Další formáty:
BibTeX
LaTeX
RIS
@article{1316972,
author = {Zemová, Radka and Zwiewka, Marta and Bielach, Agnieszka and Robert Boisivon, Hélene and Friml, Jiří},
article_location = {New York},
article_number = {2},
doi = {http://dx.doi.org/10.1007/s00344-015-9553-2},
keywords = {Root development Polar auxin transport Plasma membrane AUX1 PIN2 Vacuolar degradation},
language = {eng},
issn = {0721-7595},
journal = {Journal of plant growth regulation},
title = {A Forward Genetic Screen for New Regulators of Auxin-mediated Degradation of Auxin Transport Proteins in Arabidopsis thaliana},
url = {http://link.springer.com/article/10.1007/s00344-015-9553-2},
volume = {35},
year = {2016}
}
TY - JOUR
ID - 1316972
AU - Zemová, Radka - Zwiewka, Marta - Bielach, Agnieszka - Robert Boisivon, Hélene - Friml, Jiří
PY - 2016
TI - A Forward Genetic Screen for New Regulators of Auxin-mediated Degradation of Auxin Transport Proteins in Arabidopsis thaliana
JF - Journal of plant growth regulation
VL - 35
IS - 2
SP - 465-476
EP - 465-476
PB - Springer
SN - 07217595
KW - Root development Polar auxin transport Plasma membrane AUX1 PIN2 Vacuolar degradation
UR - http://link.springer.com/article/10.1007/s00344-015-9553-2
L2 - http://link.springer.com/article/10.1007/s00344-015-9553-2
N2 - The plant hormone auxin (indole-3-acetic acid) is a major regulator of plant growth and development including embryo and root patterning, lateral organ formation and growth responses to environmental stimuli. Auxin is directionally transported from cell to cell by the action of specific auxin influx [AUXIN-RESISTANT1 (AUX1)] and efflux [PIN-FORMED (PIN)] transport regulators, whose polar, subcellular localizations are aligned with the direction of the auxin flow. Auxin itself regulates its own transport by modulation of the expression and subcellular localization of the auxin transporters. Increased auxin levels promote the transcription of PIN2 and AUX1 genes as well as stabilize PIN proteins at the plasma membrane, whereas prolonged auxin exposure increases the turnover of PIN proteins and their degradation in the vacuole. In this study, we applied a forward genetic approach, to identify molecular components playing a role in the auxin-mediated degradation. We generated EMS-mutagenized Arabidopsis PIN2::PIN2:GFP, AUX1::AUX1:YFP eir1 aux1 populations and designed a screen for mutants with persistently strong fluorescent signals of the tagged PIN2 and AUX1 after prolonged treatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D). This approach yielded novel auxin degradation mutants defective in trafficking and degradation of PIN2 and AUX1 proteins and established a role for auxin-mediated degradation in plant development.
ER -
ZEMOVÁ, Radka, Marta ZWIEWKA, Agnieszka BIELACH, Hélene ROBERT BOISIVON a Jiří FRIML. A Forward Genetic Screen for New Regulators of Auxin-mediated Degradation of Auxin Transport Proteins in Arabidopsis thaliana. \textit{Journal of plant growth regulation}. New York: Springer, 2016, roč.~35, č.~2, s.~465-476. ISSN~0721-7595. Dostupné z: https://dx.doi.org/10.1007/s00344-015-9553-2.
|
