Další formáty:
BibTeX
LaTeX
RIS
@article{1316836,
author = {Dadáková, Kateřina and Grochalová, Martina and Kurková, Barbora and Tlolková, Ivana and Kašparovský, Tomáš and Zdráhal, Zbyněk and Lochman, Jan},
article_location = {Amsterdam},
article_number = {April},
doi = {http://dx.doi.org/10.1016/j.jprot.2015.02.001},
keywords = {Vitis vinifera; Botrytis cinerea; Defence; Cytoplasmic proteome; Vacuolar proteome; Transcript analysis},
language = {eng},
issn = {1874-3919},
journal = {Journal of Proteomics},
title = {Proteome and transcript analysis of Vitis vinifera cell cultures subjected to Botrytis cinerea infection},
volume = {119},
year = {2015}
}
TY - JOUR
ID - 1316836
AU - Dadáková, Kateřina - Grochalová, Martina - Kurková, Barbora - Tlolková, Ivana - Kašparovský, Tomáš - Zdráhal, Zbyněk - Lochman, Jan
PY - 2015
TI - Proteome and transcript analysis of Vitis vinifera cell cultures subjected to Botrytis cinerea infection
JF - Journal of Proteomics
VL - 119
IS - April
SP - 143-153
EP - 143-153
PB - Elsevier Science
SN - 18743919
KW - Vitis vinifera
KW - Botrytis cinerea
KW - Defence
KW - Cytoplasmic proteome
KW - Vacuolar proteome
KW - Transcript analysis
N2 - Gray mold caused by Botrytis cinerea is one of the most important diseases of grapevine resulting in significant reductions in yield and fruit quality. In order to examine the molecular mechanisms that characterize the interaction between B. cinerea and the host plant, the grapevine cytoplasmic proteome was analyzed by two-dimensional polyacrylamide gel electrophoresis. The interaction between Vitis vinifera cv. Gamay cells and B. cinerea was characterized by the increase in spot abundance of 30 proteins, of which 21 were successfully identified. The majority of these proteins were related to defence and stress responses and to cell wall modifications. Some of the modulated proteins have been previously found to be affected by other pathogens when they infect V. vinifera but interestingly, the proteins related to cell wall modification that were influenced by B. cinerea have not been shown to be modulated by any other pathogen studied to date. Transcript analysis using the quantitative real time polymerase chain reaction additionally revealed the up-regulation of several acidic, probably extracellular, chitinases. The results indicate that cell wall strengthening, accumulation of PR proteins and excretion of lytic enzymes are likely to be important mechanisms in the defence of grapevine against B. cinerea. Biological significance Although gray mold caused by Botrytis cinerea is one of the most important diseases of grapevine, little information is available about proteomic changes in this pathosystem. These results suggest that cell wall strengthening, accumulation of PR proteins and excretion of lytic enzymes are important molecular mechanisms in the defence of grapevine against B. cinerea. Surprisingly, the proteins related to cell wall modification that were modulated by B. cinerea have not been shown to be affected by any other pathogen studied to date. (C) 2015 Elsevier B.V. All rights reserved.
ER -
DADÁKOVÁ, Kateřina, Martina GROCHALOVÁ, Barbora KURKOVÁ, Ivana TLOLKOVÁ, Tomáš KAŠPAROVSKÝ, Zbyněk ZDRÁHAL a Jan LOCHMAN. Proteome and transcript analysis of Vitis vinifera cell cultures subjected to Botrytis cinerea infection. Online. \textit{Journal of Proteomics}. Amsterdam: Elsevier Science, 2015, roč.~119, April, s.~143-153. ISSN~1874-3919. Dostupné z: https://dx.doi.org/10.1016/j.jprot.2015.02.001. [citováno 2024-04-23]
|
