Wnt ligands they bind TLE/Groucho proteins to act as transcriptional repressors. Although the general characteristics of Tcf/Lef factors are well understood, the mechanisms that control their specific roles in various cellular backgrounds are much less defined. In this report we reveal that the evolutionary conserved Dazap2 protein functions as a TCF-4 interacting partner. We demonstrate that a short region proximal to the TCF-4 HMG box mediates the interaction and that all Tcf/Lef family members associate with Dazap2. Interestingly, knockdown of Dazap2 not only reduced the activity of Wnt signalling as measured by Tcf/-catenin reporters but additionally altered the expression of Wnt-signalling target genes. Finally, chromatin immunoprecipitation studies indicate that Dazap2 modulates the affinity of TCF-4 for its DNA-recognition motif.

Citovat

LUKAS, J; P MAZNA; T VALENTA; L DOUBRAVSKA; Vendula POSPÍCHALOVÁ; M VOJTECHOVA; Bohumil FAFÍLEK; R IVANEK; J PLACHY; J NOVAK a V KORINEK. Dazap2 modulates transcription driven by the Wnt effector TCF-4. Nucleic Acids Research. Oxford, UK: Oxford Press, 2009, roč. 37, č. 9, s. 3007-3020. ISSN 0305-1048. Dostupné z: https://doi.org/10.1093/nar/gkp179.

@article{1316072,
   author = {Lukas, J and Mazna, P and Valenta, T and Doubravska, L and Pospíchalová, Vendula and Vojtechova, M and Fafílek, Bohumil and Ivanek, R and Plachy, J and Novak, J and Korinek, V},
   article_location = {Oxford, UK},
   article_number = {9},
   doi = {https://doi.org/10.1093/nar/gkp179},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic Acids Research},
   title = {Dazap2 modulates transcription driven by the Wnt effector TCF-4},
   volume = {37},
   year = {2009}
}

TY  - JOUR
ID  - 1316072
AU  - Lukas, J - Mazna, P - Valenta, T - Doubravska, L - Pospíchalová, Vendula - Vojtechova, M - Fafílek, Bohumil - Ivanek, R - Plachy, J - Novak, J - Korinek, V
PY  - 2009
TI  - Dazap2 modulates transcription driven by the Wnt effector TCF-4
JF  - Nucleic Acids Research
VL  - 37
IS  - 9
SP  - 3007-3020
EP  - 3007-3020
PB  - Oxford Press
SN  - 03051048
N2  - A major outcome of the canonical Wnt/-catenin-signalling pathway is the transcriptional activation of a specific set of target genes. A typical feature of the transcriptional response induced by Wnt signalling is the involvement of Tcf/Lef factors that function in the nucleus as the principal mediators of signalling. Vertebrate Tcf/Lef proteins perform two well-characterized functions: in association with -catenin they activate gene expression, and in the absence of Wnt ligands they bind TLE/Groucho proteins to act as transcriptional repressors. Although the general characteristics of Tcf/Lef factors are well understood, the mechanisms that control their specific roles in various cellular backgrounds are much less defined. In this report we reveal that the evolutionary conserved Dazap2 protein functions as a TCF-4 interacting partner. We demonstrate that a short region proximal to the TCF-4 HMG box mediates the interaction and that all Tcf/Lef family members associate with Dazap2. Interestingly, knockdown of Dazap2 not only reduced the activity of Wnt signalling as measured by Tcf/-catenin reporters but additionally altered the expression of Wnt-signalling target genes. Finally, chromatin immunoprecipitation studies indicate that Dazap2 modulates the affinity of TCF-4 for its DNA-recognition motif.
ER  -

LUKAS, J; P MAZNA; T VALENTA; L DOUBRAVSKA; Vendula POSPÍCHALOVÁ; M VOJTECHOVA; Bohumil FAFÍLEK; R IVANEK; J PLACHY; J NOVAK a V KORINEK. Dazap2 modulates transcription driven by the Wnt effector TCF-4. \textit{Nucleic Acids Research}. Oxford, UK: Oxford Press, 2009, roč.~37, č.~9, s.~3007-3020. ISSN~0305-1048. Dostupné z: https://doi.org/10.1093/nar/gkp179.

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